1. Metabolic Enzyme/Protease
  2. Phospholipase
  3. Bromoenol lactone

Bromoenol lactone (Synonyms: (6E)-Bromoenol lactone)

Cat. No.: HY-107411 Purity: >98.0%
Handling Instructions

Bromoenol lactone ((6E)-Bromoenol lactone) is a suicide-based irreversible, selective, potent inhibitor of calcium-independent phospholipase A2 (iPLA2β) with an IC50 value of approximately 7 μM, which inhibits antigen-stimulated mast cell exocytosis without blocking Ca2+ influx.

For research use only. We do not sell to patients.

Bromoenol lactone Chemical Structure

Bromoenol lactone Chemical Structure

CAS No. : 88070-98-8

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Description

Bromoenol lactone ((6E)-Bromoenol lactone) is a suicide-based irreversible, selective, potent inhibitor of calcium-independent phospholipase A2 (iPLA2β) with an IC50 value of approximately 7 μM, which inhibits antigen-stimulated mast cell exocytosis without blocking Ca2+ influx[1][2].

IC50 & Target[1][2]

PLA2

7 μM (IC50)

In Vitro

In RBL 2H3 and bone marrow-derived mast cells (BMMCs), Ca2+ entry is critical for exocytosis. Bromoenol lactone inhibits exocytosis when stimulated using a Ca2+ ionophore A23187, which passively transports Ca2+ down a concentration gradient and also in permeabilised mast cells where Ca2+ entry is no longer relevant. Moreover, Bromoenol lactone has only a minor effect on antigen- or thapsigargin-stimulated Ca2+ signalling, both the release from internal stores and sustained elevation due to Ca2+ influx[1].

Molecular Weight

317.18

Formula

C₁₆H₁₃BrO₂

CAS No.

88070-98-8

SMILES

O=C1C(C2=C3C=CC=CC3=CC=C2)CC/C(O1)=C\Br

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Pure form -20°C 3 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (788.20 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1528 mL 15.7639 mL 31.5278 mL
5 mM 0.6306 mL 3.1528 mL 6.3056 mL
10 mM 0.3153 mL 1.5764 mL 3.1528 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 6.25 mg/mL (19.70 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

Bromoenol lactone(6E)-Bromoenol lactonePhospholipaseInhibitorinhibitorinhibit

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Product Name:
Bromoenol lactone
Cat. No.:
HY-107411
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