Art. -Nr.: HY-15659

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Wnt-C59

(Synonyms: C59)

37 Cited Publications

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Based on 37 publication(s) in Google Scholar

Wnt-C59 (C59) is a highly potent and oral porcupine (PORCN) inhibitor with an IC₅₀ of 74 pM.

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Reinheit: 99.88%
CAS. Nr.: 1243243-89-1
Formel: C₂₅H₂₁N₃O
Molecular Weight:379.45

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Wnt-C59 Chemical Structure

CAS. Nr.: 1243243-89-1

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (37)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Protokoll
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Wnt-C59 (C59) is a highly potent and oral porcupine (PORCN) inhibitor with an IC₅₀ of 74 pM.

IC₅₀ & Target

IC50: 74 pM (PORCN)^[1]

Alle Wnt Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
HT-1080	IC₅₀	74 pM Compound: C59	Inhibition of porcupine activity (unknown origin) expressed in human HT1080 cells assessed as suppression of Wnt3A-mediated super top flash activity by STF luciferase assay Inhibition of porcupine activity (unknown origin) expressed in human HT1080 cells assessed as suppression of Wnt3A-mediated super top flash activity by STF luciferase assay	[PMID: 26522946]

In Vitro

Wnt-C59 (C59) inhibits PORCN activity in vitro at nanomolar concentrations, as assessed by inhibition of Wnt palmitoylation, Wnt interaction with the carrier protein Wntless/WLS, Wnt secretion, and Wnt activation of β-catenin reporter activity. Wnt-C59 inhibits WNT3A-mediated activation of a multimerized TCF-binding site driving luciferase with an IC₅₀ of 74 pM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Wnt-C59 displays good bioavailability in mice. Wnt-C59 blocks progression of mammary tumors in MMTV-WNT1 transgenic mice while downregulating Wnt/β-catenin target genes^[1].
Wnt-C59 has the potential to eradicate cancer stem cells in human tumors. Wnt-C59 inhibits stemness properties of NPC cells in a dosage-dependent manner by arresting sphere formation in both HNE1 and SUNE1 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 1243243-89-1
Appearance Solid
Molecular Weight 379.45
Formel C₂₅H₂₁N₃O
Color White to yellow
SMILES

O=C(NC1=CC=C(C2=CC=CN=C2)C=C1)CC3=CC=C(C4=CC(C)=NC=C4)C=C3
Synonyms

C59
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (37)

All Cell Proliferation/Viability Assay2D/3D Cell Culture and DifferentiationWBHistological Imaging/StainingRT-PCR

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Journal Impact Factor

Adv Sci (Weinh)

Preservation of ALYREF Phase Separation Mitigates Doxorubicin-Induced Cardiomyocyte DNA Damage and Cardiotoxicity. [Abstract]

2025 Sep 3:e05270. PMID: 40899520
Adv Sci (Weinh)

Therapy-Induced ECM Remodeling Creates a Transient Immune Barrier in Residual Melanoma. [Abstract]

2025 Aug 22:e08451. PMID: 40847444
Exp Mol Med

Switching off CK2-mediated activation of survivin offers new therapeutic opportunities in neuroblastoma. [Abstract]

2026 Feb;58(1):227-242. PMID: 41571889
J Exp Clin Cancer Res

Hedgehog signaling promotes sorafenib resistance in hepatocellular carcinoma patient-derived organoids. [Abstract]

2020 Jan 28;39(1):22. PMID: 31992334

Wnt-C59 purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2020 Jan 28;39(1):22. [Abstract]

4 HCC PDOs were treated with a Notch signaling inhibitor (YO-01027), Wnt signaling inhibitor (Wnt-C59, 0-20 μM), Hippo signaling inhibitor (verteporfin), or Hedgehog signaling inhibitor (GANT61) for 6 days and cell viability was recorded.
Gut Microbes

Fucose promotes intestinal stem cell-mediated intestinal epithelial development through promoting Akkermansia-related propanoate metabolism. [Abstract]

2023 Jan-Dec;15(1):2233149. PMID: 37424378

Wnt-C59 purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2023 Jan-Dec;15(1):2233149. [Abstract]

Images of organoids that were treated with ileal contents from control and fucose-treated mice when there were Wnt signaling inhibitor Wnt-c59 (100 nM).

Wnt-C59 purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2023 Jan-Dec;15(1):2233149. [Abstract]

Lgr5-EGFP staining of organoids that were treated with ileal contents from control and fucose-treated mice when there were Wnt signaling inhibitor Wnt-c59 (100 nM).

Wnt-C59 purchased from MedChemExpress. Usage Cited in: Gut Microbes. 2023 Jan-Dec;15(1):2233149. [Abstract]

EdU staining of organoids that were treated with ileal contents from control and fucose-treated mice when there were Wnt signaling inhibitor Wnt-c59 (100 nM).
Acta Pharmacol Sin

PLN-L31A/I40A for the treatment of inherited heart disease caused by PLN-R14del mutations. [Abstract]

2026 Jan 16. PMID: 41545752
Acta Pharmacol Sin

Inhibiting L-type calcium channel promotes cardiomyocyte proliferation through activating the canonical Wnt signaling pathway. [Abstract]

2026 Jan 7. PMID: 41501415
Stem Cell Res Ther

HAND factors regulate cardiac lineage commitment and differentiation from human pluripotent stem cells. [Abstract]

2024 Feb 5;15(1):31. PMID: 38317221
Cell Chem Biol

STT3A is essential for Wnt signaling and represents a target for cancers driven by RNF43 deficiency. [Abstract]

2025 Oct 22:S2451-9456(25)00306-X. PMID: 41130209
Cell Rep

Efficient exon skipping by base-editor-mediated abrogation of exonic splicing enhancers. [Abstract]

2023 Oct 30;42(11):113340. PMID: 37906593
Br J Cancer

Fibroblast growth factor signals drive the metastatic behavior in small cell lung cancer. [Abstract]

2025 Dec 13. PMID: 41390896
EMBO Rep

Noncanonical Wnt signaling promotes colon tumor growth, chemoresistance and tumor fibroblast activation. [Abstract]

2023 Apr 5;24(4):e54895. PMID: 36704936

Wnt-C59 purchased from MedChemExpress. Usage Cited in: EMBO Rep. 2023 Apr 5;24(4):e54895. [Abstract]

LAKTP murine tumor (MTO) cells were seeded in the indicated culture conditions and supplemented with LGK (10 μM) or WntC59 (100 nM). Total protein extracts were obtained and analyzed after 16 h of treatment.

Wnt-C59 purchased from MedChemExpress. Usage Cited in: EMBO Rep. 2023 Apr 5;24(4):e54895. [Abstract]

LAKTP murine tumor (MTO) cells were seeded in the indicated culture conditions and supplemented with LGK (10 μM) or WntC59 (100 nM). Total RNA extracts were obtained and analyzed after 16 h of treatment.

Wnt-C59 purchased from MedChemExpress. Usage Cited in: EMBO Rep. 2023 Apr 5;24(4):e54895. [Abstract]

Cell number was analyzed by CellTiter‐Go assay in MTO cells supplemented with WntC59 (100 nM) every 3 days (mean ± SD of three biological replicates).
JCI Insight

WNT signaling contributes to the extrahepatic bile duct proliferative response to obstruction in mice. [Abstract]

2024 Dec 5:e181857. PMID: 39636699
Mol Ther Nucleic Acids

ALKBH5-mediated m6A mRNA methylation governs human embryonic stem cell cardiac commitment. [Abstract]

2021 May 29:26:22-33. PMID: 34513291
Front Immunol

Wnt Inhibition Sensitizes PD-L1 Blockade Therapy by Overcoming Bone Marrow-Derived Myofibroblasts-Mediated Immune Resistance in Tumors. [Abstract]

2021 Mar 15:12:619209. PMID: 33790893
Int J Mol Med

MicroRNA‑381/Hes1 is a potential therapeutic target for spinal cord injury. [Abstract]

2018 Aug;42(2):1008-1017. PMID: 29750292
Int J Mol Sci

Unveiling the Antiviral Properties of Panduratin A through SARS-CoV-2 Infection Modeling in Cardiomyocytes. [Abstract]

2024 Jan 24;25(3):1427. PMID: 38338708
Int J Mol Sci

Wnt-Signaling Inhibitor Wnt-C59 Suppresses the Cytokine Upregulation in Multiple Organs of Lipopolysaccharide-Induced Endotoxemic Mice via Reducing the Interaction between β-Catenin and NF-κB. [Abstract]

2021 Jun 10;22(12):6249. PMID: 34200709
iScience

Cardiac fibrosis inhibitor CTPR390 prevents structural and morphological changes in human engineered cardiac connective tissue. [Abstract]

2025 Jun 26;28(8):113013. PMID: 40686602
Oncol Res

Calcyclin-binding protein contributes to cholangiocarcinoma progression by inhibiting ubiquitination of MCM2. [Abstract]

2023 May 24;31(3):317-331. PMID: 37305391
J Med Genet

Association between SCN5A R225Q variant and dilated cardiomyopathy: potential role of intracellular pH and WNT/β-catenin pathway. [Abstract]

2022 Dec;59(12):1139-1149. PMID: 35701104
Stem Cells

Iron Homeostasis Determines Fate of Human Pluripotent Stem Cells Via Glycerophospholipids-Epigenetic Circuit. [Abstract]

2019 Apr;37(4):489-503. PMID: 30599084
Discov Oncol

Propofol inhibits colon cancer cell stemness and epithelial-mesenchymal transition by regulating SIRT1, Wnt/β-catenin and PI3K/AKT/mTOR signaling pathways. [Abstract]

2023 Jul 25;14(1):137. PMID: 37490168
Theriogenology

3, 3', 5-Triiodo-L-thyronine affects polarity proteins of bovine Sertoli cells via WT1/non-canonical Wnt signaling pathway. [Abstract]

2020 May;148:8-17. PMID: 32126395
Theriogenology

Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway. [Abstract]

2019 Oct 15:138:84-93. PMID: 31302435
Exp Ther Med

MicroRNA-139-5p elevates skeletal myogenic differentiation of human adult dental pulp stem cells through Wnt/β-catenin signaling pathway. [Abstract]

2018 Oct;16(4):2835-2842. PMID: 30233663
Korean J Physiol Pharmacol

Wnt-C59 inhibits proinflammatory cytokine expression by reducing the interaction between β-catenin and NF-κB in LPS-stimulated epithelial and macrophage cells. [Abstract]

2021 Jul 1;25(4):307-319. PMID: 34193644
Cell Physiol Biochem

VEGF-A/Neuropilin 1 Pathway Confers Cancer Stemness via Activating Wnt/β-Catenin Axis in Breast Cancer Cells. [Abstract]

2017;44(3):1251-1262. PMID: 29179185

Wnt-C59 purchased from MedChemExpress. Usage Cited in: Cell Physiol Biochem. 2017;44(3):1251-1262. [Abstract]

The protein levels of Wnt3a and β-catenin are examined in MCF-7 spheres and MDA-MB-231 spheres with Wnt-C59 treatment or not via western blot analysis.
bioRxiv

Elongin B orchestrates chromatin and transcriptional programs in H3K27M-mutant diffuse midline glioma. [Abstract]

2026 May 19:2026.05.16.724973. PMID: 42239282
Northwestern University

2026.
Res Sq

2025 Nov 10.
bioRxiv

2025 January 20.
bioRxiv

2024 November 15.
medRxiv

2023 Dec 20.
Patent

US20220370428A1.
Research Square Print

September 14th, 2022.
ACS Comb Sci

Benzimidazolyl-pyrazolo[3,4- b]pyridinones, Selective Inhibitors of MOLT-4 Leukemia Cell Growth and Sea Urchin Embryo Spiculogenesis: Target Quest. [Abstract]

2019 Dec 9;21(12):805-816. PMID: 31689077

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (131.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6354 mL

13.1770 mL

26.3539 mL

5 mM

0.5271 mL

2.6354 mL

5.2708 mL

10 mM

0.2635 mL

1.3177 mL

2.6354 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protokoll

Cell Assay^[2]

1×10⁴ HK1, CNE1, HNE1 and SUNE1 cells are seeded in 24-well plates, and Wnt-C59 (5 μM, 10 μM, and 20 μM) is added the next day. Cell confluence is determined by microscopy at 24, 48, 72, and 96 hours after seeding of cells. The IC₅₀ is determined by MTT assay^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[1]

Mice^[1]

Female nude mice orthotopically transplanted with independent MMTV-WNT1 tumors are treated with vehicle or Wnt-C59 10 mg/kg once daily for 17 days. Tumor volumes are measured on alternate days^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Proffitt KD, et al. Pharmacological inhibition of the Wnt acyltransferase PORCN prevents growth of WNT-driven mammary cancer. Cancer Res. 2013 Jan 15;73(2):502-7. [Content Brief]

[2]. Cheng Y, et al. Wnt-C59 arrests stemness and suppresses growth of nasopharyngeal carcinoma in mice by inhibiting the Wnt pathway in the tumor microenvironment. Oncotarget. 2015 Jun 10;6(16):14428-39. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6354 mL	13.1770 mL	26.3539 mL	65.8848 mL
	5 mM	0.5271 mL	2.6354 mL	5.2708 mL	13.1770 mL
	10 mM	0.2635 mL	1.3177 mL	2.6354 mL	6.5885 mL
	15 mM	0.1757 mL	0.8785 mL	1.7569 mL	4.3923 mL
	20 mM	0.1318 mL	0.6588 mL	1.3177 mL	3.2942 mL
	25 mM	0.1054 mL	0.5271 mL	1.0542 mL	2.6354 mL
	30 mM	0.0878 mL	0.4392 mL	0.8785 mL	2.1962 mL
	40 mM	0.0659 mL	0.3294 mL	0.6588 mL	1.6471 mL
	50 mM	0.0527 mL	0.2635 mL	0.5271 mL	1.3177 mL
	60 mM	0.0439 mL	0.2196 mL	0.4392 mL	1.0981 mL
	80 mM	0.0329 mL	0.1647 mL	0.3294 mL	0.8236 mL
	100 mM	0.0264 mL	0.1318 mL	0.2635 mL	0.6588 mL

Keywords

Wnt-C59

37 Cited Publications

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Wnt-C59

Immunology/Inflammation Compound Library

Stem Cell Signaling Compound Library

Wnt/Hedgehog/Notch Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Anti-Cardiovascular Disease Compound Library

Cytoskeleton Compound Library

Neuroprotective Compound Library

Anti-Breast Cancer Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Human Metabolite Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Antihypertensive Compound Library

Extracellular Vesicles (EVs) Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

Anti-Aging Compound Library Mini

MCE Bioactive Compound Library

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Anti-Gastric Cancer Compound Library

Anti-Fibrosis Compound Library

High-Throughput Bioactive Compound Library

Anti-Rheumatic Arthritis Compound Library

Mass Spectrometry Human Metabolite Library

Publications Citing Use of MedChemExpress (MCE) Wnt-C59

Alle Wnt Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (37)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Wnt-C59 Related Classifications

HY-15659 Related Classifications