Mitraphylline
Based on 1 Customer Validation
Mitraphylline is the major pentacyclic oxindolic alkaloid presented in Uncaria tomentosa. Mitraphylline inhibits lipopolysaccharide-mediated activation of primary human neutrophils. Mitraphylline exhibits antiproliferative activity in human glioma and neuroblastoma cell lines. Mitraphylline can inhibit interleukin release in vivo.
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- Pureté : 99.61%
- CAS No.: 509-80-8
- Formule: C21H24N2O4
- Masse moléculaire:368.43
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Stockage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| GAMG | IC50 |
12.3 μM
Compound: 20
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Anticancer activity against human GAMG cells assessed as cell growth inhibition
Anticancer activity against human GAMG cells assessed as cell growth inhibition
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[PMID: 27543880] |
| SK-N-BE(2) | IC50 |
20 μM
Compound: 20
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Anticancer activity against human SKNBE2 cells assessed as cell growth inhibition
Anticancer activity against human SKNBE2 cells assessed as cell growth inhibition
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[PMID: 27543880] |
In Vitro
Mitraphylline (25 μM) significantly reduces the NO production in human LPS-activated neutrophils[1].
Mitraphylline (25 μM, 6 h) significantly downregulates the pro-inflammatory cytokines in LPS-treated neutrophils[1].
Mitraphylline (0-40 μM, 48 h) inhibits cell viability dose-dependently in both GAMG (IC50 = 20.05 μM) and SKN-BE cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:LPS-treated neutrophils
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Concentration:25 μM
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Incubation Time:6 h
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Result:Triggered neutrophils changes towards anti-inflammatory phenotype.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Eight-week-old Balb/c female mice[2]
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Dosage:30 mg/kg/day
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Administration:Oral gavage (p.o.), 3 d
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Result:Inhibited nearly 70% of the release of IL-1α and IL-1β.
Impaired the liberation of TNF-α by 50%.
Chemical Information
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CAS No. 509-80-8
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Appearance Solid
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Masse moléculaire 368.43
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Formule C21H24N2O4
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Color White to off-white
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SMILES
O=C1NC2=C([C@@]13CCN4C[C@@]5([C@@H](OC=C([C@]5(C[C@]43[H])[H])C(OC)=O)C)[H])C=CC=C2
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvant et solubilité
In Vitro:
DMSO : 6.76 mg/mL (18.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocole
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureté et documentation
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Fiche technique (285 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Montserrat-de la Paz S, et al. Mitraphylline inhibits lipopolysaccharide-mediated activation of primary human neutrophils. Phytomedicine. 2016 Feb 15;23(2):141-8. [Content Brief]
[2]. Rojas-Duran, R., et al., (2012). Anti-inflammatory activity of Mitraphylline isolated from Uncaria tomentosa bark. Journal of ethnopharmacology, 143(3), 801–804. [Content Brief]
[3]. García Prado, E., et al., (2007). Antiproliferative effects of mitraphylline, a pentacyclic oxindole alkaloid of Uncaria tomentosa on human glioma and neuroblastoma cell lines. Phytomedicine : international journal of phytotherapy and phytopharmacology, 14(4), 280–284. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7142 mL | 13.5711 mL | 27.1422 mL | 67.8555 mL |
| 5 mM | 0.5428 mL | 2.7142 mL | 5.4284 mL | 13.5711 mL | |
| 10 mM | 0.2714 mL | 1.3571 mL | 2.7142 mL | 6.7855 mL | |
| 15 mM | 0.1809 mL | 0.9047 mL | 1.8095 mL | 4.5237 mL |
Mitraphylline Related Classifications
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