2. Apoptosis
  3. Apoptosis Caspase
  4. Anti-Human/Mouse CD95 Antibody (HFE7A)

Anti-Human/Mouse CD95 Antibody (HFE7A) 

Cat. No.: HY-P992072
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Anti-Human/Mouse CD95 Antibody (HFE7A) is an antibody targeting human/mouse Fas (CD95), with a Kd of 1.6 nM in mice. Anti-Human/Mouse CD95 Antibody (HFE7A) modulates the Fas-mediated apoptotic signaling pathway without blocking the binding of Jo2 to Fas. Anti-Human/Mouse CD95 Antibody (HFE7A) inhibits Jo2-induced caspase activation, mitochondrial depolarization, hepatocyte death and apoptosis. Anti-Human/Mouse CD95 Antibody (HFE7A) protects BALB/c mice against Jo2-induced acute liver injury and reduces Jo2-associated elevation of serum transaminase levels. Anti-Human/Mouse CD95 Antibody (HFE7A) can be used in studies related to liver injury. For isotype control, refer to Mouse IgG1 kappa, Isotype Control (HY-P99977).

For research use only. We do not sell to patients.

Anti-Human/Mouse CD95 Antibody (HFE7A)

Anti-Human/Mouse CD95 Antibody (HFE7A) Chemical Structure

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Anti-Human/Mouse CD95 Antibody (HFE7A) Related Antibodies

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Description

Anti-Human/Mouse CD95 Antibody (HFE7A) is an antibody targeting human/mouse Fas (CD95), with a Kd of 1.6 nM in mice. Anti-Human/Mouse CD95 Antibody (HFE7A) modulates the Fas-mediated apoptotic signaling pathway without blocking the binding of Jo2 to Fas. Anti-Human/Mouse CD95 Antibody (HFE7A) inhibits Jo2-induced caspase activation, mitochondrial depolarization, hepatocyte death and apoptosis. Anti-Human/Mouse CD95 Antibody (HFE7A) protects BALB/c mice against Jo2-induced acute liver injury and reduces Jo2-associated elevation of serum transaminase levels. Anti-Human/Mouse CD95 Antibody (HFE7A) can be used in studies related to liver injury. For isotype control, refer to Mouse IgG1 kappa, Isotype Control (HY-P99977)[1].

Isotype

Mouse IgG1 kappa
Recommend Isotype Controls
Species Reactivity

Human/Mouse
In Vitro

Anti-Human/Mouse CD95 Antibody (HFE7A) (0.05-5 μg/mL; 1.5-6 h) inhibits Jo2-induced death of primary BALB/c mouse hepatocytes in vitro in a dose-dependent manner, as well as the activation of caspase-3, -8 and -9[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Human/Mouse CD95 Antibody (HFE7A) Related Antibodies

Cell Viability Assay[1]

Cell Line: Jo2 treated hepatocytes
Concentration: 0.05, 0.5, 5 μg/mL
Incubation Time: 1.5, 3, 6 h
Result: Inhibited cell death in a dose-dependent manner.
In Vivo

Anti-Human/Mouse CD95 Antibody (HFE7A) (16.5-200 μg; i.v.; single dose) completely rescues BALB/c mice from Jo2-induced lethal acute fulminant hepatitis and inhibits Jo2-induced hepatocyte apoptosis via suppression of caspase activation and mitochondrial depolarization[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c (female)[1]
Dosage: 67 μg (survival; AST/ALT inhibition); 16.5 μg (caspase suppression); 200 μg (mitochondrial depolarization reduction)
Administration: i.v.; single dose
Result: Achieved 100% survival (3/3 mice) at 4 and 24 hours post-Jo2 injection.
Inhibited Jo2-induced increases in serum AST and ALT levels.
Almost completely suppressed Jo2-induced activation of caspases-3, -8, and -9 in hepatocytes to basal levels.
Reduced the percentage of hepatocytes with mitochondrial depolarization when administered simultaneously with Jo2 at 200 μg.
Gene ID

355  [NCBI]

Accession

P25445
Target

Fas/CD95
Application

ELISA, FACS, Functional assay, Research in vivo
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
SMILES

[Anti-Human/Mouse CD95 Antibody (HFE7A)]
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Anti-Human/Mouse CD95 Antibody (HFE7A) Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Human/Mouse CD95 Antibody (HFE7A)ApoptosisCaspaseacute hepatic injurycaspase activationBALB/c micemitochondrial depolarizationapoptosisFas (CD95)Fas-mediated apoptotic signalingmouse thymocyteshepatocyte cell deathhuman rheumatoid arthritis synovial cellsInhibitorinhibitorinhibit

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