Art. -Nr.: HY-W001160

5-Hydroxyindole

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5-Hydroxyindole is an orally active hydroxylated indole and tryptophan metabolite. 5-Hydroxyindole activates α7 nicotinic acetylcholine receptors and acts on intestinal L-type calcium channels. 5-Hydroxyindole slows down the desensitization of 5-HT₃ receptor-mediated ion currents in cells. 5-Hydroxyindole causes convulsions and loss of consciousness. 5-Hydroxyindole is used in the study of neuroblastoma, schizophrenia, and diseases related to intestinal motility disorders.

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Reinheit: 99.94%
CAS. Nr.: 1953-54-4
Formel: C₈H₇NO
Molecular Weight:133.15

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

5-Hydroxyindole Chemical Structure

CAS. Nr.: 1953-54-4

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target^[1]^[2]^[3]^[4]^[5]^[6]^[7]

Microbial Metabolite

L-type calcium channel

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

Alle Calcium Channel Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
B16	IC₅₀	13 μM Compound: 18	Antimelanogenic activity in mouse B16 cells assessed as inhibition of intracellular melanin accumulation after 2 days Antimelanogenic activity in mouse B16 cells assessed as inhibition of intracellular melanin accumulation after 2 days	[PMID: 19524439]
B16	IC₅₀	68 μM Compound: 18	Inhibition of catecholase activity of tyrosinase in mouse B16 cells assessed as dopachrome formation Inhibition of catecholase activity of tyrosinase in mouse B16 cells assessed as dopachrome formation	[PMID: 19524439]

In Vitro

5-Hydroxyindole (1 mM) enhances the Acetylcholine-evoked glutamate release in rat cerebellar slices^[2].
5-Hydroxyindole (1-10 mM) can increase the amplitude of the maximum ion current evoked by 5-hydroxytryptamine (HY-B1473A) in mouse N1E-115 neuroblastoma cells^[3].
5-Hydroxyindole (30-300 mM) increases the amplitude of the field excitatory postsynaptic potential in rat hippocampal slices^[4].
5-Hydroxyindole (100 μM; 30 min) significantly increases 5-HT release from RIN14B cells^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

5-Hydroxyindole (50-100 mg/kg; i.p.) can cause convulsions and loss of consciousness in male Wistar rats^[4].
5-Hydroxyindole (30 mg/kg; p.o.; daily; 11 days) significantly decreases the total gut transit time (TGTT) and increases the defecation frequency in wild-type Groningen (WTG) adult male rats^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Wistar rats (weight: 100-200 g, age: 3-4 weeks)^[4]
Dosage:

50, 100 mg/kg (dissolved in DMSO)
Administration:

Intraperitoneal injection
Result:

Caused convulsions and loss of consciousness.
Caused immobility at 50 mg/kg.
Caused clonic and tonic convulsions after a latency period of approximately 10 min, and the total duration of the convulsive activity was 15-20 min.

Chemical Information

CAS. Nr. 1953-54-4
Appearance Solid
Molecular Weight 133.15
Formel C₈H₇NO
SMILES

OC1=CC2=C(C=C1)NC=C2
Structure Classification
- Alkaloids
- Indole Alkaloids
- Phenols
- Monophenols
Initial Source
- Endogenous metabolite
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (375.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

7.5103 mL

37.5516 mL

75.1033 mL

5 mM

1.5021 mL

7.5103 mL

15.0207 mL

10 mM

0.7510 mL

3.7552 mL

7.5103 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (18.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (18.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Huang J, et al. Effects of neuroactive metabolites of the tryptophan pathway on working memory and cortical thickness in schizophrenia. Transl Psychiatry. 2021 Apr 1;11(1):198. [Content Brief]

[2]. Zwart R, et al. 5-Hydroxyindole potentiates human alpha 7 nicotinic receptor-mediated responses and enhances acetylcholine-induced glutamate release in cerebellar slices. Neuropharmacology. 2002 Sep;43(3):374-84. [Content Brief]

[3]. Van Hooft J A, et al. Allosteric potentiation of the 5-HT3 receptor-mediated ion current in N1E-115 neuroblastoma cells by 5-hydroxyindole and analogues. Neuropharmacology, 1997, 36(4-5): 649-653.

[4]. Mannaioni G, et al. 5-hydroxyindole causes convulsions and increases transmitter release in the CA1 region of the rat hippocampus. Br J Pharmacol. 2003 Jan;138(1):245-53. [Content Brief]

[5]. Waclawiková B, et al. Gut bacteria-derived 5-hydroxyindole is a potent stimulant of intestinal motility via its action on L-type calcium channels. PLoS Biol. 2021 Jan 22;19(1):e3001070. [Content Brief]

[6]. Kooyman AR, et, al. 5-Hydroxyindole slows desensitization of the 5-HT3 receptor-mediated ion current in N1E-115 neuroblastoma cells. Br J Pharmacol. 1993 Feb;108(2):287-9. [Content Brief]

[7]. LaPorte M, et, al. 5-Hydroxyindoles by intramolecular alkynol-furan diels-alder cycloaddition. J Org Chem. 2013 Jan 4;78(1):167-74. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	7.5103 mL	37.5516 mL	75.1033 mL	187.7582 mL
	5 mM	1.5021 mL	7.5103 mL	15.0207 mL	37.5516 mL
	10 mM	0.7510 mL	3.7552 mL	7.5103 mL	18.7758 mL
	15 mM	0.5007 mL	2.5034 mL	5.0069 mL	12.5172 mL
	20 mM	0.3755 mL	1.8776 mL	3.7552 mL	9.3879 mL
	25 mM	0.3004 mL	1.5021 mL	3.0041 mL	7.5103 mL
	30 mM	0.2503 mL	1.2517 mL	2.5034 mL	6.2586 mL
	40 mM	0.1878 mL	0.9388 mL	1.8776 mL	4.6940 mL
	50 mM	0.1502 mL	0.7510 mL	1.5021 mL	3.7552 mL
	60 mM	0.1252 mL	0.6259 mL	1.2517 mL	3.1293 mL
	80 mM	0.0939 mL	0.4694 mL	0.9388 mL	2.3470 mL
	100 mM	0.0751 mL	0.3755 mL	0.7510 mL	1.8776 mL

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5-Hydroxyindole

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Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

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Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

5-Hydroxyindole Related Classifications

HY-W001160 Related Classifications