Seselin
Based on 1 Customer Validation
Seselin is an anticancer, antinociceptive, anti-inflammatory and antifungal agent. Seselin is orally active.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.55%
- CAS. Nr.: 523-59-1
- Formel: C14H12O3
- Molecular Weight:228.24
-
Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
-
Biologische Aktivität
-
Chemical Information
-
Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
-
Help & FAQs
Biologische Aktivität
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
>20 μg/mL
Compound: 16
|
Cytotoxicity against human A549 cells by MTT assay
Cytotoxicity against human A549 cells by MTT assay
|
[PMID: 29975532] |
| HepG2 | IC50 |
>20 μg/mL
Compound: 16
|
Cytotoxicity against human HepG2 cells by MTT assay
Cytotoxicity against human HepG2 cells by MTT assay
|
[PMID: 29975532] |
| MDA-MB-231 | IC50 |
>20 μg/mL
Compound: 16
|
Cytotoxicity against human MDA-MB-231 cells by MTT assay
Cytotoxicity against human MDA-MB-231 cells by MTT assay
|
[PMID: 29975532] |
| Neutrophil | IC50 |
>10 μM
Compound: 16
|
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release preincubated for 5 mins followed by fMLP/CB stimulation and measured for 10 mins using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as substrate
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release preincubated for 5 mins followed by fMLP/CB stimulation and measured for 10 mins using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as substrate
|
[PMID: 29975532] |
| Neutrophil | IC50 |
4.7 μM
Compound: 16
|
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation preincubated for 5 mins followed by cytochalasin B/fMLP addition for 3 mins by superoxide dismutase inhibitable reduction of ferricytochro
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation preincubated for 5 mins followed by cytochalasin B/fMLP addition for 3 mins by superoxide dismutase inhibitable reduction of ferricytochro
|
[PMID: 29975532] |
| Vero | IC50 |
>50 μg/mL
Compound: 4
|
Cytotoxicity against african green monkey Vero cells
Cytotoxicity against african green monkey Vero cells
|
[PMID: 21985060] |
| Vero | IC50 |
12 μg/mL
Compound: 10
|
Cytotoxicity against african green monkey Vero cells after 7 days by XTT assay
Cytotoxicity against african green monkey Vero cells after 7 days by XTT assay
|
[PMID: 8021652] |
Seselin shows cytotoxic effects with ED50 of 8.66 and 9.94 μg/mL against P-388 and HT-29 cells, respectively[1].
Seselin (5-20 μM; 0.5-24 h) inhibits cytokine output from macrophages stimulated by LPS and IFN-γ dose- and time-dependently[3].
Seselin (5-20 μM; 12 h) inhibits the expression of proinflammatory macrophage markers (iNOS, phagocytosis, CD11c) in BMDMs[3].
Seselin (5-20 μM; 0.5-6 h) blocks the STAT1 signalling pathway[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:Bone marrow-derived macrophages (BMDMs)
-
Concentration:5, 10 and 20 μM
-
Incubation Time:6 h
-
Result:Reduced the mRNA for cytokines (IL-1β, IL-6, Tnf-α and IL-23) and chemokines (Ccl3, Ccl7, Cxcl9 and Cxcl11) concentration-dependently in BMDMs.
-
Cell Line:BMDMs
-
Concentration:5, 10 and 20 μM
-
Incubation Time:0.5, 1.5, 3 and 6 h
-
Result:Suppressed expression of p-STAT1 and p-p65 both concentration and time dependently.
Seselin (3-30 mg/kg; i.g.; once) ameliorates sepsis induced by caecal ligation and puncture in mice[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Male Swiss mice[2]
-
Dosage:0.5, 4.5 or 40.5 mg/kg
-
Administration:Subcutaneous injection; once
-
Result:Inhibited the writhing response induced by acetic acid in a significant and dose-dependent manner, by 19.5%, 26.2% and 41.4% at dose of 0.5, 4.5 or 40.5 mg/kg, respectively. Elicited a significant inhibition of formalin response during the second phase (inflammatory), by 90.3%, 97.8% and 95.3%, respectively.
-
Animal Model:C57BL/6 mice, caecal ligation and puncture (CLP) induced sepsis model[3]
-
Dosage:3, 10 and 30 mg/kg
-
Administration:Intragastric administration, once
-
Result:Ameliorated lung injury and decreased JAK2 phosphorylation level in lung tissue during sepsis. Reduced the immune cell counts in BALF induced by CLP.
Chemical Information
-
CAS. Nr. 523-59-1
-
Appearance Solid
-
Molecular Weight 228.24
-
Formel C14H12O3
-
Color White to light yellow
-
SMILES
CC1(C=CC2=C(O1)C=CC3=C2OC(=O)C=C3)C
-
Structure Classification
-
Versand
Room temperature in continental US; may vary elsewhere.
-
Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
DMSO : 100 mg/mL (438.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.95 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.95 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
-
Data Sheet (281 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
Verweise
[1]. Chou HC, et al. Cytotoxic and anti-platelet aggregation constituents from the root wood of Melicope semecarpifolia. Planta Med. 2005 Nov;71(11):1078-81. [Content Brief]
[2]. Lima V, et al. Antinociceptive activity of the pyranocoumarin seselin in mice. Fitoterapia. 2006 Dec;77(7-8):574-8.Lima V, et al. Antinociceptive activity of the pyranocoumarin seselin in mice. Fitoterapia. 2006 Dec;77(7-8):574-8.Lima V, et al. Antinociceptive activity of the pyranocoumarin seselin in mice. Fitoterapia. 2006 Dec;77(7-8):574-8. [Content Brief]
[3]. Feng L, et al. Seselin ameliorates inflammation via targeting Jak2 to suppress the proinflammatory phenotype of macrophages. Br J Pharmacol. 2019 Jan;176(2):317-333. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.3814 mL | 21.9068 mL | 43.8135 mL | 109.5338 mL |
| 5 mM | 0.8763 mL | 4.3814 mL | 8.7627 mL | 21.9068 mL | |
| 10 mM | 0.4381 mL | 2.1907 mL | 4.3814 mL | 10.9534 mL | |
| 15 mM | 0.2921 mL | 1.4605 mL | 2.9209 mL | 7.3023 mL | |
| 20 mM | 0.2191 mL | 1.0953 mL | 2.1907 mL | 5.4767 mL | |
| 25 mM | 0.1753 mL | 0.8763 mL | 1.7525 mL | 4.3814 mL | |
| 30 mM | 0.1460 mL | 0.7302 mL | 1.4605 mL | 3.6511 mL | |
| 40 mM | 0.1095 mL | 0.5477 mL | 1.0953 mL | 2.7383 mL | |
| 50 mM | 0.0876 mL | 0.4381 mL | 0.8763 mL | 2.1907 mL | |
| 60 mM | 0.0730 mL | 0.3651 mL | 0.7302 mL | 1.8256 mL | |
| 80 mM | 0.0548 mL | 0.2738 mL | 0.5477 mL | 1.3692 mL | |
| 100 mM | 0.0438 mL | 0.2191 mL | 0.4381 mL | 1.0953 mL |
Powered by Bioz