Estradiol 3-methyl ether
Based on 1 Customer Validation
Estradiol 3-methyl ether (EDME) is a highly selective TRPML1 ion channel antagonist and microtubule (microtubule) depolymerizing agent, with IC50 values of 0.22 μM and 3.8 μM against TRPML1 and TRPML2, respectively; it shows no activity against TRPML3. Estradiol 3-methyl ether induces the disruption of cytoplasmic microtubule networks in mammalian cells, with an EC50 of 9 μM. Independent of estrogen receptors, Estradiol 3-methyl ether blocks autophagy (autophagy), TFEB nuclear translocation, and inhibits the migration and invasion of triple-negative breast cancer cells by suppressing TRPML1. Estradiol 3-methyl ether is applicable for relevant research on triple-negative breast cancer.
For research use only. We do not sell to patients.
- Purity: 99.07%
- CAS No.: 1035-77-4
- Formula: C19H26O2
- Molecular Weight:286.41
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Estradiol 3-methyl ether (1 μM; 3 h) inhibits TFEB nuclear translocation and blocks the induction of autophagy[1].
Estradiol 3-methyl ether (0.1-10 μM) significantly inhibits the invasive ability of MDA-MB-231 WT cells in a dose-dependent manner[1].
Estradiol 3-methyl ether (9-100 μM; 1 h) induces microtubule breakage in Chinese hamster V79 cells, with an EC50 of 9 μM after 1 h of incubation, and completely abolishes the normal microtubule network at a concentration of 100 μM[2].
Estradiol 3-methyl ether (20-50 μM; 2-4 days) inhibits the growth of Chinese hamster V79 cells, and treatment with 20 μM for 2 days causes 92% growth inhibition[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Chinese hamster V79 cells
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Concentration:20 μM (2-day incubation); 50 μM (4-day incubation)
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Incubation Time:2 days (20 μM); 4 days (50 μM)
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Result:Caused 92% inhibition of cell growth at 20 μM after 2 days of treatment.
Exhibited more potent cell growth-inhibitory activity than 17β-estradiol (HY-B0141) at 50 μM over 4 days of treatment.
Chemical Information
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CAS No. 1035-77-4
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Appearance Solid
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Molecular Weight 286.41
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Formula C19H26O2
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Color White to off-white
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SMILES
C[C@]12CC[C@]3([H])C4=CC=C(OC)C=C4CC[C@@]3([H])[C@]1([H])CC[C@]2([H])O
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Synonyms
3-O-Methyl estradiol; 17β-Estradiol 3-methyl ether; 3-Methoxyestradiol
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (349.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Rühl P, et al. Estradiol analogs attenuate autophagy, cell migration and invasion by direct and selective inhibition of TRPML1, independent of estrogen receptors. Sci Rep. 2021;11(1):8313. Published 2021 Apr 15. [Content Brief]
[2]. Aizu-Yokota E, et al. Natural estrogens induce modulation of microtubules in Chinese hamster V79 cells in culture. Cancer Res. 1995;55(9):1863-1868. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4915 mL | 17.4575 mL | 34.9150 mL | 87.2875 mL |
| 5 mM | 0.6983 mL | 3.4915 mL | 6.9830 mL | 17.4575 mL | |
| 10 mM | 0.3491 mL | 1.7457 mL | 3.4915 mL | 8.7287 mL | |
| 15 mM | 0.2328 mL | 1.1638 mL | 2.3277 mL | 5.8192 mL | |
| 20 mM | 0.1746 mL | 0.8729 mL | 1.7457 mL | 4.3644 mL | |
| 25 mM | 0.1397 mL | 0.6983 mL | 1.3966 mL | 3.4915 mL | |
| 30 mM | 0.1164 mL | 0.5819 mL | 1.1638 mL | 2.9096 mL | |
| 40 mM | 0.0873 mL | 0.4364 mL | 0.8729 mL | 2.1822 mL | |
| 50 mM | 0.0698 mL | 0.3491 mL | 0.6983 mL | 1.7457 mL | |
| 60 mM | 0.0582 mL | 0.2910 mL | 0.5819 mL | 1.4548 mL | |
| 80 mM | 0.0436 mL | 0.2182 mL | 0.4364 mL | 1.0911 mL | |
| 100 mM | 0.0349 mL | 0.1746 mL | 0.3491 mL | 0.8729 mL |
Estradiol 3-methyl ether Related Classifications
HY-W008581 Related Classifications
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- Estradiol 3-methyl ether
- 1035-77-4
- 3-O-Methyl estradiol
- 17β-Estradiol 3-methyl ether
- 3-Methoxyestradiol
- Biochemical Assay Reagents
- TRP Channel
- Microtubule/Tubulin
- Autophagy
- TPC2
- triple-negative breast cancer cells
- TRPML1 channel
- TFEB
- Chinese hamster V79 cells
- estrogen receptor alpha
- mammalian cells
- TRPML2
- TRPML3
- human ERα
- Inhibitor
- inhibitor
- inhibit