Anti-Mouse/Rat/Human CCL2/MCP-1 Antibody (2H5)
Based on 1 publication(s) in Google Scholar
Anti-Mouse/Rat/Human CCL2/MCP-1 Antibody (2H5) is an anti-mouse/rat/human CCL2/MCP-1 IgG monoclonal antibody. Anti-Mouse/Rat/Human CCL2/MCP-1 Antibody (2H5) can reverse the immunosuppressive microenvironment by blocking the CCL2 signaling pathway. Anti-Mouse/Rat/Human CCL2/MCP-1 Antibody (2H5) can reduce the secretion of IFN-γ and the infiltration of macrophages. CCL2/MCP-1 Antibody (2H5) can reduce the amount of HIV virus by increasing the proportion of T cells. Anti-Mouse/Rat/Human CCL2/MCP-1 Antibody (2H5) can be used for researches on inflammation conditions, virus infection and cancer such as inflammatory bowel disease (IBD), hepatocellular carcinoma (HCC) and HIV.
For research use only. We do not sell to patients.
- Purity : 99.5%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Anti-Mouse/Rat/Human CCL2/MCP-1 Antibody (2H5)
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Biological Activity
Description
Isotype
Armenian hamster IgG
Recommend Isotype Controls
Species Reactivity
Human/Mouse/Rat
IC50 & Target
CCL2/MCP-1
In Vitro
Anti-Mouse/Rat/Human CCL2/MCP-1 Antibody (2H5) (5 μg/mL, 16 h) significantly reduces the migration of THP-1 cells to infected enteroid-derived monolayers (EDMs)[1].
Anti-Mouse/Rat/Human CCL2/MCP-1 Antibody (2H5) (10 μg/mL, 24 h) significantly reduces the enhancing effect of mesenchymal stromal/stem cells (MSCs) on natural killer (NK) cell IFN-γ secretion[2].
Anti-Mouse/Rat/Human CCL2/MCP-1 Antibody (2H5) can inhibit the recruitment of CD8+ T cells by LILRB4 knockdown in BV2 cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:THP-1 cells
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Concentration:5 μg/mL
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Incubation Time:16 h
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Result:Significantly inhibited the migration of THP-1 cells.
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Cell Line:NK cells, co-culture with MSCs
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Concentration:10 μg/mL
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Incubation Time:24 h
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Result:Significantly reduced the level of IFN-γ.
In Vivo
Anti-Mouse/Rat/Human CCL2/MCP-1 Antibody (2H5) (100-200 μg, i.p., once every 48 h, for 12 days) significantly reduces the amount of HIV virus in TKO-BLT mice infected with HIV virus[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:DEN (20 mg/kg) and CCl4 (2 μL/g) induced C57BL/6J mice[4]
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Dosage:8 mg/kg, combined with BLZ945 (200 mg/kg)
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Administration:Intraperitoneal injection (i.p.), 4 times weekly from week 18 to week 24
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Result:Significantly reduced the number of tumors.
Reduced M2 macrophage infiltration (CD68+ and CD206+).
Had no significant change in weight.
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Animal Model:5×104 tissue culture infectious units (TCIU) HIV virus injected TKO-BLT mice[5]
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Dosage:100 μg for first-dose, 200 μg for subsequent doses
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Administration:Intraperitoneal injection (i.p.), once every 48 h for 12 days
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Result:Caused only 14 % of mice to detect HIV DNA, compared to 41 % in the control group.
Did not cause virus rebound but 5 mice showed virus rebound in the control group.
Significantly increased the proportion of CCR2/5+ CD4 T cells.
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Product Image
Application
in vivo CCL2 neutralization; Immunohistochemistry (frozen)
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse/Rat/Human CCL2/MCP-1 Antibody (2H5)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (1)
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Journal Impact Factor
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Most Recent
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Proc Natl Acad Sci U S A
Unraveling the neuroimmune mechanisms in cancer-induced bone pain: New horizons for therapeutic intervention of the two-phase paradigm. [Abstract]2025 Aug 26;122(34):e2503779122. PMID: 40828016
Purity & Documentation
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Data Sheet (267 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Sayed IM, et al. Host engulfment pathway controls inflammation in inflammatory bowel disease. FEBS J. 2020 Sep;287(18):3967-3988. [Content Brief]
[2]. Cui R, et al. Human mesenchymal stromal/stem cells acquire immunostimulatory capacity upon cross-talk with natural killer cells and might improve the NK cell function of immunocompromised patients. Stem Cell Res Ther. 2016 Jul 7;7(1):88. [Content Brief]
[3]. Ma Y, et al. Microglia LILRB4 upregulation reduces brain damage after acute ischemic stroke by limiting CD8+ T cell recruitment. J Neuroinflammation. 2024 Aug 31;21(1):214. [Content Brief]
[4]. Du Y, et al. ASH1L in Hepatoma Cells and Hepatic Stellate Cells Promotes Fibrosis-Associated Hepatocellular Carcinoma by Modulating Tumor-Associated Macrophages. Adv Sci (Weinh). 2024 Dec;11(45):e2404756. [Content Brief]
[5]. Packard TA, et al. CCL2: a Chemokine Potentially Promoting Early Seeding of the Latent HIV Reservoir. mBio. 2022 Oct 26;13(5):e0189122. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse/Rat/Human CCL2/MCP-1 Antibody (2H5) Related Classifications
HY-P990297 Related Classifications
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