Art. -Nr.: HY-B0726A

Pilocarpine

Name: Pilocarpine
Brand: MedChemExpress
SKU: HY-B0726A
Price: 40.00 USD
Availability: InStock
Rating: 5 (46 reviews)

46 Cited Publications

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Based on 46 publication(s) in Google Scholar

Pilocarpine is an orally active, selective M3 muscarinic acetylcholine receptor agonist. Pilocarpine can induce seizures. Pilocarpine is used in research on conditions such as epilepsy, dry mouth (xerostomia), and elevated intraocular pressure.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit: 99.89%
CAS. Nr.: 92-13-7
Formel: C₁₁H₁₆N₂O₂
Molecular Weight:208.26

Speicherung:

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Pilocarpine Chemical Structure

CAS. Nr.: 92-13-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- Induced Disease Models
Chemical Information
Publications (46)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Protokoll
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

mAChR3

Alle mAChR Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	63 μM Compound: Pilocarpine	Stimulation of cAMP in CHO cells expressing human m2 receptor Stimulation of cAMP in CHO cells expressing human m2 receptor	[PMID: 9873644]
HEK293	EC₅₀	0.0175 μM Compound: Pilocarpine	Agonist activity at human m1 muscarinic acetylcholine receptor expressed in HEK293 cells assessed as calcium mobilization by FLIPR assay Agonist activity at human m1 muscarinic acetylcholine receptor expressed in HEK293 cells assessed as calcium mobilization by FLIPR assay	[PMID: 25765911]

In Vitro

To evaluate the cytotoxicity of Pilocarpine, the morphology and viability of human corneal stromal (HCS) cells are examined by light microscopy and MTT assay, respectively. Morphological observations show that HCS cells exposed to Pilocarpine at a concentration from 0.625 to 20 g/L exhibit dose- and time-dependent proliferation retardation and morphological abnormality such as cellular shrinkage, cytoplasmic vacuolation, detachment from culture matrix, and eventually death, while no obvious difference is observed between those exposed to Pilocarpine below the concentration of 0.625 g/L and controls. Results of MTT assay reveal that the cell viability of HCS cells decrease with time and concentration after exposing to Pilocarpine above the concentration of 0.625 g/L (P<0.01 or 0.05), while that of HCS cells treated with Pilocarpine below the concentration of 0.625 g/L show no significant difference to controls^[2]. The partial muscarinic agonist, Pilocarpine, evokes concentration-dependent relaxation with an EC₅₀ of 2.4 mM in isolated segments of rat tail artery that were constricted with Penylephrine (10 to 200 nM)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Induced Disease Models

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Pilocarpine is a cholinergic muscarinic (M) receptor agonist. When used to induce epilepsy, it primarily acts via the central cholinergic system to increase neuronal excitability; this subsequently recruits systems such as the glutamatergic/NMDA pathways, ultimately resulting in status epilepticus (SE)^[4].

Induction of epilepsy^[4]

Background

Pilocarpine induces widespread neuronal excitation and abnormal discharges within limbic system networks by activating central muscarinic cholinergic receptors, progressively leading to recurrent convulsions and status epilepticus (SE). Neuronal damage caused by acute SE and the subsequent remodeling of neural networks are considered key substrates for the later development of epilepsy. Following SE, animals enter a latent period before exhibiting spontaneous recurrent seizures (SRS), thereby establishing a chronic temporal lobe epilepsy phenotype^[4].

Specific Modeling Methods

Mice: Male NMRI mice, 28-32 g (5-6 weeks old)
Administration method: Pilocarpine (250-400 mg/kg) • Intraperitoneal injection, single dose; methscopolamine bromide (HY-B0344) is administered prior to pilocarpine.

Note

(1) Continuous observation of behavioral changes in the animals following drug administration.
(2) The control group must receive the same solvent used for the drug solution to eliminate any effects of the solvent itself.

Modeling Indicators

Histological analysis: Significant remodeling of hippocampal tissue structure, accompanied by neuronal loss and astrogliosis.
EEG assessment: Persistent abnormal electrical activity and seizure-related EEG discharges during status epilepticus (SE).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 92-13-7
Appearance <34°C Solid,>34°C Liquid
Molecular Weight 208.26
Formel C₁₁H₁₆N₂O₂
Color Colorless to light yellow
SMILES

O=C1OC[C@H](CC2=CN=CN2C)[C@@H]1CC
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (46)

All In Vivo Efficacy StudyHistological Imaging/StainingWBIHCIF

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Journal Impact Factor

Immunity

Microglia-derived PDGFB promotes neuronal potassium currents to suppress basal sympathetic tonicity and limit hypertension. [Abstract]

2022 Aug 9;55(8):1466-1482.e9. PMID: 35863346

Pilocarpine purchased from MedChemExpress. Usage Cited in: Immunity. 2022 Aug 9;55(8):1466-1482.e9. [Abstract]

The effects of tamoxifen treatment and microglial PDGFB ablation on pilocarpine (100 mg/kg, ip, once every 20 minutes)-induced epilepsy were investigated by implanting telemetry probes into PDGFBNull-MG mice and analyzing their heart rate (HR) and heart rate variability (HRV) before and 3 or 5 weeks after tamoxifen treatment.
J Nanobiotechnology

Bioengineered anti-EpCAM-guided exosome nanocarriers delivering Foxp3 agonist butyrate achieve tissue-specific immunomodulation in autoimmune Sjögren's syndrome. [Abstract]

2026 Apr 21;24(1):546. PMID: 42015251
J Nanobiotechnology

Improving epilepsy management by targeting P2 × 7 receptor with ROS/electric responsive nanomicelles. [Abstract]

2025 May 5;23(1):332. PMID: 40325469

Pilocarpine purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 May 5;23(1):332. [Abstract]

Seizure scores across different groups of the Pilocarpine (300 mg/kg; i.p.)-induced SE model.

Pilocarpine purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 May 5;23(1):332. [Abstract]

Survival rate analysis among groups of the Pilocarpine (300 mg/kg; i.p.)-induced SE model.

Pilocarpine purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 May 5;23(1):332. [Abstract]

Immunofluorescence staining of CD86 (pro-inflammatory) and CD206 (anti-inflammatory) expression in microglia within the hippocampus across groups of the Pilocarpine (300 mg/kg; i.p.)-induced SE model.
J Nanobiotechnology

miRNA let-7f-5p-encapsulated labial gland MSC-derived EVs ameliorate experimental Sjögren's syndrome by suppressing Th17 cells via targeting RORC/IL-17A signaling axis. [Abstract]

2025 Mar 20;23(1):228. PMID: 40114173
Adv Sci (Weinh)

Microglial GPR35 Ameliorates Epileptogenesis and Neuroinflammation via PDGFA Domain 2 Signaling. [Abstract]

2026 Jan 28:e19642. PMID: 41604576
Acta Pharmacol Sin

G protein-coupled receptor kinase 2 as a novel therapeutic target for gland fibrosis of Sjögren's syndrome. [Abstract]

2024 Dec;45(12):2611-2624. PMID: 39054339
Food Chem

Screening of bioactive flavour compounds targeting muscarinic-3 acetylcholine receptor from Siraitia grosvenorii and evaluation of their synergistic anti-asthmatic activity. [Abstract]

2022 Nov 30:395:133593. PMID: 35792488
Cell Rep

GPNMB and ATP6V1A interact to mediate microglia phagocytosis of multiple types of pathological particles. [Abstract]

2025 Feb 23;44(3):115343. PMID: 39992792
Cell Rep

The ribosome-associated protein RACK1 represses Kir4.1 translation in astrocytes and influences neuronal activity. [Abstract]

2023 Apr 30;42(5):112456. PMID: 37126448

Pilocarpine purchased from MedChemExpress. Usage Cited in: Cell Rep. 2023 Apr 30;42(5):112456. [Abstract]

Latency before the first seizure of PTZ and Pilocarpine ((300 mg/kg; i.p.)-induced seizure tests in RACK1 fl/fl or RACK1 cKO mice.
Cell Mol Neurobiol

Expression Pattern of ALOXE3 in Mouse Brain Suggests Its Relationship with Seizure Susceptibility. [Abstract]

2022 Apr;42(3):777-790. PMID: 33058074

Pilocarpine purchased from MedChemExpress. Usage Cited in: Cell Mol Neurobiol. 2022 Apr;42(3):777-790. [Abstract]

Pilocarpine (325 mg/kg, ip, single dose)-induced status epilepticus (SE) reduced the level of ALOXE3 protein in the hippocampus of mice, but did not reduce it in the temporal lobe cortex.

Pilocarpine purchased from MedChemExpress. Usage Cited in: Cell Mol Neurobiol. 2022 Apr;42(3):777-790. [Abstract]

Pilocarpine (325 mg/kg, ip, single dose)-induced status epilepticus (SE) increases AA levels in the hippocampus of mice.
Ocul Surf

BMAL1 deficiency provokes dry mouth and eyes by down-regulating ITPR2/3. [Abstract]

2024 Oct:34:430-440. PMID: 39343166
Life Sci

Soluble epoxide hydrolase inhibitor, t-AUCB, improves salivary gland function by ameliorating endothelial injury. [Abstract]

2022 Nov 1;308:120942. PMID: 36096247
Cells

Endothelial Myosin IIA Is Required for the Maintenance of Blood-Brain Barrier Integrity. [Abstract]

2024 Oct 1;13(19):1635. PMID: 39404399

Pilocarpine purchased from MedChemExpress. Usage Cited in: Cells. 2024 Oct 1;13(19):1635. [Abstract]

Mice were intravenously injected with Evans blue or sulfonyl-NHS-biotin tracer, followed by intraperitoneal injection of scopolamine, and finally Pilo (300 mg/kg, ip, single dose) was injected to induce epileptic seizures, with close monitoring. The results indicated increased blood-brain barrier permeability in this Pilo-induced epilepsy model.

Pilocarpine purchased from MedChemExpress. Usage Cited in: Cells. 2024 Oct 1;13(19):1635. [Abstract]

Mice were intravenously injected with Evans blue or sulfonyl-NHS-biotin tracer, followed by intraperitoneal injection of scopolamine, and finally Pilo (300 mg/kg, ip, single dose) was injected to induce epileptic seizures, with close monitoring. The results indicated increased blood-brain barrier permeability in this Pilo-induced epilepsy model.

Pilocarpine purchased from MedChemExpress. Usage Cited in: Cells. 2024 Oct 1;13(19):1635. [Abstract]

Mice were intravenously injected with Evans blue or sulfonyl-NHS-biotin tracer, followed by intraperitoneal injection of scopolamine, and finally Pilo (300 mg/kg, ip, single dose) was injected to induce epileptic seizures, with close monitoring. The results indicated increased blood-brain barrier permeability in this Pilo-induced epilepsy model.
Commun Biol

Perturbation of the circadian rhythmic transcription contributes to radiotherapy-induced salivary gland hypofunction. [Abstract]

2026 Jun 25. PMID: 42350790
Commun Biol

An intranasal cationic liposomal polysaccharide vaccine elicits humoral immune responses against Streptococcus pneumoniae. [Abstract]

2024 Sep 17;7(1):1158. PMID: 39284859
Int J Mol Sci

The Activation of Muscarinic Acetylcholine Receptors Protects against Neuroinflammation in a Mouse Model through Attenuating Microglial Inflammation. [Abstract]

2024 Sep 27;25(19):10432. PMID: 39408758
Int Immunopharmacol

CFL1 restores the migratory capacity of bone marrow mesenchymal stem cells in primary Sjögren's syndrome by regulating CCR1 expression. [Abstract]

2024 Feb 15:128:111485. PMID: 38183912
Int J Mol Sci

Immobilization of M3 Muscarinic Receptor to Rapidly Analyze Drug-Protein Interactions and Bioactive Components in a Natural Plant. [Abstract]

2023 Apr 12;24(8):7171. PMID: 37108332
Int J Mol Sci

Structural Insights into M1 Muscarinic Acetylcholine Receptor Signaling Bias between Gαq and β-Arrestin through BRET Assays and Molecular Docking. [Abstract]

2023 Apr 16;24(8):7356. PMID: 37108518
Int Immunopharmacol

Mesenchymal stem cell transplantation alleviates Sjögren's syndrome symptoms by modulating Tim-3 expression. [Abstract]

2022 Oct:111:109152. PMID: 36007392
Mol Neurobiol

The Role of HIF-1α/BNIP3 Pathway in Hippocampal Neuronal Autophagy in Epilepsy. [Abstract]

2025 Dec 1;63(1):232. PMID: 41324728
Neurochem Int

H2S inhibits LiCl/pilocarpine-induced seizures and promotes neuroprotection by regulating TRPV2 expression via the AC3/cAMP/PKA pathway. [Abstract]

2024 Mar:174:105677. PMID: 38290616
Front Pharmacol

HECT, UBA and WWE domain containing 1 represses cholesterol efflux during CD4+ T cell activation in Sjögren's syndrome. [Abstract]

2023 Jun 26:14:1191692. PMID: 37435494
Sci Rep

Inhibiting astrocyte calcium dynamics terminates status epilepticus via wireless septal stimulation. [Abstract]

2025 Jul 15;15(1):25579. PMID: 40664874
Cell Signal

Multi-omics identifies RNF149 as a key molecular marker mediating neuron-glia interaction in temporal lobe epilepsy. [Abstract]

2026 Sep:145:112579. PMID: 42114791
Cell Signal

Clec7a promotes hippocampal microglial activation in rats with status epilepticus via inducing the TLR4/MyD88/NF-κB signaling pathway. [Abstract]

2026 Aug:144:112553. PMID: 42025892
Neuropharmacology

A novel GLP-1/GIP dual receptor agonist protects from 6-OHDA lesion in a rat model of Parkinson's disease. [Abstract]

2017 May 1:117:238-248. PMID: 28223210
J Neurochem

Beta-Arrestin2-Biased Activation by Pilocarpine Suppresses Microglial Inflammatory Response. [Abstract]

2025 Dec;169(12):e70334. PMID: 41457680
Prog Neurobiol

Negative effects of brain regulatory T cells depletion on epilepsy. [Abstract]

2022 Oct:217:102335. PMID: 35931355

Pilocarpine purchased from MedChemExpress. Usage Cited in: Prog Neurobiol. 2022 Oct:217:102335. [Abstract]

The percentages of Foxp3⁺ Tregs among CD4⁺ T cells significantly increased in the hippocampal parenchyma of Pilocarpine(280-300 mg/kg; i.p.)-induced SE mice compared with those in the control group.

Pilocarpine purchased from MedChemExpress. Usage Cited in: Prog Neurobiol. 2022 Oct:217:102335. [Abstract]

GFAP immunostaining in the CA1, CA3, and DG regions of the hippocampi of vehicle-treated control mice, PC61-treated control mice, vehicle-treated Pilocarpine (280-300 mg/kg; i.p.) mice, and PC61-treated Pilocarpine mice.
FASEB J

Pilocarpine mediated excessive calcium accumulation leads to ciliary muscle cell senescence and apoptosis. [Abstract]

2024 Aug 15;38(15):e23878. PMID: 39120551
ACS Pharmacol Transl Sci

Critical Residues in the Gβ Subunit Mediate Selective Recruitment of the Gαq Heterotrimer to Activated G Protein-Coupled Receptors. [Abstract]

2025 Jun 6;8(7):2048-2060. PMID: 40672669
Mol Immunol

MiR-23b-3p alleviates Sjögren's syndrome by targeting SOX6 and inhibiting the NF-κB signaling. [Abstract]

2024 Jun 19:172:68-75. PMID: 38901181
Epilepsia Open

Deficiency of nNOS in adult-born dentate granule cells causes epilepsy. [Abstract]

2025 Aug 28. PMID: 40874679
Mol Cell Endocrinol

Vagotomy suppresses food intake by increasing GLP-1 secretion via the M3 AChR-AMPKα pathway in mice. [Abstract]

2025 Apr 1:599:112464. PMID: 39848433
Brain Sci

Microglia PKM2 Mediates Neuroinflammation and Neuron Loss in Mice Epilepsy through the Astrocyte C3-Neuron C3R Signaling Pathway. [Abstract]

2023 Feb 3;13(2):262. PMID: 36831807
Neuroscience

2026 Sep 28:612:250-261. PMID: 42498064
Neuroscience

Rosiglitazone Prevents Autophagy by Regulating Nrf2-Antioxidant Response Element in a Rat Model of Lithium-pilocarpine-induced Status Epilepticus. [Abstract]

2021 Feb 10:455:212-222. PMID: 33197503
Brain Res

(+)-Borneol exerts neuroprotective effects via suppressing the NF-κB pathway in the pilocarpine-induced epileptogenesis rat model. [Abstract]

2023 Jul 1:1810:148382. PMID: 37127175
Exp Eye Res

Long-term high fructose intake reprograms the circadian transcriptome and disrupts homeostasis in mouse extra-orbital lacrimal glands. [Abstract]

2024 Sep:246:110008. PMID: 39025460
BMC Immunol

Network pharmacology-based strategy to investigate the mechanisms of artemisinin in treating primary Sjögren's syndrome. [Abstract]

2024 Feb 12;25(1):16. PMID: 38347480
PLoS One

Hippocampal transcriptome profiling reveals status epilepticus-induced early changes in gene expression mainly implicating in neuroinflammation and immune responses linked to microglial dysfunction. [Abstract]

2026 Mar 20;21(3):e0344387. PMID: 41860856
J Mol Neurosci

3'UTRs Regulate Mouse Ntrk2 mRNA Distribution in Cortical Neurons. [Abstract]

2020 Nov;70(11):1858-1870. PMID: 32430868
Epilepsy Res

Post-treatment with the GLP-1 analogue liraglutide alleviate chronic inflammation and mitochondrial stress induced by Status epilepticus. [Abstract]

2018 May:142:45-52. PMID: 29549796
Research Square Preprint

2023 Apr 13.
Research Square Print

December 16th, 2022.
Research Square Preprint

2021 Aug.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (480.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.8017 mL

24.0085 mL

48.0169 mL

5 mM

0.9603 mL

4.8017 mL

9.6034 mL

10 mM

0.4802 mL

2.4008 mL

4.8017 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (12.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (12.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (12.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protokoll

Cell Assay^[2]

Cell viability is determined by MTT assay. Briefly, HCS cells are inoculated into a 96-well culture plate (Nunc) at a density of 1×10⁴ cells/100 µL/well, and are cultured and treated. At a 4h interval, the Pilocarpine (0.625 to 20 g/L)-containing medium is replaced entirely with 100 µL serum-free DMEM/F12 medium containing 1.0 g/L MTT, and the cells are incubated at 37°C in the dark for 4h. After the MTT-containing medium is discarded with caution, 150 µL DMSO is added to dissolve the produced formazan crystals at 37°C in the dark for 15 min, and the absorbance at 490 nm is measured with a Multiskan GO microplate reader^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[1]

Rats^[1]
Male, 10-week-old Wistar rats are assigned to one of two groups, exercise (EX, n=6) and control (CN, n=6). The EX rats are kept for 40 days in cages with a running wheel (SN-451), allowing them to undertake voluntary exercise, while the CN rats are kept in cages with the running wheel locked. On the 40th day, Pilocarpine-induced saliva is measured as follows. Briefly, the rats are anesthetized, preweighed cotton was placed in their mouths sublingually, and Pilocarpine (0.5 mg/kg) is intraperitoneally injected to induce saliva secretion. Each cotton ball is then changed every 10 min for 1 h. The collected cotton balls are weighed again, and the mass of saliva secreted is calculated by subtracting the initial from the final weight.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (299 KB)
SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Matsuzaki K, et al. Daily voluntary exercise enhances pilocarpine-induced saliva secretion and aquaporin 1 expression in rat submandibular glands. FEBS Open Bio. 2017 Dec 7;8(1):85-93. [Content Brief]

[2]. Yuan XL, et al. Cytotoxicity of pilocarpine to human corneal stromal cells and its underlying cytotoxic mechanisms. Int J Ophthalmol. 2016 Apr 18;9(4):505-11. [Content Brief]

[3]. Tonta MA, et al. Pilocarpine-induced relaxation of rat tail artery by a non-cholinergic mechanism and in the absence of an intact endothelium. Br J Pharmacol. 1994 Jun;112(2):525-32. [Content Brief]

[4]. Manuela Mazzuferi, et al. Rapid epileptogenesis in the mouse pilocarpine model: Video-EEG, pharmacokinetic and histopathological characterization. Experimental Neurology.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.8017 mL	24.0085 mL	48.0169 mL	120.0423 mL
	5 mM	0.9603 mL	4.8017 mL	9.6034 mL	24.0085 mL
	10 mM	0.4802 mL	2.4008 mL	4.8017 mL	12.0042 mL
	15 mM	0.3201 mL	1.6006 mL	3.2011 mL	8.0028 mL
	20 mM	0.2401 mL	1.2004 mL	2.4008 mL	6.0021 mL
	25 mM	0.1921 mL	0.9603 mL	1.9207 mL	4.8017 mL
	30 mM	0.1601 mL	0.8003 mL	1.6006 mL	4.0014 mL
	40 mM	0.1200 mL	0.6002 mL	1.2004 mL	3.0011 mL
	50 mM	0.0960 mL	0.4802 mL	0.9603 mL	2.4008 mL
	60 mM	0.0800 mL	0.4001 mL	0.8003 mL	2.0007 mL
	80 mM	0.0600 mL	0.3001 mL	0.6002 mL	1.5005 mL
	100 mM	0.0480 mL	0.2401 mL	0.4802 mL	1.2004 mL

Bioz

Keywords

Pilocarpine

46 Cited Publications

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Pilocarpine

Lithium chloride, 99.3%-T

Kainic acid

Acetylcholine chloride

Iperoxo

Xanomeline

NMDA

Aspirin

Bethanechol chloride

Carbamazepine

Dimethyl sulfoxide, meets analytical specification of Ch.P.

L-Glutamine

Theophylline

Publications Citing Use of MedChemExpress (MCE) Pilocarpine

Alle mAChR Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (46)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Bioz

Pilocarpine

Lithium chloride, 99.3%-T

Kainic acid

Acetylcholine chloride

Iperoxo

Xanomeline

NMDA

Aspirin

Bethanechol chloride

Carbamazepine

Dimethyl sulfoxide, meets analytical specification of Ch.P.

L-Glutamine

Theophylline

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Protokoll

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Complete Stock Solution Preparation Table

Pilocarpine Related Classifications

HY-B0726A Related Classifications

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