2-5A
2-5A (2′,5′-ApApA) is a 5'-triphosphorylated (2',5') oligoadenylate. When covalently linked to an antisense molecule complementary to the target sequence, 2-5A activates RNase L, which subsequently degrades the target RNA bound to the 2-5A chimera. 2-5A can be used in research related to respiratory syncytial virus infection, colorectal adenocarcinoma and melanoma.
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- No. CAS: 65954-93-0
- Fòrmula: C30H40N15O25P5
- Peso molecular:1165.59
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
Descripciòn
IC50 & Target
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RNASE L |
In Vitro
2-5A (1 μM; 4 h) is imported by human HEK293T cells and BJ1 fibroblasts to activate RNase L-dependent IFN/ISG induction, while mouse MEFs, MC38 cells, BMDMs, human A549 cells, and THP-1 monocytes do not exhibit robust 2-5A import activity[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human HEK293T cells, human BJ1 fibroblasts, mouse MEFs, mouse MC38 cells, mouse bone-marrow-derived macrophages (BMDMs), human A549 cells, human THP-1 monocytes
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Concentration:1 μM
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Incubation Time:4 h
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Result:Stimulated significant IFNB1 and CXCL10 mRNA expression in human HEK293T cells and BJ1 fibroblasts.
Abolished IFN/ISG response in RNase L-knockout HEK293T and BJ1 cells.
Failed to induce robust IFN/ISG response in mouse MEFs, MC38 cells, BMDMs, human A549 cells, and THP-1 monocytes.
Induced robust IFN/ISG expression in all tested cell lines when transfected with Lipofectamine.
In Vivo
2-5A (10 μg; peritumoral injection; once daily; 3 consecutive days) complexed with lipofectamine significantly inhibits B16 melanoma tumor growth in wild-type C57BL/6J mice[3].
2-5A (10 μg; peritumoral injection; once daily; 3 consecutive days) complexed with lipofectamine significantly inhibits sgRnasel MC38 colorectal carcinoma tumor growth in wild-type C57BL/6J mice[3].
2-5A (10 μg; peritumoral injection; once daily; 3 consecutive days) complexed with lipofectamine does not inhibit MC38 colorectal carcinoma tumor growth in RnaseI−/− mice[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6J wild-type (6-8 weeks old, same sex, subcutaneous implantation of 5×105 WT MC38 cells)[3]
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Dosage:10 μg (complexed with lipofectamine)
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Administration:peritumoral injection; once daily; 3 consecutive days
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Result:Significantly inhibited MC38 tumor growth compared to PBS, naked 2-5A (TEA), or lipofectamine alone.
Showed statistically significant difference in tumor volume between the lipofectamine+2-5A group and lipofectamine alone group (P=0.0142).
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Animal Model:C57BL/6J wild-type (6-8 weeks old, same sex, subcutaneous implantation of 5×105 B16 cells)[3]
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Dosage:10 μg (complexed with lipofectamine)
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Administration:peritumoral injection; once daily; 3 consecutive days
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Result:Significantly inhibited B16 melanoma tumor growth compared to lipofectamine alone (P=0.0185).
Chemical Information
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No. CAS 65954-93-0
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Peso molecular 1165.59
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Fòrmula C30H40N15O25P5
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SMILES
NC1=NC=NC2=C1N=CN2[C@H]3[C@H](OP(O)(OC[C@@H](O[C@H]([C@@H]4OP(O)(OC[C@@H](O[C@H]([C@@H]5O)N6C=NC7=C6N=CN=C7N)[C@H]5O)=O)N8C=NC9=C8N=CN=C9N)[C@H]4O)=O)[C@H](O)[C@@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)O3
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Synonyms
2′,5′-ApApA; 2′,5′-trioligoadenylate; 5'-O-Triphosphoryladenylyl-(2'→5')-adenylyl-(2'→5')-adenosine
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
[1]. Barnard DL, et al. 2-5A-DNA conjugate inhibition of respiratory syncytial virus replication: effects of oligonucleotide structure modifications and RNA target site selection. Antiviral research. 1999 Apr;41(3):119-34. [Content Brief]
[2]. Wang S, et al. 2-5A is an immunotransmitter that fuels RNase L immunity. Immunity. 2025 Apr 08;58(4):770-772. [Content Brief]
[3]. Huai W, et al. OAS cross-activates RNase L intercellularly through cell-to-cell transfer of 2-5A to spread innate immunity. Immunity. 2025 Apr 08;58(4):797-810.e6. [Content Brief]
[4]. Kalinichenko EN, et al. 3'-Fluoro-3'-deoxy analogs of 2-5A 5'-monophosphate: binding to 2-5A-dependent endoribonuclease and susceptibility to (2'-5')phosphodiesterase degradation. Biochemical and biophysical research communications. 1990 Feb 28;167(1):20-6. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
2-5A Related Classifications
HY-173189 Related Classifications
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Keywords
- 2-5A
- 65954-93-0
- 2′,5′-ApApA
- 2′,5′-trioligoadenylate
- 5'-O-Triphosphoryladenylyl-(2'→5')-adenylyl-(2'→5')-adenosine
- RSV
- DNA/RNA Synthesis
- RNase L
- Sendai virus
- HEK293T human embryonic kidney cells
- BJ1 human foreskin fibroblasts
- ENPP1
- MC38 cells
- melanoma
- vesicular stomatitis virus
- colorectal adenocarcinoma
- respiratory syncytial virus
- Inhibitor
- inhibitor
- inhibit