BOT-64
Based on 2 publication(s) in Google Scholar
BOT-64 is an inhibitory κB (IκB) kinase β (IKKβ) inhibitor with an IC50 of 1 µM. BOT-64 blocks lipopolysaccharide-induced nuclear factor-κB activation and nuclear factor-κB-regulated inflammatory gene transcription.
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- Pureté: 98.87%
- CAS No.: 113760-29-5
- Formule: C15H15NO2S
- Masse moléculaire:273.35
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) BOT-64
More
Activité biologique
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IKKβ 1 μM (IC50) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| ACHN | GI50 |
8.98 μM
Compound: 1a
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Antiproliferative activity against human ACHN cells by SRB assay
Antiproliferative activity against human ACHN cells by SRB assay
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[PMID: 22738641] |
| HCT-15 | GI50 |
8.09 μM
Compound: 1a
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Antiproliferative activity against human HCT15 cells by SRB assay
Antiproliferative activity against human HCT15 cells by SRB assay
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[PMID: 22738641] |
| MDA-MB-231 | GI50 |
5.88 μM
Compound: 1a
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Antiproliferative activity against human MDA-MB-231 cells by SRB assay
Antiproliferative activity against human MDA-MB-231 cells by SRB assay
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[PMID: 22738641] |
| NCI-H23 | GI50 |
7.08 μM
Compound: 1a
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Antiproliferative activity against human NCI-H23 cells by SRB assay
Antiproliferative activity against human NCI-H23 cells by SRB assay
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[PMID: 22738641] |
| NUGC-3 | GI50 |
5.46 μM
Compound: 1a
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Antiproliferative activity against human NUGC3 cells by SRB assay
Antiproliferative activity against human NUGC3 cells by SRB assay
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[PMID: 22738641] |
| PC-3 | GI50 |
7.4 μM
Compound: 1a
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Antiproliferative activity against human PC3 cells by SRB assay
Antiproliferative activity against human PC3 cells by SRB assay
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[PMID: 22738641] |
| RAW264.7 | IC50 |
6.2 μM
Compound: 1a
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Inhibition of NFkappaB expressed in RAW264.7 cells coexpressing NPT assessed as inhibition of LPS-induced SEAP activity incubated for 2 hrs prior to LPS-induction measured after 16 hrs by reporter gene assay
Inhibition of NFkappaB expressed in RAW264.7 cells coexpressing NPT assessed as inhibition of LPS-induced SEAP activity incubated for 2 hrs prior to LPS-induction measured after 16 hrs by reporter gene assay
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[PMID: 22738641] |
| THP-1 | IC50 |
6.2 μM
Compound: 1a
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Inhibition of NFkappaB expressed in THP1 cells assessed as inhibition of LPS-induced SEAP activity incubated for 2 hrs prior to LPS-induction measured after 16 hrs by reporter gene assay
Inhibition of NFkappaB expressed in THP1 cells assessed as inhibition of LPS-induced SEAP activity incubated for 2 hrs prior to LPS-induction measured after 16 hrs by reporter gene assay
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[PMID: 22738641] |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 113760-29-5
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Appearance Solid
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Masse moléculaire 273.35
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Formule C15H15NO2S
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Color Off-white to light yellow
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SMILES
O=C1CC(C)(C)CC2=C1S/C(O2)=N\C3=CC=CC=C3
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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Nature
2026 Apr;652(8110):740-751. PMID: 41741641 -
Biol Direct
PNSC928, a plant-derived compound, specifically disrupts CtBP2-p300 interaction and reduces inflammation in mice with acute respiratory distress syndrome. [Abstract]2024 Jun 21;19(1):48. PMID: 38902802
Solvant et solubilité
DMSO : 100 mg/mL (365.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.15 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.15 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (273 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.6583 mL | 18.2916 mL | 36.5831 mL | 91.4578 mL |
| 5 mM | 0.7317 mL | 3.6583 mL | 7.3166 mL | 18.2916 mL | |
| 10 mM | 0.3658 mL | 1.8292 mL | 3.6583 mL | 9.1458 mL | |
| 15 mM | 0.2439 mL | 1.2194 mL | 2.4389 mL | 6.0972 mL | |
| 20 mM | 0.1829 mL | 0.9146 mL | 1.8292 mL | 4.5729 mL | |
| 25 mM | 0.1463 mL | 0.7317 mL | 1.4633 mL | 3.6583 mL | |
| 30 mM | 0.1219 mL | 0.6097 mL | 1.2194 mL | 3.0486 mL | |
| 40 mM | 0.0915 mL | 0.4573 mL | 0.9146 mL | 2.2864 mL | |
| 50 mM | 0.0732 mL | 0.3658 mL | 0.7317 mL | 1.8292 mL | |
| 60 mM | 0.0610 mL | 0.3049 mL | 0.6097 mL | 1.5243 mL | |
| 80 mM | 0.0457 mL | 0.2286 mL | 0.4573 mL | 1.1432 mL | |
| 100 mM | 0.0366 mL | 0.1829 mL | 0.3658 mL | 0.9146 mL |
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