Cat. No.: HY-N3595

Cleomiscosin A

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Based on 1 Customer Validation

Cleomiscosin A is a coumarino-lignoid from branch of Macaranga adenantha. Cleomiscosin A is active against TNF-alpha secretion of the mouse peritoneal macrophages.

For research use only. We do not sell to patients.

Purity: 99.15%
CAS No.: 76948-72-6
Formula: C₂₀H₁₈O₈
Molecular Weight:386.35

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Cleomiscosin A Chemical Structure

CAS No.: 76948-72-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cleomiscosin A is a coumarino-lignoid from branch of Macaranga adenantha. Cleomiscosin A is active against TNF-alpha secretion of the mouse peritoneal macrophages^[1]^[2].

All TNF Receptor Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
BV-2	IC₅₀	>100 μM Compound: 2	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay	[PMID: 27919656]
KB	ED₅₀	>10 μg/mL Compound: cleomiscosin-A	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 6481366]
KB	ED₅₀	4.9 μg/mL Compound: 1, NSC-346197	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 6736970]
KB	ED₅₀	4.9 μg/mL Compound: 4	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 6619884]
P388	ED₅₀	0.4 μg/mL Compound: cleomiscosin-A	Cytotoxicity against mouse P388 cells Cytotoxicity against mouse P388 cells	[PMID: 6481366]
P388	ED₅₀	2.8 μg/mL Compound: 4	Cytotoxicity against mouse P388 cells Cytotoxicity against mouse P388 cells	[PMID: 6619884]
P388	ED₅₀	3.8 μg/mL Compound: 1, NSC-346197	Cytotoxicity against mouse P388 cells Cytotoxicity against mouse P388 cells	[PMID: 6736970]

Chemical Information

CAS No. 76948-72-6
Appearance Solid
Molecular Weight 386.35
Formula C₂₀H₁₈O₈
Color White to off-white
SMILES

COC1=CC(C=CC2=O)=C(O2)C3=C1O[C@H](C4=CC(OC)=C(O)C=C4)[C@@H](CO)O3
Structure Classification
- Phenylpropanoids
- Lignans
- Phenols
- Monophenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (323.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5883 mL

12.9416 mL

25.8833 mL

5 mM

0.5177 mL

2.5883 mL

5.1767 mL

10 mM

0.2588 mL

1.2942 mL

2.5883 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (5.38 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Sharma S, et al. Synthesis and anti-inflammatory activity of derivatives of coumarino-lignoid, cleomiscosin A and its methyl ether. Eur J Med Chem. 2010;45(11):5150-5156. [Content Brief]

[2]. Ma M, et al. Zhongguo Zhong Yao Za Zhi. 2007;32(12):1175-1179. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.5883 mL	12.9416 mL	25.8833 mL	64.7082 mL
	5 mM	0.5177 mL	2.5883 mL	5.1767 mL	12.9416 mL
	10 mM	0.2588 mL	1.2942 mL	2.5883 mL	6.4708 mL
	15 mM	0.1726 mL	0.8628 mL	1.7256 mL	4.3139 mL
	20 mM	0.1294 mL	0.6471 mL	1.2942 mL	3.2354 mL
	25 mM	0.1035 mL	0.5177 mL	1.0353 mL	2.5883 mL
	30 mM	0.0863 mL	0.4314 mL	0.8628 mL	2.1569 mL
	40 mM	0.0647 mL	0.3235 mL	0.6471 mL	1.6177 mL
	50 mM	0.0518 mL	0.2588 mL	0.5177 mL	1.2942 mL
	60 mM	0.0431 mL	0.2157 mL	0.4314 mL	1.0785 mL
	80 mM	0.0324 mL	0.1618 mL	0.3235 mL	0.8089 mL
	100 mM	0.0259 mL	0.1294 mL	0.2588 mL	0.6471 mL

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Cleomiscosin A

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All TNF Receptor Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Cleomiscosin A Related Classifications

HY-N3595 Related Classifications