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Cat. No.: HY-D3169

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IETDC

IETDC is a caspase 8-responsive fluorescent probe. IETDC is cleaved by activated caspase 8 to release D-cysteine, which then reacts with 6-hydroxy-2-cyanobenzothiazole (HCBT) to form firefly luciferin in situ. IETDC must be used in conjunction with PCL-2 (HY-D3168) to detect the co-existence of hydrogen peroxide and caspase 8. IETDC is applicable to studies related to acute inflammation.

For research use only. We do not sell to patients.

CAS No.: 1476735-95-1
Formula: C₃₂H₄₇N₅O₁₃S
Molecular Weight:741.81

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Custom Peptide Synthesis

IETDC Chemical Structure

CAS No.: 1476735-95-1

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References
Calculators
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Biological Activity

Description

IC₅₀ & Target

^[1]

Caspase-8

All Caspase Isoforms :

In Vitro

IETDC (5 μM; 60 min) is selectively cleaved by recombinant caspase 8 but not caspase 3 or caspase 9 to release D-cysteine, which forms luciferin with HCBT to produce a ca. 27-fold increase in bioluminescent signal, and this response is fully inhibited by a pan-caspase inhibitor^[1].

IETDC (10 μM; 60 min) acts as part of an AND-type molecular logic gate, producing an ca. 18-fold increase in bioluminescent signal only when combined with PCL-2, hydrogen peroxide, and active caspase 8, with signal blocked by inhibition of either hydrogen peroxide-mediated PCL-2 cleavage or caspase 8-mediated IETDC cleavage^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Mice

IETDC (0.05 μmol; i.p.; single dose), co-administered with HCBT, produces an 18-fold bioluminescent signal increase in female FVB-luc+ mice with lipopolysaccharide-induced acute inflammation, which is 34% attenuated by pre-treatment with a pan-caspase inhibitor^[1].

Mice

IETDC (0.05 μmol; i.p.; single dose), co-administered with PCL-2, produces a 2.7-fold bioluminescent signal increase in female FVB-luc+ mice with LPS (HY-D1056)-induced acute inflammation, which is 30% attenuated by co-treatment with ascorbic acid and a pan-caspase inhibitor^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

FVB-luc+ (female, 2-5 months old)^[1]
Dosage:

0.05 μmol (co-administered with 0.05 μmol HCBT)
Administration:

i.p.; single dose
Result:

Produced an 18-fold increase in bioluminescent signal in lipopolysaccharide-treated mice compared to saline-treated controls.
Caused a 34% attenuation of the elevated bioluminescent signal when pre-treated with z-VD(OMe)-OPh.

Animal Model:

FVB-luc+ (female, 2-5 months old)^[1]
Dosage:

0.05 μmol (co-administered with 0.05 μmol PCL-2)
Administration:

i.p.; single dose
Result:

Produced a 2.7-fold increase in bioluminescent signal in lipopolysaccharide-treated mice compared to saline-treated controls.
Caused a 30% attenuation of the elevated bioluminescent signal when co-treated with ascorbic acid and z-VD(OMe)-OPh.

Related Protocols :

Chemical Information

CAS No. 1476735-95-1
Molecular Weight 741.81
Formula C₃₂H₄₇N₅O₁₃S
Sequence

Cbz-Ile-{Glu(Me)}-Thr-{Asp(Me)}-{d-Cys}
Sequence Shortening

Cbz-I-{Glu(Me)}-T-{Asp(Me)}-{d-Cys}
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Van de Bittner GC, et al. Strategy for dual-analyte luciferin imaging: in vivo bioluminescence detection of hydrogen peroxide and caspase activity in a murine model of acute inflammation. J Am Chem Soc. 2013;135(5):1783-1795. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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