Dephospho-CoA
Based on 1 publication(s) in Google Scholar
Dephospho-CoA is a key intermediate in the biosynthesis of coenzyme A (CoA), which is catalyzed to form CoA by GTP-dependent Dephospho-CoA kinase (DPCK). Dephospho-CoA completes the final synthetic step of CoA via phosphorylation and participates in energy metabolism and cellular signal transduction. Dephospho-CoA can be used in studies involving cancer (e.g., regulation of cell proliferation) and metabolic diseases (e.g., mitochondrial dysfunction).
For research use only. We do not sell to patients.
- Purity: 96%
- CAS No.: 3633-59-8
- Formula: C21H35N7O13P2S
- Molecular Weight:687.55
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Dephospho-CoA
MoreAll Endogenous Metabolite Isoforms
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Biological Activity
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Human Endogenous Metabolite |
Dephospho-CoA (10 min) is quantitatively phosphorylated to CoA by DPCK, as confirmed by reverse-phase HPLC analysis of reaction products[1].
Dephospho-CoA (1 mM; 80 °C incubation for standard/kinetic assays; 70 °C incubation for specificity assays; 2 h preincubation for thermostability assays) was specifically phosphorylated to CoA by purified recombinant Thermococcus kodakarensis TK1697 protein, a GTP-dependent DPCK with optimal activity at 80 °C and pH 8.0, high thermostability, and a catalytic efficiency (kcat/Km) of 40.4 s−1 mM−1 for dephospho-CoA[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 3633-59-8
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Appearance Solid
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Molecular Weight 687.55
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Formula C21H35N7O13P2S
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Color White to off-white
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SMILES
O[C@H]([C@@H]1O)[C@](O[C@@H]1CO[P](O[P](OCC(C)(C)[C@@H](O)C(NCCC(NCCS)=O)=O)(O)=O)(O)=O)([H])N2C3=NC=NC(N)=C3N=C2
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Anal Chim Acta
2024 Nov 15:1329:343235. PMID: 39396298
Solvent & Solubility
H2O : 50 mg/mL (72.72 mM; Need ultrasonic and warming)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Wadler C, et al. Dephospho-CoA kinase provides a rapid and sensitive radiochemical assay for coenzyme A and its thioesters. Anal Biochem. 2007;368(1):17-23. [Content Brief]
[2]. Shimosaka T, et al. Identification of Dephospho-Coenzyme A (Dephospho-CoA) Kinase in Thermococcus kodakarensis and Elucidation of the Entire CoA Biosynthesis Pathway in Archaea. mBio. 2019;10(4):e01146-19. Published 2019 Jul 23. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.4544 mL | 7.2722 mL | 14.5444 mL | 36.3610 mL |
| 5 mM | 0.2909 mL | 1.4544 mL | 2.9089 mL | 7.2722 mL | |
| 10 mM | 0.1454 mL | 0.7272 mL | 1.4544 mL | 3.6361 mL | |
| 15 mM | 0.0970 mL | 0.4848 mL | 0.9696 mL | 2.4241 mL | |
| 20 mM | 0.0727 mL | 0.3636 mL | 0.7272 mL | 1.8180 mL | |
| 25 mM | 0.0582 mL | 0.2909 mL | 0.5818 mL | 1.4544 mL | |
| 30 mM | 0.0485 mL | 0.2424 mL | 0.4848 mL | 1.2120 mL | |
| 40 mM | 0.0364 mL | 0.1818 mL | 0.3636 mL | 0.9090 mL | |
| 50 mM | 0.0291 mL | 0.1454 mL | 0.2909 mL | 0.7272 mL | |
| 60 mM | 0.0242 mL | 0.1212 mL | 0.2424 mL | 0.6060 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Dephospho-CoA Related Classifications
HY-137899 Related Classifications
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