Aloin-A
Based on 8 publication(s) in Google Scholar
Aloin (Aloin-A; Barbaloin-A) is a natural anti-tumor anthraquinone glycoside with iron chelating activity. Aloin induces the differentiation of MC3T3-E1 cells into osteoblasts through MAPK-mediated Wnt and Bmp signaling pathways. Alkaline phosphatase (ALP) is an early marker of osteoblast differentiation, and the activity of ALP is also enhanced by Aloin. Aloin also reduces brain edema, reduces blood-brain barrier disruption and improves cortical impact injuries. Aloin is used in research into osteoporosis and traumatic brain injury (TBI).
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- Pureza: 99.31%
- No. CAS: 1415-73-2
- Fòrmula: C21H22O9
- Peso molecular:418.39
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Aloin-A
More- Chin Med. 2021 Nov 24;16(1):123. [Abstract]
- Qual Assur Saf Crop. 2022; 14(2): 58–65.
- Arab J Chem. 2020 Feb.
- Int Immunopharmacol. 2020 Dec;89(Pt B):107079. [Abstract]
- Langmuir. 2025 Nov 11;41(44):29688-29708. [Abstract]
- Sci Rep. 2022 Feb 9;12(1):2145. [Abstract]
- Stem Cells Int. 2022 Jan 6:2022:5865011. [Abstract]
- University of Paris. 2022 Sep 19.
Actividad biológica
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Vero C1008 | CC50 |
>100 μM
Compound: 42
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Cytotoxicity against African green monkey Vero E6 cells assessed as cell viability treated for 24 to 48 hrs by prestoblue staining based assay
Cytotoxicity against African green monkey Vero E6 cells assessed as cell viability treated for 24 to 48 hrs by prestoblue staining based assay
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[PMID: 35620927] |
Aloin (0.01-1 μM; 10 d) enhances the expression of osteoblast differentiation genes, Bmp-2, Runx2, and collagen 1a in a dose-dependent manner[3].
Aloin (0.05 μM; 10 d) induces the differentiation of MC3T3-E1 cells into osteoblasts through MAPK-mediated Wnt and Bmp signaling pathways[3].
Aloin (0.05 μM; 10 d) increases ALP activity in MC3T3-E1 cells[3].
Aloin (10-80 μg/mL; 4.5 h) increases cell viability after biaxial stretch injury (SI), via attenuates intracellular ROS generation, inhibits changes of mitochondrial membrane potentials in mouse cerebrovascular endothelial cells (ECs)[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 1415-73-2
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Appearance Solid
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Peso molecular 418.39
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Fòrmula C21H22O9
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Color Light yellow to yellow
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SMILES
O=C1C2=C(C=CC=C2O)[C@H]([C@]3([H])O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)C4=CC(CO)=CC(O)=C14
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Synonyms
Barbaloin-A
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (8)
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Journal Impact Factor
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Most Recent
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Chin Med
Aloin induced apoptosis by enhancing autophagic flux through the PI3K/AKT axis in osteosarcoma. [Abstract]2021 Nov 24;16(1):123. PMID: 34819120 -
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Int Immunopharmacol
Inhibition of PI3K/Akt/NF-κB signaling by Aloin for ameliorating the progression of osteoarthritis: In vitro and in vivo studies. [Abstract]2020 Dec;89(Pt B):107079. PMID: 33096361 -
Langmuir
2025 Nov 11;41(44):29688-29708. PMID: 41169226 -
Sci Rep
Aloin isoforms (A and B) selectively inhibits proteolytic and deubiquitinating activity of papain like protease (PLpro) of SARS-CoV-2 in vitro. [Abstract]2022 Feb 9;12(1):2145. PMID: 35140265 -
Stem Cells Int
Aloin Regulates Matrix Metabolism and Apoptosis in Human Nucleus Pulposus Cells via the TAK1/NF- κ B/NLRP3 Signaling Pathway. [Abstract]2022 Jan 6:2022:5865011. PMID: 35035490 -
Solvente y solubilidad
DMSO : 116.67 mg/mL (278.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.97 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.97 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (284 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Pan Q, et al. Inhibition of the angiogenesis and growth of Aloin in human colorectal cancer in vitro and in vivo. Cancer Cell Int. 2013 Jul 12;13(1):69. [Content Brief]
[2]. Tabolacci C, et al. Aloin enhances cisplatin antineoplastic activity in B16-F10 melanoma cells by transglutaminase-induced differentiation. Amino Acids. 2013 Jan;44(1):293-300. [Content Brief]
[3]. Cui Y, et al. Aloin protects against chronic alcoholic liver injury via attenuating lipid accumulation, oxidative stress and inflammation in mice. Arch Pharm Res. 2014 Dec;37(12):1624-33. [Content Brief]
[4]. Pengjam Y, et al. Anthraquinone Glycoside Aloin Induces Osteogenic Initiation of MC3T3-E1 Cells: Involvement of MAPK Mediated Wnt and Bmp Signaling. Biomol Ther (Seoul). 2016 Mar 1;24(2):123-3 [Content Brief]
[5]. Jing Y, et al. Aloin Protects Against Blood-Brain Barrier Damage After Traumatic Brain Injury in Mice. Neurosci Bull. 2020 Jun;36(6):625-638. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3901 mL | 11.9506 mL | 23.9011 mL | 59.7529 mL |
| 5 mM | 0.4780 mL | 2.3901 mL | 4.7802 mL | 11.9506 mL | |
| 10 mM | 0.2390 mL | 1.1951 mL | 2.3901 mL | 5.9753 mL | |
| 15 mM | 0.1593 mL | 0.7967 mL | 1.5934 mL | 3.9835 mL | |
| 20 mM | 0.1195 mL | 0.5975 mL | 1.1951 mL | 2.9876 mL | |
| 25 mM | 0.0956 mL | 0.4780 mL | 0.9560 mL | 2.3901 mL | |
| 30 mM | 0.0797 mL | 0.3984 mL | 0.7967 mL | 1.9918 mL | |
| 40 mM | 0.0598 mL | 0.2988 mL | 0.5975 mL | 1.4938 mL | |
| 50 mM | 0.0478 mL | 0.2390 mL | 0.4780 mL | 1.1951 mL | |
| 60 mM | 0.0398 mL | 0.1992 mL | 0.3984 mL | 0.9959 mL | |
| 80 mM | 0.0299 mL | 0.1494 mL | 0.2988 mL | 0.7469 mL | |
| 100 mM | 0.0239 mL | 0.1195 mL | 0.2390 mL | 0.5975 mL |
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