2. PI3K/Akt/mTOR Stem Cell/Wnt Protein Tyrosine Kinase/RTK
  3. GSK-3 DYRK
  4. ARN25068

ARN25068 is a sub-micromolar inhibitor of the three protein kinases, GSK-3β, FYN and DYRK1A to tackle tau hyperphosphorylation.

For research use only. We do not sell to patients.

ARN25068 Chemical Structure

ARN25068 Chemical Structure

CAS No. : 2649882-80-2

Size Price Stock Quantity
5 mg USD 240 In-stock
10 mg USD 390 In-stock
25 mg USD 780 In-stock
50 mg USD 1250 In-stock
100 mg USD 2000 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

ARN25068 Related Antibodies

Top Publications Citing Use of Products

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GSK-3 GSK-3α GSK-3β

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DYRK1 DYRK2 DYRK4

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

ARN25068 is a sub-micromolar inhibitor of the three protein kinases, GSK-3β, FYN and DYRK1A to tackle tau hyperphosphorylation[1].

In Vitro

ARN25068 reduces the extent of tau phosphorylation in Tau0N4R-TM-tGFP U2OS cell line. ARN25068 exhibits IC50 values of 4.17 and 2.17 μM for GSK-3β and FYN in enzymatic radiometric assay, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ARN25068 Related Antibodies
Molecular Weight

362.45

Formula

C19H18N6S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C1(CNC2=NC(NC3=CC(C4CC4)=NN3)=C5SC=CC5=N2)=CC=CC=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 200 mg/mL (551.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7590 mL 13.7950 mL 27.5900 mL
5 mM 0.5518 mL 2.7590 mL 5.5180 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 5 mg/mL (13.80 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: 5 mg/mL (13.80 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7590 mL 13.7950 mL 27.5900 mL 68.9750 mL
5 mM 0.5518 mL 2.7590 mL 5.5180 mL 13.7950 mL
10 mM 0.2759 mL 1.3795 mL 2.7590 mL 6.8975 mL
15 mM 0.1839 mL 0.9197 mL 1.8393 mL 4.5983 mL
20 mM 0.1380 mL 0.6898 mL 1.3795 mL 3.4488 mL
25 mM 0.1104 mL 0.5518 mL 1.1036 mL 2.7590 mL
30 mM 0.0920 mL 0.4598 mL 0.9197 mL 2.2992 mL
40 mM 0.0690 mL 0.3449 mL 0.6898 mL 1.7244 mL
50 mM 0.0552 mL 0.2759 mL 0.5518 mL 1.3795 mL
60 mM 0.0460 mL 0.2299 mL 0.4598 mL 1.1496 mL
80 mM 0.0345 mL 0.1724 mL 0.3449 mL 0.8622 mL
100 mM 0.0276 mL 0.1380 mL 0.2759 mL 0.6898 mL
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ARN25068 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ARN250682649882-80-2ARN 25068ARN-25068GSK-3DYRKGlycogen synthase kinase-3Glycogen synthase kinase 3Dual specificity tyrosine phosphorylation regulated kinaseDual specificity tyrosine regulated kinaseGSK-3βFYNDYRK1Atau hyperphosphorylationInhibitorinhibitorinhibit

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