ddhCTP 

ddhCTP is an endogenously produced pyrimidine base analog with a K_d of 17.0 nM for LLDH and an IC₅₀ of 55.8 μM for GAPDH. By inhibiting key metabolic enzymes such as GAPDH, ddhCTP reduces glycolytic flux and shifts metabolic flow toward the pentose phosphate pathway, thereby regulating the redox balance of cells. As a competitive CTP analog, ddhCTP terminates RNA synthesis by flavivirus RdRps and SARS-CoV-2 RdRp, and inhibits Zika virus replication in vivo. ddhCTP can be used in studies related to viral infections, COVID-19 and Zika virus infections.^[1][2]

ddhCTP (24-96 h) completely abolishes the extracellular levels of phenylacetic acid and 4-hydroxyphenyllactic acid in HEK293 cells expressing wild-type RSAD2^[1].
ddhCTP (72 h) modulates the levels of NAD⁺-dependent enzyme-related metabolites, including decreased taurine levels, increased 2-oxoglutarate levels, increased glyceraldehyde 3-phosphate levels, and increased levels of pentose phosphate pathway intermediates in hiPSC-derived macrophages expressing wild-type RSAD2 after stimulation with LPS and IFN-γ^[1].
ddhCTP inhibits the NAD⁺-dependent forward activity of purified rabbit muscle GAPDH with an IC₅₀ of 55.8 μM, but does not affect the NADH-dependent reverse reaction^[1].
ddhCTP (72 μM, 30 s) inhibits the NAD⁺-dependent activities of purified rabbit muscle LLDH and purified bovine heart MDH, but does not affect the NADH-dependent reverse reaction of LLDH^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

465.14

C₉H₁₄N₃O₁₃P₃

2279171-34-3

NC(C=CN1[C@@H]2OC(COP(OP(OP(O)(O)=O)(O)=O)(O)=O)=C[C@H]2O)=NC1=O

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Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Honarmand Ebrahimi K, et al. ddhCTP produced by the radical-SAM activity of RSAD2 (viperin) inhibits the NAD⁺ -dependent activity of enzymes to modulate metabolism. FEBS Lett. 2020;594(10):1631-1644.

  [2]. Ciardullo G, et al. Viral RNA Replication Suppression of SARS-CoV-2: Atomistic Insights into Inhibition Mechanisms of RdRp Machinery by ddhCTP. J Chem Inf Model. 2024;64(5):1593-1604.  [Content Brief]