Carvacrol (Synonyms: Cymophenol)

Name: Carvacrol
Brand: MedChemExpress
SKU: HY-N0711
Rating: 5.0 (10 reviews)

Cat. No.: HY-N0711 Purity: 99.96%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Carvacrol is an orally active and blood-brain barrier-permeable monoterpenic phenol that can be extract from an abundant number of aromatic plants, including thyme and oregano, possessing antioxidant, antibacterial, antifungal, anticancer, anti-inflammatory, hepatoprotective, spasmolytic, and vasorelaxant properties. Carvacrol also causes cell cycle arrest in G0/G1, downregulates Notch-1, and Jagged-1, and induces apoptosis. Carvacrol is used in low concentrations as a food flavoring ingredient and preservative, as well as a fragrance ingredient in cosmetic formulations.

For research use only. We do not sell to patients.

CAS No. : 499-75-2

Size	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Liquid
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 10 publication(s) in Google Scholar

Other Forms of Carvacrol:

Carvacrol (Standard) In-stock

10 Publications Citing Use of MCE Carvacrol

Cell Imaging/Staining

In Vivo Efficacy Study

Histological Imaging/Staining

IHC

RT-PCR

: Carvacrol purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2025 Sep 29:16:1671461. [Abstract]; Representative images of DRG neurons in response to 100 μM Carvacrol and 100 μM AITC after pretreatment with vehicle, DMI (100 μM), or DMI + HC-030031 (100 μM).

: Carvacrol purchased from MedChemExpress. Usage Cited in: Transl Oncol. 2025 May 26:58:102431. [Abstract]; The shortening of colon length, indicative of colitis severity induced by DSS, was significantly alleviated in the Carvacrol group.

: Carvacrol purchased from MedChemExpress. Usage Cited in: Transl Oncol. 2025 May 26:58:102431. [Abstract]; Carvacrol markedly reduced the histopathological damage in DSS-induced colitis.

: Carvacrol purchased from MedChemExpress. Usage Cited in: Transl Oncol. 2025 May 26:58:102431. [Abstract]; Tissue immunohistochemistry results showed a reduction in MUC2 expression in DSS-induced colitis mice, which was significantly restored by Carvacrol treatment.

: Carvacrol purchased from MedChemExpress. Usage Cited in: Transl Oncol. 2025 May 26:58:102431. [Abstract]; qPCR analysis revealed that the expression levels of ZO-1, Claudin3, Claudin1, Occludin, and Mucin were significantly increased in the Carvacrol group compared to the DSS group.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	17 μM Compound: Carvacrol	Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by Fluo-4-AM dye based fluorescence assay Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by Fluo-4-AM dye based fluorescence assay	[PMID: 30878828]
HEK293	EC₅₀	7 μM Compound: 24	Agonist activity at human TRPA1 channel expressed in HEK293 cells assessed as increase in intracellular calcium influx Agonist activity at human TRPA1 channel expressed in HEK293 cells assessed as increase in intracellular calcium influx	[PMID: 20356305]

In Vivo

Carvacrol (50 or 100 mg/kg; i.p.) inhibits the mechanical hyper-nociception induced by carrageenan in mice^[2].
Carvacrol (20, 40, and 80 mg/kg, intragastric gavage, during six days before LPS injection) reduces inflammation (reduces serum proinflammatory molecules) in rats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

150.22

Formula

C₁₀H₁₄O

CAS No.

499-75-2

Appearance

Liquid (Density: 0.976 g/cm³)

Color

Colorless to light yellow

SMILES

OC1=CC(C(C)C)=CC=C1C

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (665.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.6569 mL	33.2845 mL	66.5690 mL
5 mM	1.3314 mL	6.6569 mL	13.3138 mL
10 mM	0.6657 mL	3.3285 mL	6.6569 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (16.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (16.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (16.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.98%

Select Batch:

Data Sheet (273 KB) SDS (759 KB)

COA (246 KB) HNMR (131 KB) GC (243 KB)

Handling Instructions (2659 KB)

References

[1]. Khan F, et al. Carvacrol Induced Program Cell Death and Cell Cycle Arrest in Androgen-Independent Human Prostate Cancer Cells via Inhibition of Notch Signaling. Anticancer Agents Med Chem. 2019 Jul 31. [Content Brief]

[2]. Suntres ZE, et al. The bioactivity and toxicological actions of carvacrol. Crit Rev Food Sci Nutr. 2015;55(3):304-18. [Content Brief]

[3]. Kara M, et al. Supplemental carvacrol can reduce the severity of inflammation by influencing the production of mediators of inflammation. Inflammation. 2015;38(3):1020-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.6569 mL	33.2845 mL	66.5690 mL	166.4226 mL
	5 mM	1.3314 mL	6.6569 mL	13.3138 mL	33.2845 mL
	10 mM	0.6657 mL	3.3285 mL	6.6569 mL	16.6423 mL
	15 mM	0.4438 mL	2.2190 mL	4.4379 mL	11.0948 mL
	20 mM	0.3328 mL	1.6642 mL	3.3285 mL	8.3211 mL
	25 mM	0.2663 mL	1.3314 mL	2.6628 mL	6.6569 mL
	30 mM	0.2219 mL	1.1095 mL	2.2190 mL	5.5474 mL
	40 mM	0.1664 mL	0.8321 mL	1.6642 mL	4.1606 mL
	50 mM	0.1331 mL	0.6657 mL	1.3314 mL	3.3285 mL
	60 mM	0.1109 mL	0.5547 mL	1.1095 mL	2.7737 mL
	80 mM	0.0832 mL	0.4161 mL	0.8321 mL	2.0803 mL
	100 mM	0.0666 mL	0.3328 mL	0.6657 mL	1.6642 mL

Carvacrol Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Carvacrol (Synonyms: Cymophenol)

Customer Review

Other Forms of Carvacrol:

Carvacrol Related Antibodies

10 Publications Citing Use of MCE Carvacrol

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Carvacrol Related Classifications

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Customer Review

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