N-Nitrosomorpholine
Based on 1 Customer Validation
N-Nitrosomorpholine is a photosensitive nitrosamine that is carcinogenic and can be used to construct animal cancer models.
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- Pureté: 99.98%
- CAS No.: 59-89-2
- Formule: C4H8N2O2
- Masse moléculaire:116.12
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Stockage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.
Administration: 160 mg/L • mixed in drinking water • daily for 80 weeks
Administration: 200 mg/L• mixed in drinking water• daily for 80 weeks
(2) After killing the rats with a rapid spinal dislocation, Livers were quickly extirpated and pieces of about 1 cm x 0.5 cm from the right and left liver lobe were frozen in liquid nitrogen-cooled isopentane and stored at -80 °C.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 59-89-2
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Appearance <29°C Solid,>29°C Liquid
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Masse moléculaire 116.12
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Formule C4H8N2O2
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Color Light yellow to yellow
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SMILES
O=NN1CCOCC1
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
DMSO : 100 mg/mL (861.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (21.53 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (21.53 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (278 KB)
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SDS (644 KB)
- English - EN (644 KB)
- Français - FR (644 KB)
- Deutsch - DE (644 KB)
- Norwegian - NO (644 KB)
- Español - ES (644 KB)
- Swedish - SV (644 KB)
- Italian - IT (644 KB)
- Korean - KR (644 KB)
- Portuguese - PT (644 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Kobusch AB, et al. Tumor-promoting activity and cytotoxicity of 3,4,3',4'-tetrachlorobiphenyl on N-nitrosomorpholine-induced murine liver foci. J Cancer Res Clin Oncol. 1989;115(3):247-52. [Content Brief]
[2]. Enzmann H, et al. Elevation of glucose-6-phosphate in early stages of hepatocarcinogenesis induced in rats by N-nitrosomorpholine. Horm Metab Res. 1988 Feb;20(2):128-9. [Content Brief]
[3]. Weber E, et al. Dose and time dependence of the cellular phenotype in rat hepatic preneoplasia and neoplasia induced by continuous oral exposure to N-nitrosomorpholine. Carcinogenesis. 1994 Jun;15(6):1235-42. [Content Brief]
[4]. K D Brunnemann, et al. N-Nitrosomorpholine and Other Volatile N-nitrosamines in Snuff Tobacco. Carcinogenesis. 1982;3(6):693-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 8.6118 mL | 43.0589 mL | 86.1178 mL | 215.2945 mL |
| 5 mM | 1.7224 mL | 8.6118 mL | 17.2236 mL | 43.0589 mL | |
| 10 mM | 0.8612 mL | 4.3059 mL | 8.6118 mL | 21.5295 mL | |
| 15 mM | 0.5741 mL | 2.8706 mL | 5.7412 mL | 14.3530 mL | |
| 20 mM | 0.4306 mL | 2.1529 mL | 4.3059 mL | 10.7647 mL | |
| 25 mM | 0.3445 mL | 1.7224 mL | 3.4447 mL | 8.6118 mL | |
| 30 mM | 0.2871 mL | 1.4353 mL | 2.8706 mL | 7.1765 mL | |
| 40 mM | 0.2153 mL | 1.0765 mL | 2.1529 mL | 5.3824 mL | |
| 50 mM | 0.1722 mL | 0.8612 mL | 1.7224 mL | 4.3059 mL | |
| 60 mM | 0.1435 mL | 0.7176 mL | 1.4353 mL | 3.5882 mL | |
| 80 mM | 0.1076 mL | 0.5382 mL | 1.0765 mL | 2.6912 mL | |
| 100 mM | 0.0861 mL | 0.4306 mL | 0.8612 mL | 2.1529 mL |
N-Nitrosomorpholine Related Classifications
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