ICRF-193
Based on 2 publication(s) in Google Scholar
ICRF-193 is a DNA Topoisomerase II inhibitor. (S,S)- and (R,R)-isomers ICRF-193 make up an racemic mixture, ICRF-196 (HY-118590A). ICRF-193 can inhibit DNA syntheses and induces apoptosis. ICRF-193 exhibits anti-cancer and anti-inflammation effects. ICRF-193 shows cardioprotective effect against anthracycline toxicity to cardiomyocytes. ICRF-193 can be used for the researches of cancer, infection, inflammation and cardiovascular disease, such as acute promyelocytic leukemia.
For research use only. We do not sell to patients.
- Purity: 99.64%
- CAS No.: 21416-88-6
- Formula: C12H18N4O4
- Molecular Weight:282.30
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) ICRF-193
MoreAll Topoisomerase Isoforms
MoreAll DNA/RNA Synthesis Isoforms
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Biological Activity
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Topoisomerase II |
ICRF-193 (10-100 μM, 2 h) induces mitotic defects in fission yeast cells expressing histone H2A-RFP, α-tubulin Atb2-GFP and spindle pole body protein Sid4-GFP[1].
ICRF-193 (100 μM. 2 h) inhibits its own induced snapped spindles and induces unequal chromosome segregation and unequal chromosome segregation in fission yeast cells[1].
ICRF-193 (100 μM, 4 h) alters nuclear morphology and DNA content in cdc11-123 fission yeast mutant[1].
ICRF-193 (100 μM, 8 h) reduces cell viability in wild-type fission yeast[1].
ICRF-193 (5 days) inhibits the growth of human acute promyelocytic leukemia (APL) cell lines (NB4, HT-93) and other myeloid leukemia cell lines (HL-60, U937) with IC50 of 0.21-0.26 μM[2].
ICRF-193 (0.1-0.2 μM, 5 days) induces granulocytic differentiation of NB4, HT-93, HL-60, and U937 cells[2].
ICRF-193 (0.2 μM, 48 h) significantly downregulates the level of PML-RAR fusion protein and upregulates p21 and RARβ levels in NB4 cells[2].
ICRF-193 (10 μM) inhibits cells arrested at the quiescent (G0) phase reentry into the S phase and inhibits DNA syntheses in murine spleen cells[3].
ICRF-193 (150 nM, 72 h) inhibits LPS (HY-D1056)-induced IL-1β secretion in human macrophage[4].
ICRF-193 decreases DNA fragmentation induced by Etoposide (HY-13629) and induces apoptosis in murine thymocytes[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 21416-88-6
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Appearance Solid
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Molecular Weight 282.30
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Formula C12H18N4O4
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Color White to off-white
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SMILES
C[C@@H]([C@@H](C)N(CC(N1)=O)CC1=O)N(CC(N2)=O)CC2=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (2)
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Journal Impact Factor
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Most Recent
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Cell
Extrachromosomal DNA replication and maintenance couple with DNA damage pathway in tumors. [Abstract]2025 Jun 26;188(13):3405-3421.e27. PMID: 40300601 -
J Cell Biol
HP1 isoforms direct repair pathway choice in response to heterochromatin double-strand breaks. [Abstract]2026 Mar 2;225(3):e202407146. PMID: 41498760
Solvent & Solubility
DMSO : 2.5 mg/mL (8.86 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
[1]. Nakazawa N, et al. ICRF-193, an anticancer topoisomerase II inhibitor, induces arched telophase spindles that snap, leading to a ploidy increase in fission yeast. Genes Cells. 2016 Sep;21(9):978-93. [Content Brief]
[2]. Niitsu N, et al. The catalytic DNA topoisomerase II inhibitor ICRF-193 and all-trans retinoic acid cooperatively induce granulocytic differentiation of acute promyelocytic leukemia cells: candidate drugs for chemo-differentiation therapy against acute promyelocytic leukemia. Exp Hematol. 2002 Nov;30(11):1273-82. [Content Brief]
[3]. Hossain MS, et al. ICRF-193, a catalytic inhibitor of DNA topoisomerase II, inhibits re-entry into the cell division cycle from quiescent state in mammalian cells. Genes Cells. 2002 Mar;7(3):285-94 [Content Brief]
[4]. Brindle A, et al. The Bisdioxopiperazine ICRF-193 Attenuates LPS-induced IL-1β Secretion by Macrophages. Inflammation. 2024 Feb;47(1):84-98. [Content Brief]
[5]. Jirkovská A, et al. Structure-Activity Relationship Study of Dexrazoxane Analogues Reveals ICRF-193 as the Most Potent Bisdioxopiperazine against Anthracycline Toxicity to Cardiomyocytes Due to Its Strong Topoisomerase IIβ Interactions. J Med Chem. 2021 Apr 8;64(7):3997-4019. [Content Brief]
[6]. Tanimoto C, et al. ICRF-193 modifies etoposide-induced apoptosis in thymocytes. Acta Med Okayama. 1995 Dec;49(6):281-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5423 mL | 17.7117 mL | 35.4233 mL | 88.5583 mL |
| 5 mM | 0.7085 mL | 3.5423 mL | 7.0847 mL | 17.7117 mL |
ICRF-193 Related Classifications
HY-118590 Related Classifications
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