Vidutolimod

Vidutolimod (2.5-10 μg/mL; 48 h) potently induces IFNα secretion by normal human peripheral blood mononuclear cells^[2].
Vidutolimod (Anti-Qβ-coated) (10 μg/mL; 24 h) significantly induces the upregulation of interferon response-related genes and immune communication-related genes (including CXCL10, PDL1, IDO, and CD80) in monocytes derived from peripheral blood mononuclear cells (PBMCs) of healthy donors^[3].
Vidutolimod (Anti-Qβ-coated) (10 μg/mL; 24 h) indirectly induces CXCL10 production in monocytes from healthy donors via type I interferons (primarily IFN-β) produced by plasmacytoid dendritic cells (pDCs), and this process does not require intercellular contact between pDCs and monocytes^[3].
Vidutolimod (Anti-Qβ-coated) (10 μg/mL; 24 h) enhances the expression of IDO and PDL1 in monocytes from healthy donors. The induction of IDO depends on the type I IFN signaling pathway mediated by IFNAR2, while the induction of PDL1 may involve other non-IFN-dependent factors^[3].
Vidutolimod (Anti-Qβ-coated) (10 μg/mL; 1 h uptake assay, 18-20 h phenotype analysis) regulates the IFN-α-induced responses of PDL1 and CXCL10 in monocytes from healthy donors in a CD32a-mediated phagocytosis-dependent manner, while the regulation of IDO also involves other Fc receptor signaling pathways (e.g., CD64)^[3].
Treatment with Vidutolimod (Anti-Qβ-coated) (10 μg/mL; 18-20 h) and IFN-α upregulates CD80 expression on monocytes from healthy donors and enhances their capacity to induce the proliferation of autologous CD4⁺ T cells^[3].
Combination of vidutolimod (5 µg/mL; 24 h) with anti-Qβ activates human plasmacytoid dendritic cells (pDCs) and monocytes^[4].
Combined with anti-Qβ, Vidutolimod (5 µg/mL; 24 h) activates primary human CD4⁺ T cells, CD8⁺ T cells and NK cells, and increases the secretion levels of IFNγ and TNFα in a co-culture system with HPV⁺ UM-SCC47 head and neck squamous cell carcinoma cells^[4].
Combination of Vidutolimod and anti-Qβ directly activates isolated primary human NK cells co-cultured with HPV⁺ UM-SCC47 HNSCC cells, yet this response is weaker than that observed in the co-culture system of intact peripheral blood mononuclear cells (PBMC) in the presence of dendritic cells (DC)^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.