Anti-Human/Mouse CD95 Antibody (HFE7A)
Anti-Human/Mouse CD95 Antibody (HFE7A) is an antibody targeting human/mouse Fas (CD95), with a Kd of 1.6 nM in mice. Anti-Human/Mouse CD95 Antibody (HFE7A) modulates the Fas-mediated apoptotic signaling pathway without blocking the binding of Jo2 to Fas. Anti-Human/Mouse CD95 Antibody (HFE7A) inhibits Jo2-induced caspase activation, mitochondrial depolarization, hepatocyte death and apoptosis. Anti-Human/Mouse CD95 Antibody (HFE7A) protects BALB/c mice against Jo2-induced acute liver injury and reduces Jo2-associated elevation of serum transaminase levels. Anti-Human/Mouse CD95 Antibody (HFE7A) can be used in studies related to liver injury. For isotype control, refer to Mouse IgG1 kappa, Isotype Control (HY-P99977).
For research use only. We do not sell to patients.
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Mouse IgG1 kappa
Human/Mouse
Anti-Human/Mouse CD95 Antibody (HFE7A) (0.05-5 μg/mL; 1.5-6 h) inhibits Jo2-induced death of primary BALB/c mouse hepatocytes in vitro in a dose-dependent manner, as well as the activation of caspase-3, -8 and -9[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Jo2 treated hepatocytes
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Concentration:0.05, 0.5, 5 μg/mL
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Incubation Time:1.5, 3, 6 h
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Result:Inhibited cell death in a dose-dependent manner.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c (female)[1]
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Dosage:67 μg (survival; AST/ALT inhibition); 16.5 μg (caspase suppression); 200 μg (mitochondrial depolarization reduction)
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Administration:i.v.; single dose
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Result:Achieved 100% survival (3/3 mice) at 4 and 24 hours post-Jo2 injection.
Inhibited Jo2-induced increases in serum AST and ALT levels.
Almost completely suppressed Jo2-induced activation of caspases-3, -8, and -9 in hepatocytes to basal levels.
Reduced the percentage of hepatocytes with mitochondrial depolarization when administered simultaneously with Jo2 at 200 μg.
P25445
Fas/CD95
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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Product Image
ELISA, FACS, Functional assay, Research in vivo
Chemical Information
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SMILES
[Anti-Human/Mouse CD95 Antibody (HFE7A)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Human/Mouse CD95 Antibody (HFE7A) Related Classifications
HY-P992072 Related Classifications
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