CC-3060

CC-3060 (0.0001-10 μM; 6-16 h) potently and efficaciously induces CRBN- and proteasome-dependent degradation of wild-type ZBTB16 in HT-1080 ZBTB16-ePL cells, with a DC₅₀ of 0.47 nM and a minimum remaining protein level of 28%^[1].
CC-3060 (100 pM-10 μM; 6 h) drives CRBN- and proteasome-dependent degradation of endogenous ZBTB16 in HEL 92.1.7 cells, with complete degradation observed at 10 μM after 6 h^[1].
CC-3060 (10 μM; 6 h) induces selective degradation of ZBTB16, IKZF1, ZFP91, and ZNF276 (but not GSPT1 or CSNK1A1) in KG-1a cells, with a significant log2 fold change of −1.73 for ZBTB16^[1].
CC-3060 promotes CRL4^CRBN-dependent ubiquitination of ZBTB16 ZnF1 and ZnF3 domains in vitro, with activity dependent on the critical glycine residues in each domain^[1].
CC-3060 (0.0001-10 μM; 16 h) primarily utilizes the ZnF1 structural degron to promote ZBTB16 degradation in HT-1080 cells, with ZnF3 acting as a secondary, less efficient degron^[1].
CC-3060 (0.0001-10 μM; 6-16 h) potently induces CRBN- and proteasome-dependent degradation of ZBTB16-RARα in HT-1080 cells via the ZnF1 structural degron, with a minimum remaining protein level of 20%^[1].
CC-3060 stimulates CRL4^CRBN-dependent ubiquitination of ZBTB16-RARα in vitro, with activity dependent on the critical glycine residue in the ZBTB16 ZnF1 domain^[1].
CC-3060 (0.0001-10 μM; 6-16 h) induces CRBN- and proteasome-dependent degradation of RARα-ZBTB16 in HT-1080 cells via the ZnF3 structural degron, with a minimum remaining protein level of 77%^[1].
CC-3060 stimulates CRL4^CRBN-dependent ubiquitination of RARα-ZBTB16 in vitro, with activity dependent on the critical glycine residue in the ZBTB16 ZnF3 domain^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.