DOI hydrochloride

Name: DOI hydrochloride
Brand: MedChemExpress
SKU: HY-103124
Rating: 4.5 (1 reviews)

Cat. No.: HY-103124 Purity: 99.6%: Data Sheet
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DOI hydrochloride is a serotonin 5-HT_2A/_2C receptor agonist with K_i values of 0.7 nM, 2.4 nM and 20 nM for h5-HT_2A, h5-HT_2C and h5-HT_2B receptors, respectively. DOI hydrochloride can across the blood-brain barrier. DOI hydrochloride increases head twitches in mice.

For research use only. We do not sell to patients.

CAS No. : 42203-78-1

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of DOI hydrochloride:

(R)-DOI hydrochloride In-stock
DOI hydrochloride (Standard) Get quote

1 Publications Citing Use of MCE DOI hydrochloride

•Biomed Pharmacother. 2024 Aug 5:178:117234. [Abstract]

In Vivo Efficacy Study

: DOI hydrochloride purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Aug 5:178:117234. [Abstract]; MT blocks DOI hydrochloride (3 mg/kg, iv, single dose)-induced BP elevation, as shown by systolic BP changes before and after DOI administration under pre-treatment of Vehicle or MT.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

5-HT_2B Receptor

5-HT_2C Receptor

Human 5-HT_2A Receptor

0.7 nM (Ki)

Human 5-HT_2C Receptor

2.4 nM (Ki)

human 5-HT_2B Receptor

20 nM (Ki)

In Vitro

DOI hydrochloride (10 μM; for 45 min) causes a massive increase in adhesion with 99% of the cells staying adherent compared to only 42% of untreated Hu2AAB1 cells^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

DOI hydrochloride (0.3-10 mg/kg; i.p.; single dose) produces an increase in mean head twitches at 3 mg/kg in Wild type (WT) ICR (CD-1) KO mice. Lower doses produces non-significant increases in head twitches, while a higher dose of 10 mg/kg produces increased variability yet a significant increase in mean head twitches^[3].
DOI (0.35-0.7 mmol/kg; sc) significantly increases waking and light sleep and reduces slow wave sleep, rapid-eye-movement (REM) sleep, and the number of REM periods in Male Wistar rats weighing 350-400 g^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

357.62

Formula

C₁₁H₁₇ClINO₂

CAS No.

42203-78-1

Appearance

Solid

Color

White to light yellow

SMILES

NC(C)CC1=CC(OC)=C(I)C=C1OC.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 25 mg/mL (69.91 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7963 mL	13.9813 mL	27.9626 mL
5 mM	0.5593 mL	2.7963 mL	5.5925 mL
10 mM	0.2796 mL	1.3981 mL	2.7963 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

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Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.61%

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Data Sheet (273 KB) SDS (393 KB)

COA (244 KB) HNMR (313 KB) LCMS (253 KB)

Handling Instructions (2659 KB)

References

[1]. D L Nelson, et al. Comparisons of hallucinogenic phenylisopropylamine binding affinities at cloned human 5-HT2A, -HT(2B) and 5-HT2C receptors. Naunyn Schmiedebergs Arch Pharmacol. 1999 Jan;359(1):1-6. [Content Brief]

[2]. S L Shepheard, et al. Investigation of the effects of IVth ventricular administration of the 5-HT2 agonist, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI), on autonomic outflow in the anaesthetized cat. Br J Pharmacol. 1991 Oct;104(2):367-72. [Content Brief]

[3]. Mark J Benvenga, et al. Metabotropic Glutamate 2 Receptors Play a Key Role in Modulating Head Twitches Induced by a Serotonergic Hallucinogen in Mice. Front Pharmacol. 2018 Mar 15;9:208. [Content Brief]

[4]. Jaime M Monti, et al. Effects of the serotonin 5-HT2A/2C receptor agonist DOI and of the selective 5-HT2A or 5-HT2C receptor antagonists EMD 281014 and SB-243213, respectively, on sleep and waking in the rat. Eur J Pharmacol. 2006 Dec 28;553(1-3):163-70. [Content Brief]

[5]. Joe Anand Kumar John Jayakumar, et al. Serotonin 2A (5-HT 2A) receptor affects cell-matrix adhesion and the formation and maintenance of stress fibers in HEK293 cells. Sci Rep. 2020 Dec 10;10(1):21675. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.7963 mL	13.9813 mL	27.9626 mL	69.9066 mL
	5 mM	0.5593 mL	2.7963 mL	5.5925 mL	13.9813 mL
	10 mM	0.2796 mL	1.3981 mL	2.7963 mL	6.9907 mL
	15 mM	0.1864 mL	0.9321 mL	1.8642 mL	4.6604 mL
	20 mM	0.1398 mL	0.6991 mL	1.3981 mL	3.4953 mL
	25 mM	0.1119 mL	0.5593 mL	1.1185 mL	2.7963 mL
	30 mM	0.0932 mL	0.4660 mL	0.9321 mL	2.3302 mL
	40 mM	0.0699 mL	0.3495 mL	0.6991 mL	1.7477 mL
	50 mM	0.0559 mL	0.2796 mL	0.5593 mL	1.3981 mL
	60 mM	0.0466 mL	0.2330 mL	0.4660 mL	1.1651 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.