Art. -Nr.: HY-101853

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STAT5-IN-1

32 Cited Publications

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Based on 32 publication(s) in Google Scholar

STAT5-IN-1 is a STAT5 inhibitor with an IC₅₀ of 47 μM for STAT5β isoform.

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Reinheit: 98.35%
CAS. Nr.: 285986-31-4
Formel: C₁₆H₁₁N₃O₃
Molecular Weight:293.28

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months

STAT5-IN-1 Chemical Structure

CAS. Nr.: 285986-31-4

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (32)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Bioz

Biologische Aktivität

Beschreibung

STAT5-IN-1 is a STAT5 inhibitor with an IC₅₀ of 47 μM for STAT5β isoform.

IC₅₀ & Target

STAT5β

47 μM (IC₅₀)

Alle STAT Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
Neutrophil	IC₅₀	>1000 μM Compound: 18	Antiinflammatory activity in human neutrophils assessed as inhibition of respiratory burst Antiinflammatory activity in human neutrophils assessed as inhibition of respiratory burst	[PMID: 20576329]

In Vitro

The signal transducer and activator of transcription 5 (STAT5) is a member of the STAT family of proteins, implicated in cell growth and differentiation. STAT5-IN-1 inhibits STAT5 by binding to the SH2 domain. The functions of the SH2 domains of STAT3, STAT1, and of the tyrosine kinase Lck are inhibited to a lesser extent (IC50>500 μM). STAT5-IN-1 block STAT5/STAT5 DNA binding in K562 nuclear extracts. Substitution of the hydrogen at C6 of the chromone ring by an ethyl group does not affect activity of STAT5-IN-1 against STAT5β, but leads to complete loss of selectivity against other STAT family members^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 285986-31-4
Appearance Solid
Molecular Weight 293.28
Formel C₁₆H₁₁N₃O₃
Color White to light yellow
SMILES

O=C(C1=CC=CN=C1)N/N=C/C2=COC3=CC=CC=C3C2=O
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (32)

All WBFlow CytometryApoptosis AnalysisRT-PCR

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Journal Impact Factor

Signal Transduct Target Ther

Macrophages promote pre-metastatic niche formation of breast cancer through aryl hydrocarbon receptor activity. [Abstract]

2024 Dec 18;9(1):352. PMID: 39690159

STAT5-IN-1 purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Dec 18;9(1):352. [Abstract]

Western blotting analysis of AHR expression in STAT5-IN-1 (1, 5, 10 μM)-treated peritoneal macrophages, with or without 4T1-CM treatment for 24 h.

STAT5-IN-1 purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Dec 18;9(1):352. [Abstract]

mRNA levels of Ahr in peritoneal macrophages treated with or without 4T1-CM, in the presence or absence of STAT5-IN-1 (1, 5, or 10 μM) for 24 h.
Cell Metab

Methionine-supplemented longevity diet increases growth hormone, GLP-1, and FGF21; reduces frailty; and promotes healthspan. [Abstract]

2026 Jun 23:S1550-4131(26)00225-1. PMID: 42335894
Cell Metab

Neutrophils resist ferroptosis and promote breast cancer metastasis through aconitate decarboxylase 1. [Abstract]

2023 Oct 3;35(10):1688-1703.e10. PMID: 37793345
Nat Immunol

Cell cycle arrest enhances CD8+ T cell effector function by potentiating glucose metabolism and IL-2 signaling. [Abstract]

2026 Mar;27(3):463-475. PMID: 41555086
Cell Mol Immunol

γδ+ T-cell-derived IL-17A stimulates airway epithelial/stromal cells to secrete G-CSF, promoting lung-specific pathogenic Siglec-F+ neutrophil development in PPE-induced emphysema. [Abstract]

2025 Jul;22(7):791-805. PMID: 40461699
Cancer Res

Inhibition of Ephrin B2 Reverse Signaling Suppresses Multiple Myeloma Pathogenesis. [Abstract]

2024 Mar 15;84(6):919-934. PMID: 38231476
Nat Commun

The E3 ligases Itch and WWP2 regulate autoimmune neuroinflammation by controlling TH2 to TH17 cell conversion via interleukin-4-STAT5 axis in mice. [Abstract]

2026 Jan 23;17(1):952. PMID: 41577662

STAT5-IN-1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2026 Jan 23;17(1):952. [Abstract]

Frequency of IL-4 and IL-17A production in CD4+ T cells from DKO and TKO mice cultured in vitro under TH0 to TH17 conditions with the treatment of STAT5-IN (1, 10, 50 μM).
Nat Commun

Osteocytes directly regulate osteolysis via MYD88 signaling in bacterial bone infection. [Abstract]

2022 Nov 4;13(1):6648. PMID: 36333322
Nat Commun

Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function. [Abstract]

2018 Nov 19;9(1):4874. PMID: 30451838
Biomaterials

Nrf-2-driven long noncoding RNA ODRUL contributes to modulating silver nanoparticle-induced effects on erythroid cells. [Abstract]

2017 Jun;130:14-27. PMID: 28351005
Cancer Lett

STAT5 is essential for inducing the suppressive subset and attenuate cytotoxicity of Vδ2+ T cells in acute myeloid leukemia. [Abstract]

2024 Apr 10:587:216730. PMID: 38360140
Proc Natl Acad Sci U S A

PDGF-D-PDGFRβ signaling enhances IL-15-mediated human natural killer cell survival. [Abstract]

2022 Jan 18;119(3):e2114134119. PMID: 35027451
Chemosphere

Cadmium impairs the development of natural killer cells and bidirectionally modifies their capacity for cytotoxicity. [Abstract]

2023 Jan;311(Pt 1):137068. PMID: 36330983
Mucosal Immunol

Extracellular vesicles of Bacteroides uniformis induce M1 macrophage polarization and aggravate gut inflammation during weaning. [Abstract]

2024 May 20:S1933-0219(24)00045-X. PMID: 38777177
Cell Mol Gastroenterol Hepatol

Oncostatin M induces IFITM1 expression to inhibit hepatitis B virus replication via JAK-STAT signaling. [Abstract]

2024;17(2):219-235. PMID: 37879404
Cell Death Discov

Ablation of Cbl-b and c-Cbl in dendritic cells causes spontaneous liver cirrhosis via altering multiple properties of CD103+ cDC1s. [Abstract]

2022 Mar 30;8(1):142. PMID: 35354799

STAT5-IN-1 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2022 Mar 30;8(1):142. [Abstract]

STAT5-IN-1 (25, 50 μM) was added to CD103⁺ cDC cultures at the indicated concentration for 9 days after which apoptosis levels were analyzed by flow cytometry.
Cell Rep

Blocking DCIR mitigates colitis and prevents colorectal tumors by enhancing the GM-CSF-STAT5 pathway. [Abstract]

2022 Aug 2;40(5):111158. PMID: 35926458
Sci Signal

Modulation of PKM activity affects the differentiation of TH17 cells. [Abstract]

2020 Oct 27;13(655):eaay9217. PMID: 33109748
J Agric Food Chem

miR-204-5p and miR-211 Synergistically Downregulate the αS1-Casein Content and Contribute to the Lower Allergy of Goat Milk. [Abstract]

2021 May 12;69(18):5353-5362. PMID: 33939400
JCI Insight

Single-cell profiling reveals pathogenic role and differentiation trajectory of granzyme K+ CD8+T cells in primary Sjögren's syndrome. [Abstract]

2023 Apr 24;8(8):e167490. PMID: 36881472
Hum Reprod

Decidua-derived granulocyte macrophage colony-stimulating factor induces polymorphonuclear myeloid-derived suppressor cells from circulating CD15+ neutrophils. [Abstract]

2020 Dec 1;35(12):2677-2691. PMID: 33067638
Front Immunol

IL-2 Inhibition of Th17 Generation Rather Than Induction of Treg Cells Is Impaired in Primary Sjögren's Syndrome Patients. [Abstract]

2018 Aug 13:9:1755. PMID: 30150979
Biochem Pharmacol

Janus kinase inhibitor ruxolitinib blocks thymic regeneration after acute thymus injury. [Abstract]

2020 Jan;171:113712. PMID: 31726048
Cells

Pathologic Th1-Treg Cells Exacerbate Acute Lung Injury and Lethality in Sepsis. [Abstract]

2026 Mar 14;15(6):521. PMID: 41892312
Fish Shellfish Immunol

Large yellow croaker (Lrimichthys crocea) IL-2 modulates humoral immunity via the conserved JAK-STAT5 signal pathway. [Abstract]

2023 Feb:133:108519. PMID: 36608811
Microbiol Spectr

Galectin-9 Facilitates Epstein-Barr Virus Latent Infection and Lymphomagenesis in Human B Cells. [Abstract]

2023 Feb 14;11(1):e0493222. PMID: 36622166
J Neuroimmune Pharmacol

Low-dose IL-2 Ameliorates Experimental Autoimmune Myasthenia Gravis in Rats by Restoring the CD4-positive Helper T-cell Balance Via the JAK/STAT5 Pathway. [Abstract]

2026 Jan 31;21(1):9. PMID: 41619069
J Immunol

IL-2 Signaling Couples the MAPK and mTORC1 Axes to Promote T Cell Proliferation and Differentiation in Teleosts. [Abstract]

2022 Apr 1;208(7):1616-1631. PMID: 35321881
J Leukoc Biol

STAT5 promotes chronic pancreatitis by enhancing GM-CSF-dependent neutrophil augmentation. [Abstract]

2021 Aug;110(2):293-300. PMID: 34184320
Cell Immunol

Regulation of ROS in myeloid-derived suppressor cells through targeting fatty acid transport protein 2 enhanced anti-PD-L1 tumor immunotherapy. [Abstract]

2021 Apr:362:104286. PMID: 33524739
Res Sq

2025 May 12.
Biol Blood Marrow Transplant

IL-22 Accelerates Thymus Regeneration via Stat3/Mcl-1 and Decreases Chronic Graft-versus-Host Disease in Mice after Allotransplants. [Abstract]

2019 Oct;25(10):1911-1919. PMID: 31195136

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 20 mg/mL (68.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.4097 mL

17.0486 mL

34.0971 mL

5 mM

0.6819 mL

3.4097 mL

6.8194 mL

10 mM

0.3410 mL

1.7049 mL

3.4097 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2 mg/mL (6.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline

Solubility: 12.5 mg/mL (42.62 mM); Suspended solution; Need ultrasonic

Protocol 2

Add each solvent one by one: 0.5% CMC-Na/saline water

Solubility: 12.5 mg/mL (42.62 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Müller J, et al. Discovery of chromone-based inhibitors of the transcription factor STAT5. Chembiochem. 2008 Mar 25;9(5):723-7. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.4097 mL	17.0486 mL	34.0971 mL	85.2428 mL
	5 mM	0.6819 mL	3.4097 mL	6.8194 mL	17.0486 mL
	10 mM	0.3410 mL	1.7049 mL	3.4097 mL	8.5243 mL
	15 mM	0.2273 mL	1.1366 mL	2.2731 mL	5.6829 mL
	20 mM	0.1705 mL	0.8524 mL	1.7049 mL	4.2621 mL
	25 mM	0.1364 mL	0.6819 mL	1.3639 mL	3.4097 mL
	30 mM	0.1137 mL	0.5683 mL	1.1366 mL	2.8414 mL
	40 mM	0.0852 mL	0.4262 mL	0.8524 mL	2.1311 mL
	50 mM	0.0682 mL	0.3410 mL	0.6819 mL	1.7049 mL
	60 mM	0.0568 mL	0.2841 mL	0.5683 mL	1.4207 mL

Keywords

STAT5-IN-1

32 Cited Publications

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STAT5-IN-1

Immunology/Inflammation Compound Library

JAK/STAT Compound Library

Kinase Inhibitor Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Obesity Compound Library

Transcription Factor-Targeted Library

Targeted Diversity Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Cysteine Targeted Covalent Library

Highly Selective Inhibitors Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) STAT5-IN-1

Alle STAT Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (32)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

STAT5-IN-1

Immunology/Inflammation Compound Library

JAK/STAT Compound Library

Kinase Inhibitor Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Obesity Compound Library

Transcription Factor-Targeted Library

Targeted Diversity Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Cysteine Targeted Covalent Library

Highly Selective Inhibitors Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) STAT5-IN-1

Alle STAT Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (32)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

STAT5-IN-1 Related Classifications

Art. -Nr.: HY-101853