Demethoxycurcumin
Based on 6 publication(s) in Google Scholar
Demethoxycurcumin is one of the main active ingredients of curcumin, which has anti-inflammatory, antioxidant, antibacterial, anti-cancer and neuroprotective effects. Demethoxycurcumin is orally active. Demethoxycurcumin can be used in inflammation, cancer and Alzheimer's disease research.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.95%
- No. CAS: 22608-11-3
- Fòrmula: C20H18O5
- Peso molecular:338.36
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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HY-N0005
Curcumin -
HY-N0007
(E,E)-Bisdemethoxycurcumin -
HY-N0007A
Bisdemethoxycucurmin -
HY-N0102
Isoliquiritigenin -
HY-N0893
Tetrahydrocurcumin -
HY-N0006R
Demethoxycurcumin (Standard) -
HY-N0155
Nobiletin -
HY-N0822
Shikonin -
HY-15097
Myricetin -
HY-N0005R
Curcumin (Standard) -
HY-N0290
Mangiferin -
HY-15194
Dimethylcurcumin
Publications Citing Use of MedChemExpress (MCE) Demethoxycurcumin
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Customer Validation & Images
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Cell Proliferation/Viability Assay
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WB
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IP
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IHC
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Histological Imaging/Staining
Actividad biológica
Descripciòn
IC50 & Target
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ChAT |
iNOS |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A-431 | IC50 |
>100 μM
Compound: DC
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Cytotoxicity against human A431 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay
Cytotoxicity against human A431 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay
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[PMID: 29605808] |
| A549 | IC50 |
28.2 μM
Compound: DC
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Cytotoxicity against human A549 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay
Cytotoxicity against human A549 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay
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[PMID: 29605808] |
| DLD-1 | IC50 |
>40 μM
Compound: DC
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Cytotoxicity against human DLD1 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay
Cytotoxicity against human DLD1 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay
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[PMID: 29605808] |
| HT-1080 | IC50 |
7.5 μM
Compound: DMC
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Decrease in UPA expression in human HT1080 cells
Decrease in UPA expression in human HT1080 cells
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[PMID: 31336310] |
| MCF7 | IC50 |
92.1 μM
Compound: DC
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Cytotoxicity against human MCF7 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay
Cytotoxicity against human MCF7 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay
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[PMID: 29605808] |
| MDA-MB-231 | IC50 |
9 mM
Compound: DMC
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Increase in MMP3 level in human MDA-MB-231 cells after 24 hrs by ELISA
Increase in MMP3 level in human MDA-MB-231 cells after 24 hrs by ELISA
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[PMID: 31336310] |
| WRL68 | IC50 |
26.3 μM
Compound: DC
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Cytotoxicity against human WRL68 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay
Cytotoxicity against human WRL68 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay
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[PMID: 29605808] |
In Vitro
Demethoxycurcumin (0-150 μM, 0-96 h) exerts anti-inflammatory effect on Caco-2 cells of in vitro inflamed human intestinal mucosa model by inhibiting NO secretion induced by iNOS[1].
Demethoxycurcumin (35 μM, 48 h) can change the expression of DNA damage, cell cycle and apoptosis-related genes in human lung cancer NCI-H460 cells, and exhibit anticancer activity[2].
The IC50 values of Demethoxycurcumin (2-1024 μg/mL, 12-14 h) forE. coli, S. aureus, and S. dysenteriae are 212.5, 282.5 and 465.0 μg/mL, and the MIC values are 512, 1024 and 1024 μg/mL, respectively[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Caco-2 Cells (LPS-induced inflammation model cell)
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Concentration:0-150 μM
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Incubation Time:0-96 h
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Result:Showed cytotoxic effects on CACO-2-differentiated cells at 130 μM and 150 μM concentrations.
In Vivo
Demethoxycurcumin (10 mg/kg/day for 28 days, p.o.) can enhance cognition and enhance choline acetyltransferase (ChAT) activity in mice induced by Scopolamine (HY-N0296) [4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male ICR mice (6 weeks old) weighing 30–35 g, memory impairment induced by Scopolamine[5]
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Dosage:10 mg/kg/day for 28 days
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Administration:Oral gavage (p.o.)
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Result:Significantly improved scopolamine induced passive avoidance task learning disabilities and memory impairments in Morris Water Maze.
The activity of choline acetyltransferase (ChAT) was significantly increased to 33.03%.
Chemical Information
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No. CAS 22608-11-3
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Appearance Solid
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Peso molecular 338.36
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Fòrmula C20H18O5
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Color Pink to red
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SMILES
O=C(CC(/C=C/C1=CC=C(O)C=C1)=O)/C=C/C2=CC=C(O)C(OC)=C2
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Synonyms
Curcumin II; Desmethoxycurcumin; Monodemethoxycurcumin
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (6)
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Journal Impact Factor
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Most Recent
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Life Sci
Salvianolic acid A inhibits tumor cell proliferation and induces chromosomal abnormalities by blocking the citrullinating enzyme PADs. [Abstract]2026 Aug 1:398:124444. PMID: 42107692 -
Cancer Gene Ther
2025 Oct 2. PMID: 41039015
Demethoxycurcumin purchased from MedChemExpress. Usage Cited in: Cancer Gene Ther. 2025 Oct 2. [Abstract]
Cell viability was measured by MTS assay in CAL27 treated with Demethoxycurcumin (Deme) (0, 2, 4, 8 μM).
Demethoxycurcumin purchased from MedChemExpress. Usage Cited in: Cancer Gene Ther. 2025 Oct 2. [Abstract]
IB analysis of HK2 and HK1 levels in Demethoxycurcumin (Deme) (0, 2, 4, 8 μM)-treated CAL27 and SCC25 cells.
Demethoxycurcumin purchased from MedChemExpress. Usage Cited in: Cancer Gene Ther. 2025 Oct 2. [Abstract]
CAL27 cells were treated with Demethoxycurcumin (Deme) (8 μM) for 24 h by subsequent IP assay with USP13 or PTEN antibodies.
Demethoxycurcumin purchased from MedChemExpress. Usage Cited in: Cancer Gene Ther. 2025 Oct 2. [Abstract]
IHC staining treated with Demethoxycurcumin (Deme) (10 mg/kg, i.p.).
Demethoxycurcumin purchased from MedChemExpress. Usage Cited in: Cancer Gene Ther. 2025 Oct 2. [Abstract]
H&E staining of major organs from tumor-bearing mice with vehicle or Demethoxycurcumin (Deme) (10 mg/kg, i.p.) treatment.
Demethoxycurcumin purchased from MedChemExpress. Usage Cited in: Cancer Gene Ther. 2025 Oct 2. [Abstract]
Demethoxycurcumin (Deme) (10 mg/kg, i.p.) inhibits tumor growth in vivo.
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Philos Trans R Soc Lond B Biol Sci
2023 Nov 20;378(1890):20220248. PMID: 37778388 -
Vet Microbiol
Curcumin inhibits classical swine fever virus replication by interfering with lipid metabolism. [Abstract]2021 Aug:259:109152. PMID: 34146894 -
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (295.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3.25 mg/mL (9.61 mM); Clear solution
This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (276 KB)
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SDS (459 KB)
- English - EN (459 KB)
- Français - FR (459 KB)
- Deutsch - DE (459 KB)
- Norwegian - NO (459 KB)
- Español - ES (459 KB)
- Swedish - SV (459 KB)
- Italian - IT (459 KB)
- Korean - KR (459 KB)
- Portuguese - PT (459 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Somchit M, et al. Demethoxycurcumin from Curcuma longa rhizome suppresses iNOS induction in an in vitro inflamed human intestinal mucosa model. Asian Pac J Cancer Prev. 2014;15(4):1807-10. [Content Brief]
[2]. Ko YC, et al. Demethoxycurcumin Alters Gene Expression Associated with DNA Damage, Cell Cycle and Apoptosis in Human Lung Cancer NCI-H460 Cells In Vitro. In Vivo. 2015 01-02;29(1):83-94. [Content Brief]
[3]. Ahmed T, et al. A comparative study of curcuminoids to measure their effect on inflammatory and apoptotic gene expression in an Aβ plus ibotenic acid-infused rat model of Alzheimer's disease. Brain Res. 2011 Jul 11;1400:1-18. [Content Brief]
[4]. Lim DW, et al. Enhanced Cognitive Effects of Demethoxycurcumin, a Natural Derivative of Curcumin on Scopolamine-Induced Memory Impairment in Mice. Molecules. 2016 Aug 5;21(8):1022. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9554 mL | 14.7772 mL | 29.5543 mL | 73.8858 mL |
| 5 mM | 0.5911 mL | 2.9554 mL | 5.9109 mL | 14.7772 mL | |
| 10 mM | 0.2955 mL | 1.4777 mL | 2.9554 mL | 7.3886 mL | |
| 15 mM | 0.1970 mL | 0.9851 mL | 1.9703 mL | 4.9257 mL | |
| 20 mM | 0.1478 mL | 0.7389 mL | 1.4777 mL | 3.6943 mL | |
| 25 mM | 0.1182 mL | 0.5911 mL | 1.1822 mL | 2.9554 mL | |
| 30 mM | 0.0985 mL | 0.4926 mL | 0.9851 mL | 2.4629 mL | |
| 40 mM | 0.0739 mL | 0.3694 mL | 0.7389 mL | 1.8471 mL | |
| 50 mM | 0.0591 mL | 0.2955 mL | 0.5911 mL | 1.4777 mL | |
| 60 mM | 0.0493 mL | 0.2463 mL | 0.4926 mL | 1.2314 mL | |
| 80 mM | 0.0369 mL | 0.1847 mL | 0.3694 mL | 0.9236 mL | |
| 100 mM | 0.0296 mL | 0.1478 mL | 0.2955 mL | 0.7389 mL |
Demethoxycurcumin Related Classifications
HY-N0006 Related Classifications
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