OSU-2S TFA
Based on 1 publication(s) in Google Scholar
OSU-2S TFA is a non-immunosuppressive derivative of Fingolimod hydrochloride (FTY720) (HY-12005). It is an orally active, blood-brain barrier-permeable allosteric activator of PP2A that also activates PKCδ; it restores PP2A activity by displacing the inhibitory protein SET, thereby inducing ROS-PKCδ-caspase-3-dependent apoptosis, c-Myc degradation, cell cycle arrest, and differentiation. OSU-2S TFA is used in research on diseases such as leukemia, liver cancer, lymphoma, and lung cancer.
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- Formule: C23H38F3NO4
- Masse moléculaire:449.55
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Stockage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) OSU-2S TFA
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Activité biologique
Description
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PKCδ |
In Vitro
OSU-2S (2-6 μM; 24 h) TFA exhibits concentration-dependent cytotoxicity against canine B-cell lymphoma cell lines (CLBL-1 and 17–71)[7].
The cytotoxicity of OSU-2S (3 μM; 24 h) TFA in canine B-cell lymphoma cell lines is partially mediated by ROS[7].
OSU-2S (2-4 μM; 24 h) TFA induces ROS production and mediates cytotoxicity in primary cells from canine spontaneous B-cell lymphoma[7].
OSU-2S (24 h) TFA downregulates PGC1α and inhibits mitochondrial biogenesis via PP2A-dependent FOXO1 dephosphorylation[6].
OSU-2S TFA reduces mitochondrial mass and ATP production, and inhibits the expression of genes associated with mitochondrial biogenesis[6].
OSU-2S (2 μM; 24 h) TFA induces apoptosis in primary CLL cells[4].
OSU-2S TFA activates PP2A in CLL cells[4].
OSU-2S (5 h) TFA induces SHP1 S591 phosphorylation via a PKC-dependent mechanism[4].
OSU-2S (5-24 h) TFA -induced pSHP1 S591 levels correlate with CLL cell death[4].
The cytotoxicity of OSU-2S (2 μM; 24 h) TFA is partially dependent on PKC[4].
OSU-2S (8 μM; 4 h) TFA activates PKC in CLL cells[4].
OSU-2S (8 μM; 16 h) TFA extensively regulates gene expression in CLL cells, affecting processes such as cell growth, proliferation, death, and the cell cycle[4].
OSU-2S (8 μM; 16-24 h) TFA downregulates TCL1A expression in CLL cells[4].
OSU-2S (5 μM; 24 h) TFA selectively kills CLL cells without affecting normal B cells via ROR1-targeted delivery[4].
OSU-2S (2-8 μM; 24 h) TFA exhibits potent cytotoxicity against MCL cell lines[3].
OSU-2S (2-8 μM; 24 h) TFA induces apoptosis in MCL cell lines[3].
OSU-2S (4-5 μM; 24 h) TFA induces CD74 expression on the surface of MCL cells[3].
OSU-2S (4 μM; 24 h) TFA enhances the cytotoxicity of Milatuzumab (HY-P99731) against MCL cells by upregulating CD74[3].
OSU-2S (2-10 μM; 48 h) TFA demonstrates anti-proliferative activity against various HCC cell lines[2].
OSU-2S (1.25-2.5 μM; 48 h) TFA enhances the anti-proliferative effect of Sorafenib (HY-10201) on HCC cells, exhibiting a synergistic effect[2].
OSU-2S (1.25-2.5 μM; 48 h) TFA enhances Sorafenib-induced apoptosis in HCC cells[2].
OSU-2S (1.25-2.5 μM; 48 h) TFA enhances the anti-HCC effect of Sorafenib via PKCδ and ERK pathways[2].
OSU-2S (1.25-2.5 μM; 8-24 h) TFA in combination with Sorafenib inhibits HCC cell migration and invasion[2].
The activity of OSU-2S (1.25-7.5 μM) TFA against HCC cells is influenced by p53 status; p53 restoration enhances sensitivity[2].
OSU-2S (1-10 μM; 24 h) TFA inhibits A549 proliferation, with an IC50 of 4.432 μM[1].
OSU-2S (1.5-6 μM; 14 days) TFA inhibits colony formation in A549 cells[1].
OSU-2S (1.5-6 μM; 12-24 h) TFA inhibits A549 cell migration[1].
OSU-2S (1.5-6 μM; 24 h) TFA downregulates p-AURKA and S1PR1 protein expression in A549 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Canine B-cell lymphoma cell lines CLBL-1 and 17-71
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Concentration:2 μM; 4 μM; 6 μM
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Incubation Time:24 h
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Result:Promoted cytotoxicity at concentrations as low as 2 μM.
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Cell Line:Primary cells from canine spontaneous B-cell lymphoma
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Concentration:2 μM; 4 μM; 8 μM
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Incubation Time:24 h
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Result:Induced apoptosis in canine lymphoma cells.
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Cell Line:Primary CD19+ cells from CLL patients
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Concentration:2 μM
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Incubation Time:24 h
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Result:Induced apoptosis in primary CLL cells.
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Cell Line:CLL cells
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Concentration:8 μM
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Incubation Time:16 h
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Result:Reduced TCL1A mRNA levels.
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Cell Line:CLL cells
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Concentration:8 μM
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Incubation Time:24 h
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Result:Reduced TCL1A protein levels.
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Cell Line:MCL cell line
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Concentration:5 μM
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Incubation Time:24 h
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Result:Induced apoptosis in MCL cell lines.
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Cell Line:Huh7, Hep3B, PLC5, HepG2
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Concentration:2 μM; 4 μM; 6 μM; 8 μM; 10 μM
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Incubation Time:48 h
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Result:Inhibited cell proliferation, with HepG2 being the most sensitive.
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Cell Line:A549
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Concentration:1.5 μM; 3 μM; 6 μM
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Incubation Time:12 h; 24 h
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Result:Reduced the migration ability of A549 cells.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Subcutaneous inoculation of A549 cells into BALB/c nude mice[1]
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Dosage:2.5 mg/kg, 5 mg/kg, 10 mg/kg
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Administration:i.p.; every other day for 35 days
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Result:Tumor volume and weight were significantly reduced.
There was no significant decrease in mouse body weight.
Immunohistochemistry showed that OSU-2S downregulated Ki-67 and VEGF.
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Flow cytometric DNA-content cell-cycle staining
- Annexin V plus membrane-impermeant dye apoptosis staining
- ROS/oxidative-stress fluorescent staining
- BrdU Incorporation Assay
- Apoptosis Solutions
- Protocol for Cell Cycle
- Liver Cancer Modeling
Chemical Information
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Appearance Solid
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Masse moléculaire 449.55
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Formule C23H38F3NO4
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SMILES
CCC[C@@](CCC1=CC=C(OCCCCCC(C)C)C=C1)(N)CO.O=C(O)C(F)(F)F
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Int Immunopharmacol
Osthole alleviates neuropathic pain by suppressing astrocytes activation and associated inflammatory responses via the PKCδ/TRPV4 signaling pathway. [Abstract]2025 Sep 10:165:115453. PMID: 40934539
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (222.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2244 mL | 11.1222 mL | 22.2445 mL | 55.6112 mL |
| 5 mM | 0.4449 mL | 2.2244 mL | 4.4489 mL | 11.1222 mL | |
| 10 mM | 0.2224 mL | 1.1122 mL | 2.2244 mL | 5.5611 mL | |
| 15 mM | 0.1483 mL | 0.7415 mL | 1.4830 mL | 3.7074 mL | |
| 20 mM | 0.1112 mL | 0.5561 mL | 1.1122 mL | 2.7806 mL | |
| 25 mM | 0.0890 mL | 0.4449 mL | 0.8898 mL | 2.2244 mL | |
| 30 mM | 0.0741 mL | 0.3707 mL | 0.7415 mL | 1.8537 mL | |
| 40 mM | 0.0556 mL | 0.2781 mL | 0.5561 mL | 1.3903 mL | |
| 50 mM | 0.0445 mL | 0.2224 mL | 0.4449 mL | 1.1122 mL | |
| 60 mM | 0.0371 mL | 0.1854 mL | 0.3707 mL | 0.9269 mL | |
| 80 mM | 0.0278 mL | 0.1390 mL | 0.2781 mL | 0.6951 mL | |
| 100 mM | 0.0222 mL | 0.1112 mL | 0.2224 mL | 0.5561 mL |
OSU-2S TFA Related Classifications
HY-117720A Related Classifications
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