DM4
Based on 6 publication(s) in Google Scholar
DM4 is is an antitubulin agent that inhibit cell division. DM4 can be used in the preparation of antibody agent conjugate.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.64%
- No. CAS: 796073-69-3
- Fòrmula: C38H54ClN3O10S
- Peso molecular:780.37
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) DM4
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Customer Validation & Images
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Cell Proliferation/Viability Assay
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Flow Cytometry
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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Cell Proliferation/Viability Assay
Ver todos los productos específicos de isoformas ADC Payloads
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Actividad biológica
DM4, a structural analogue of maytansine, is a new thiol-containing and potent maytansinoid. DM4 is a cytotoxic maytansinoid drug. It is synthesized in order to link maytansinoids to antibodies via disulfide bonds. Maytansinoids inhibit tubulin polymerization and microtubule assembly and enhance microtubule destabilization, so there is potent suppression of microtubule dynamics resulting in a mitotic block and subsequent apoptotic cell death[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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No. CAS 796073-69-3
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Appearance Solid
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Peso molecular 780.37
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Fòrmula C38H54ClN3O10S
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Color White to light yellow
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SMILES
C[C@]1([C@H](CC(N(C(C=C2C=C3OC)=C3Cl)C)=O)OC([C@H](C)N(C)C(CCC(C)(S)C)=O)=O)[C@H]([C@@H]([C@](OC4=O)([H])C[C@]([C@](/C=C/C=C(C)/C2)([H])OC)(N4)O)C)O1
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Synonyms
Ravtansine
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (6)
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Journal Impact Factor
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Most Recent
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J Transl Med
LGALS3BP antibody-drug conjugate enhances tumor-infiltrating lymphocytes and synergizes with immunotherapy to restrain neuroblastoma growth. [Abstract]2025 Apr 11;23(1):431. PMID: 40217513
DM4 purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Apr 11;23(1):431. [Abstract]
In vitro IC50 curves were assessed through cytotoxicity assays by exposing NXS2 CTRL and hLGALS3BP cells to increasing doses of payload DM4 (0.00064 to 250 nM; 72 h).
DM4 purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Apr 11;23(1):431. [Abstract]
Surface levels of PD-L1 evaluated through FACS analysis expressed as fold change of mean fluorescence intensity signals of treated samples over controls of NXS2 control and hLGALS3BP cells treated with DM4 (3 nM; 72 h) or with DMA. DM4 has the ability to induce a potential resistance mechanism.
DM4 purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Apr 11;23(1):431. [Abstract]
1959-sss/DM4 (ADC) (10 mg/kg; twice weekly; three total i.v.) treatment resulted in a significant shrinkage of hLGALS3BP-expressing tumors in syngeneic A/J mice.
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ACS Omega
Evaluation of Antibody-Drug Conjugate Performances Using a Novel HPLC-DAD Method for Tumor-Specific Detection of DM4 and S‑Methyl-DM4. [Abstract]2025 Oct 15;10(42):50382-50393. PMID: 41179189
DM4 purchased from MedChemExpress. Usage Cited in: ACS Omega. 2025 Oct 15;10(42):50382-50393. [Abstract]
1959-sss/DM4 (ADC) (10 mg/kg; twice weekly; three total i.v.) significantly reduced metastatic lesion area, especially in mice injected with LGALS3BP-expressing hNB cells.
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Curr Issues Mol Biol
Targeting GD2-Positive Tumor Cells by Pegylated scFv Fragment-Drug Conjugates Carrying Maytansinoids DM1 and DM4. [Abstract]2023 Oct 5;45(10):8112-8125. PMID: 37886955
DM4 purchased from MedChemExpress. Usage Cited in: Curr Issues Mol Biol. 2023 Oct 5;45(10):8112-8125. [Abstract]
Cytotoxic activity of pegylated antibody fragment-drug conjugates in GD2-positive B78-D14 cell line. scFv-PEG4-DM4 (0-370 nM; 72 h) was more effective than scFv-PEG4-DM1 in inhibiting cell growth because the cytotoxicity was approximately twice as high for the FDC loaded with DM4 as opposed to that with DM1.
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J Pharm Biomed Anal
HPLC-DAD validated method for DM4 and its metabolite S-Me-DM4 quantification in biological matrix for clinical and pharmaceutical applications. [Abstract]2023 Oct 25:235:115642. PMID: 37586307 -
Oral Dis
LGALS3BP is a potential target of antibody-drug conjugates in oral squamous cell carcinoma. [Abstract]2024 May;30(4):2039-2050. PMID: 37649401 -
Solvente y solubilidad
DMSO : 100 mg/mL (128.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.20 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.20 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (275 KB)
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SDS (643 KB)
- English - EN (643 KB)
- Français - FR (643 KB)
- Deutsch - DE (643 KB)
- Norwegian - NO (643 KB)
- Español - ES (643 KB)
- Swedish - SV (643 KB)
- Italian - IT (643 KB)
- Korean - KR (643 KB)
- Portuguese - PT (643 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.2814 mL | 6.4072 mL | 12.8144 mL | 32.0361 mL |
| 5 mM | 0.2563 mL | 1.2814 mL | 2.5629 mL | 6.4072 mL | |
| 10 mM | 0.1281 mL | 0.6407 mL | 1.2814 mL | 3.2036 mL | |
| 15 mM | 0.0854 mL | 0.4271 mL | 0.8543 mL | 2.1357 mL | |
| 20 mM | 0.0641 mL | 0.3204 mL | 0.6407 mL | 1.6018 mL | |
| 25 mM | 0.0513 mL | 0.2563 mL | 0.5126 mL | 1.2814 mL | |
| 30 mM | 0.0427 mL | 0.2136 mL | 0.4271 mL | 1.0679 mL | |
| 40 mM | 0.0320 mL | 0.1602 mL | 0.3204 mL | 0.8009 mL | |
| 50 mM | 0.0256 mL | 0.1281 mL | 0.2563 mL | 0.6407 mL | |
| 60 mM | 0.0214 mL | 0.1068 mL | 0.2136 mL | 0.5339 mL | |
| 80 mM | 0.0160 mL | 0.0801 mL | 0.1602 mL | 0.4005 mL | |
| 100 mM | 0.0128 mL | 0.0641 mL | 0.1281 mL | 0.3204 mL |
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