Art. -Nr.: HY-103269

BAI1

12 Cited Publications

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Based on 12 publication(s) in Google Scholar

BAI1 is a selective and allosteric inhibitor of BAX, an apoptosis regulator. BAI1 directly binds to BAX and allosterically inhibits BAX activation. BAI1 has the potential for the research of diseases mediated by BAX-dependent cell death.

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Reinheit: 99.68%
CAS. Nr.: 335165-68-9
Formel: C₁₉H₂₁Br₂N₃O
Molecular Weight:467.20

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

BAI1 Chemical Structure

CAS. Nr.: 335165-68-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (12)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target^[1]

Bax

Alle Bcl-2 Family Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	0.52 μM Compound: 1	In vitro inhibition of Bax regulated release of cytochrome C was determined using liposome channel assay In vitro inhibition of Bax regulated release of cytochrome C was determined using liposome channel assay	[PMID: 14521400]

In Vitro

BAI1 selectively inhibits BAX-mediated apoptotic cell death^[1].
? BAI1 shows inhibition of tBID (HY-100464)-induced BAX-mediated membrane permeabilization in a dose dependent manner with an IC₅₀ of 3.3 μΜ^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

Mouse MEF
Concentration:

0.3125 μM , 0.625 μM, 1 μM, 1.25 μM, 1.5 μM, 2.5 μM , 3 μM, 4 μM, 5 4 μM, 10 4 μM
Incubation Time:

8 hours
Result:

Inhibited cell death in BAX-dependent manner.

Chemical Information

CAS. Nr. 335165-68-9
Appearance Solid
Molecular Weight 467.20
Formel C₁₉H₂₁Br₂N₃O
Color White to off-white
SMILES

OC(CN1CCNCC1)CN2C3=C(C4=C2C=CC(Br)=C4)C=C(Br)C=C3
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (12)

All IFBio/Physico-chemical AssayRT-PCRHistological Imaging/StainingFlow Cytometry

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Journal Impact Factor

Adv Funct Mater

2023 Apr 28.
Nat Commun

A multifaceted strategy for intra- and extracellular nucleic acid regulation to alleviate intervertebral disc degeneration. [Abstract]

2025 Aug 26;16(1):7936. PMID: 40858573

BAI1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Aug 26;16(1):7936. [Abstract]

Immunofluorescence staining and quantification of BAX6A7 and dsDNA in aging NP cells treated with BAI1 (2.5 μM, 24 h), BNPM, or BNPMT.
Nat Commun

DLK-dependent axonal mitochondrial fission drives degeneration after axotomy. [Abstract]

2024 Dec 30;15(1):10806. PMID: 39737939
Nat Commun

Apoptotic dysregulation mediates stem cell competition and tissue regeneration. [Abstract]

2023 Nov 20;14(1):7547. PMID: 37985759
Mater Today Bio

Cyclic mechanical stretch suppresses intrinsic apoptosis in high metastatic melanoma. [Abstract]

2026 Apr 22:38:103132. PMID: 42111771
Cell Mol Life Sci

Dietary advanced glycation end-products exacerbate sarcopenia onset by activating apoptosis through PRMT1-mediated CRTC3 arginine methylation. [Abstract]

2025 Apr 7;82(1):142. PMID: 40192801

BAI1 purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2025 Apr 7;82(1):142. [Abstract]

Levels of free and protein-bound CML were significantly increased in both the BAI1 (50 mg/kg, i.p.)-treated and HAGE groups compared to the LAGE group.

BAI1 purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2025 Apr 7;82(1):142. [Abstract]

Histological analysis of skeletal muscle tissues demonstrated that BAI1 (50 mg/kg, i.p.) treatment significantly increased muscle fiber size and decreased connective tissue accumulation compared to the HAGE group.
Biochem Pharmacol

Targeting STING alleviates pyroptosis of bladder epithelial cells and ameliorates bladder fibrosis in neurogenic bladder. [Abstract]

2025 Jul 17:117177. PMID: 40683470
Int J Mol Sci

Mitochondrial Trafficking of MLKL, Bak/Bax, and Drp1 Is Mediated by RIP1 and ROS which Leads to Decreased Mitochondrial Membrane Integrity during the Hyperglycemic Shift to Necroptosis. [Abstract]

2023 May 11;24(10):8609. PMID: 37239951
Int Immunopharmacol

BAX pores facilitate mitochondrial DNA release in wasp sting-induced acute kidney injury. [Abstract]

2024 Oct 21;143(Pt 2):113424. PMID: 39437488
Ren Fail

Piezo1 facilitates the initiation and progression of renal fibrosis by mediating cell apoptosis and mitochondrial dysfunction. [Abstract]

2024 Dec;46(2):2415519. PMID: 39496543

BAI1 purchased from MedChemExpress. Usage Cited in: Ren Fail. 2024 Dec;46(2):2415519. [Abstract]

qPCR results indicated that Bax, Cytc, Caspase3, Cleaved-caspase3, KIM1, Fn and Collagen I expression were higher in the Yoda1 treatment group than in the DMSO group but lower in the Yoda1 + BAI1 (4 μM) group than in Yoda1 group.

BAI1 purchased from MedChemExpress. Usage Cited in: Ren Fail. 2024 Dec;46(2):2415519. [Abstract]

Flow cytometry scatter plot of JC1 staining after Yoda1 or/and BAI1 (4 μM) co-treatment, the FITC+ cells present the decreased of mitochondrial membrane potential.
Nat Prod Commun

2025 Apr 29.
University of Rijeka

2021 Jun.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 18.46 mg/mL (39.51 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1404 mL

10.7021 mL

21.4041 mL

5 mM

0.4281 mL

2.1404 mL

4.2808 mL

10 mM

0.2140 mL

1.0702 mL

2.1404 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1.87 mg/mL (4.00 mM); Clear solution

This protocol yields a clear solution of ≥ 1.87 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (18.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1.87 mg/mL (4.00 mM); Clear solution

This protocol yields a clear solution of ≥ 1.87 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (18.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Garner TP, et al. Small-molecule allosteric inhibitors of BAX. Nat Chem Biol. 2019 Apr;15(4):322-330. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1404 mL	10.7021 mL	21.4041 mL	53.5103 mL
	5 mM	0.4281 mL	2.1404 mL	4.2808 mL	10.7021 mL
	10 mM	0.2140 mL	1.0702 mL	2.1404 mL	5.3510 mL
	15 mM	0.1427 mL	0.7135 mL	1.4269 mL	3.5674 mL
	20 mM	0.1070 mL	0.5351 mL	1.0702 mL	2.6755 mL
	25 mM	0.0856 mL	0.4281 mL	0.8562 mL	2.1404 mL
	30 mM	0.0713 mL	0.3567 mL	0.7135 mL	1.7837 mL

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BAI1

Anti-Infection Compound Library

Apoptosis Compound Library

Anti-Cancer Compound Library

Anti-Blood Cancer Compound Library

Mitochondria-Targeted Compound Library

Targeted Diversity Library

Mitochondrial Protection Compound Library

Highly Selective Inhibitors Library

Cell Death Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Mitophagy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) BAI1

Alle Bcl-2 Family Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (12)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

BAI1

Anti-Infection Compound Library

Apoptosis Compound Library

Anti-Cancer Compound Library

Anti-Blood Cancer Compound Library

Mitochondria-Targeted Compound Library

Targeted Diversity Library

Mitochondrial Protection Compound Library

Highly Selective Inhibitors Library

Cell Death Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Mitophagy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) BAI1

Alle Bcl-2 Family Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (12)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

BAI1 Related Classifications

HY-103269 Related Classifications