Bisindolylmaleimide IV
Based on 1 Customer Validation
Bisindolylmaleimide IV (Arcyriarubin A) is a potent protein kinase C (PKC) inhibitor, with IC50s ranging from 0.1 to 0.55 μM. Bisindolylmaleimide IV also inhibits PKA (IC50=3.1-11.8μM). Bisindolylmaleimide IV is a potent, selective inhibitor of human cytomegalovirus (HCMV) replication in cell culture with an IC50 of 0.2 μM.
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- Pureté : 99.71%
- CAS No.: 119139-23-0
- Formule: C20H13N3O2
- Masse moléculaire:327.34
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Stockage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Bisindolylmaleimide IV (Standard)PKC/HCMV Inhibitor
Activité biologique
Description
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PKC 0.1-0.55 μM (IC50) |
HCMV 0.2 μM (IC50) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| A549 | IC50 |
4.14 μM
Compound: Bisindolyl-maleimide
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Cytotoxicity activity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
Cytotoxicity activity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
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[PMID: 30119011] |
| A549 | IC50 |
56.61 μM
Compound: Ro-316233
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Antiproliferative activity against human A549 cells after 48 hrs by MTT assay
Antiproliferative activity against human A549 cells after 48 hrs by MTT assay
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[PMID: 19447039] |
| ECa-109 cell line | IC50 |
15.9 μM
Compound: Bisindolyl-maleimide
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Cytotoxicity activity against human Eca-109 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
Cytotoxicity activity against human Eca-109 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
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[PMID: 30119011] |
| ECa-109 cell line | IC50 |
83.55 μM
Compound: Ro-316233
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Antiproliferative activity against human ECA109 cells after 48 hrs by MTT assay
Antiproliferative activity against human ECA109 cells after 48 hrs by MTT assay
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[PMID: 19447039] |
| HL-60 | CC50 |
>8 μM
Compound: 82
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Cytotoxicity against human HL-60 cells
Cytotoxicity against human HL-60 cells
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[PMID: 33539089] |
| Jurkat | IC50 |
6.3 μg/mL
Compound: 4
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Cytotoxicity against Jurkat cells
Cytotoxicity against Jurkat cells
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[PMID: 15911254] |
| K562 | IC50 |
17.46 μM
Compound: Ro-316233
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Antiproliferative activity against human K562 cells after 48 hrs by MTT assay
Antiproliferative activity against human K562 cells after 48 hrs by MTT assay
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[PMID: 19447039] |
| KB | IC50 |
53.78 μM
Compound: Ro-316233
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Antiproliferative activity against human KB cells after 48 hrs by MTT assay
Antiproliferative activity against human KB cells after 48 hrs by MTT assay
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[PMID: 19447039] |
| KB 3-1 | IC50 |
>25 μg/mL
Compound: 4
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Cytotoxicity against KB/VJ300 cells
Cytotoxicity against KB/VJ300 cells
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[PMID: 15911254] |
| MCF7 | CC50 |
>8 μM
Compound: 82
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Cytotoxicity against human MCF7 cells
Cytotoxicity against human MCF7 cells
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[PMID: 33539089] |
| PC-3 | IC50 |
10.3 μM
Compound: Bisindolyl-maleimide
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Cytotoxicity activity against human PC-3 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
Cytotoxicity activity against human PC-3 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
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[PMID: 30119011] |
| SMMC-7721 | IC50 |
>100 μM
Compound: Ro-316233
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Antiproliferative activity against human SMMC7721 cells after 48 hrs by MTT assay
Antiproliferative activity against human SMMC7721 cells after 48 hrs by MTT assay
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[PMID: 19447039] |
| SMMC-7721 | IC50 |
13.2 μM
Compound: Bisindolyl-maleimide
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Cytotoxicity activity against human SMMC-7721 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
Cytotoxicity activity against human SMMC-7721 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
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[PMID: 30119011] |
In Vitro
Chemical Information
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CAS No. 119139-23-0
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Appearance Solid
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Masse moléculaire 327.34
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Formule C20H13N3O2
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Color Orange to red
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SMILES
O=C(C(C1=CNC2=C1C=CC=C2)=C3C4=CNC5=C4C=CC=C5)NC3=O
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Synonyms
Arcyriarubin A
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
DMSO : 125 mg/mL (381.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.35 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocole
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Directly Induced Neuron Culture
Directly induced neuron culture converts somatic cells, most commonly fibroblasts, into induced neurons without passing through a pluripotent or neural progenitor stage; classic evidence shows that mouse fibroblasts can be converted by Ascl1, Brn2/Pou3f2, and Myt1l, human fibroblasts can be converted by defined neuronal transcription factors, and human fibroblasts can also be converted by miR-9/9-124 with neurogenic or subtype-specifying transcription factors. The readout is acquisition of neuronal identity and function, assessed by neuronal morphology, neuronal markers such as Tuj1/βIII-tubulin, MAP2, synapsin, and subtype markers when relevant, together with functional assays such as action-potential firing, synaptic activity, and electrophysiology.
Pureté et documentation
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Fiche technique (278 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.0549 mL | 15.2746 mL | 30.5493 mL | 76.3732 mL |
| 5 mM | 0.6110 mL | 3.0549 mL | 6.1099 mL | 15.2746 mL | |
| 10 mM | 0.3055 mL | 1.5275 mL | 3.0549 mL | 7.6373 mL | |
| 15 mM | 0.2037 mL | 1.0183 mL | 2.0366 mL | 5.0915 mL | |
| 20 mM | 0.1527 mL | 0.7637 mL | 1.5275 mL | 3.8187 mL | |
| 25 mM | 0.1222 mL | 0.6110 mL | 1.2220 mL | 3.0549 mL | |
| 30 mM | 0.1018 mL | 0.5092 mL | 1.0183 mL | 2.5458 mL | |
| 40 mM | 0.0764 mL | 0.3819 mL | 0.7637 mL | 1.9093 mL | |
| 50 mM | 0.0611 mL | 0.3055 mL | 0.6110 mL | 1.5275 mL | |
| 60 mM | 0.0509 mL | 0.2546 mL | 0.5092 mL | 1.2729 mL | |
| 80 mM | 0.0382 mL | 0.1909 mL | 0.3819 mL | 0.9547 mL | |
| 100 mM | 0.0305 mL | 0.1527 mL | 0.3055 mL | 0.7637 mL |
Bisindolylmaleimide IV Related Classifications
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