Orcinol
Based on 1 Customer Validation
Orcinol (3,5-Dihydroxytoluene) is an organic compound used in biological dyeing and proteomics research. Orcinol inhibits melanogenesis in B16F10 cells by upregulating the MAPK/ERK signaling pathway, and suppresses the expression of MITF, tyrosinase (TYR), TRP1, and DCT. Orcinol exhibits certain DPPH radical scavenging activity. In addition, Orcinol can alter nitrogen balance in animals. Orcinol holds promise for research in cancer and metabolic diseases.
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- Pureté: 99.97%
- CAS No.: 504-15-4
- Formule: C7H8O2
- Masse moléculaire:124.14
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Stockage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
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COX-2 |
Orcinol (0.08-3 mM, 24 hours) exhibites low radical scavenging activity and cytotoxicity[1]. Orcinol (0.5-1 mM, 72 hours treatment) inhibites melanogenesis in B16F10 cells[2]. Orcinol (0.5-1 mM, 24 hours) inhibites the expression of melanogenesis-related genes by upregulating the MAPK/ERK signaling pathway and inhibiting the expression of melanogenesis-related genes such as MITF[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RAW264.7 mouse macrophage-like cells
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Concentration:0.08-3 mM
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Incubation Time:24 hours
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Result:Exhibited weak radical scavenging activity and relatively low cytotoxicity, with an LC50 of 3 mM and weak DPPH radical scavenging activity.
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Cell Line:B16F10 mouse melanoma cells
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Concentration:0.5-1 mM
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Incubation Time:72 hours
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Result:Significantly reduced intracellular tyrosinase activity and melanin content.
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Cell Line:B16F10 mouse melanoma cells
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Concentration:0.5-1 mM
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Incubation Time:24 hours
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Result:Significantly downregulated the mRNA levels of MITF, TYR, TRP1, and DCT.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Common brushtail possum (Trichosurus vulpecula)[3]
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Dosage:2%, included in diet
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Administration:Dietary intake (2% Orcinol included in diet), 9 days
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Result:Caused an immediate reduction in food intake, which returned to normal after 2 days. Urine pH dropped from 7.8 to 5.1, with increased excretion of ammonium nitrogen. No signs of protein catabolism were observed.
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Chemical Information
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CAS No. 504-15-4
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Appearance Solid
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Masse moléculaire 124.14
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Formule C7H8O2
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Color White to yellow
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SMILES
OC1=CC(C)=CC(O)=C1
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Synonyms
3,5-Dihydroxytoluene
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvant et solubilité
DMSO : 100 mg/mL (805.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Pureté et documentation
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Fiche technique (279 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Murakami Y, et al. The Radical Scavenging Activity and Cytotoxicity of Resveratrol, Orcinol and 4-Allylphenol and their Inhibitory Effects on Cox-2 Gene Expression and Nf-κb Activation in RAW264.7 Cells Stimulated with Porphyromonas gingivalis-fimbriae. In Vivo. 2015 May-Jun;29(3):341-9. [Content Brief]
[3]. Edwards MJ, et al. Acid loads induced by the detoxification of plant secondary metabolites do not limit feeding by common brushtail possums (Trichosurus vulpecula). J Comp Physiol B. 2010 Feb;180(2):247-57. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 8.0555 mL | 40.2774 mL | 80.5549 mL | 201.3872 mL |
| 5 mM | 1.6111 mL | 8.0555 mL | 16.1110 mL | 40.2774 mL | |
| 10 mM | 0.8055 mL | 4.0277 mL | 8.0555 mL | 20.1387 mL | |
| 15 mM | 0.5370 mL | 2.6852 mL | 5.3703 mL | 13.4258 mL | |
| 20 mM | 0.4028 mL | 2.0139 mL | 4.0277 mL | 10.0694 mL | |
| 25 mM | 0.3222 mL | 1.6111 mL | 3.2222 mL | 8.0555 mL | |
| 30 mM | 0.2685 mL | 1.3426 mL | 2.6852 mL | 6.7129 mL | |
| 40 mM | 0.2014 mL | 1.0069 mL | 2.0139 mL | 5.0347 mL | |
| 50 mM | 0.1611 mL | 0.8055 mL | 1.6111 mL | 4.0277 mL | |
| 60 mM | 0.1343 mL | 0.6713 mL | 1.3426 mL | 3.3565 mL | |
| 80 mM | 0.1007 mL | 0.5035 mL | 1.0069 mL | 2.5173 mL | |
| 100 mM | 0.0806 mL | 0.4028 mL | 0.8055 mL | 2.0139 mL |
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