Geraniin
Based on 3 publication(s) in Google Scholar
Geraniin is a TNF-α releasing inhibitor with numerous activities including anticancer, anti-inflammatory, and anti-hyperglycemic activities, with an IC50 of 43 μM.
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- Pureza: 99.82%
- No. CAS: 60976-49-0
- Fòrmula: C41H28O27
- Peso molecular:952.64
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Almacenamiento:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Geraniin
MoreVer todos los productos específicos de isoformas TNF Receptor
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Actividad biológica
IC50: 43 μM (TNF-α)[1].
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | ED50 |
>10 μg/mL
Compound: 2
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Cytotoxicity against human A549 cells by tetrazolium salt-based colorimetric assay
Cytotoxicity against human A549 cells by tetrazolium salt-based colorimetric assay
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[PMID: 1431932] |
| HCT-8 | ED50 |
>10 μg/mL
Compound: 2
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Cytotoxicity against human HCT8 cells by tetrazolium salt-based colorimetric assay
Cytotoxicity against human HCT8 cells by tetrazolium salt-based colorimetric assay
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[PMID: 1431932] |
| Huh-7 | CC50 |
63.4 μM
Compound: 22
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Cytotoxicity against human Huh7.5 cells after 96 hrs by MTT assay
Cytotoxicity against human Huh7.5 cells after 96 hrs by MTT assay
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[PMID: 22196120] |
| Huh-7 | EC50 |
33.19 μM
Compound: 22
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Antiviral activity against Hepatitis C virus infected in human Huh7.5 cells assessed as inhibition of viral replication after 96 hrs by quantitative RT-PCR analysis
Antiviral activity against Hepatitis C virus infected in human Huh7.5 cells assessed as inhibition of viral replication after 96 hrs by quantitative RT-PCR analysis
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[PMID: 22196120] |
| KB | ED50 |
>10 μg/mL
Compound: 2
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Cytotoxicity against human KB cells by tetrazolium salt-based colorimetric assay
Cytotoxicity against human KB cells by tetrazolium salt-based colorimetric assay
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[PMID: 1431932] |
| RD | IC50 |
10 μg/mL
Compound: Geraniin
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Antiviral activity against C4 type Human enterovirus 71 FY0805 infected in human RD cells assessed as inhibition of viral replication by plaque reduction assay
Antiviral activity against C4 type Human enterovirus 71 FY0805 infected in human RD cells assessed as inhibition of viral replication by plaque reduction assay
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[PMID: 22342145] |
| TE-671 | ED50 |
>10 μg/mL
Compound: 2
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Cytotoxicity against human TE671 cells by tetrazolium salt-based colorimetric assay
Cytotoxicity against human TE671 cells by tetrazolium salt-based colorimetric assay
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[PMID: 1431932] |
The IC50 value of TNF-α release inhibition is 43 μM for Geraniin[1]. Geraniin has long been used as a medicinal herb and possesses numerous activities including anticancer, anti-inflammatory, and anti-hyperglycemic activities. Geraniin significantly decreases the viability of OVCAR3 and SKOV3 cells in a concentration-dependent fashion. The IC50 value for Geraniin treatment is 34.5±2.8 μM in OVCAR3 cells and 23.6±1.9 μM in SKOV3 cells. However, Geraniin up to the maximal concentration used (80 μM) has no significant impact on the viability of normal human ovarian surface epithelial cells. Treatment with 10 and 40 μM of Geraniin for 48 h causes a significant increase in apoptosis (16.8±1.2% and 22.6±1.4%, respectively), compared with control OVCAR3 cells (3.9±1.1%). Similar results are observed in SKOV3 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 60976-49-0
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Appearance Solid
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Peso molecular 952.64
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Fòrmula C41H28O27
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Color Off-white to yellow
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SMILES
O=C(OC[C@H](O[C@@H](OC(C1=CC(O)=C(O)C(O)=C1)=O)[C@H]2OC3=O)[C@H](OC(C(C4C5=C3C=C(O)C(O)=C5OC6(O)C4(O)O)=CC6=O)=O)[C@H]2O7)C8=CC(O)=C(O)C(O)=C8C9=C(O)C(O)=C(O)C=C9C7=O
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (3)
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Journal Impact Factor
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Most Recent
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J Transl Med
Delineation of colorectal cancer ligand-receptor interactions and their roles in the tumor microenvironment and prognosis. [Abstract]2021 Dec 7;19(1):497. PMID: 34876143 -
J Cell Mol Med
Geraniin Alleviates Mouse Laser-Induced Choroidal Neovascularisation by Inhibiting Choroidal Endothelial Cell ACE2/Ang-(1-7)/MasR/IL-10 Pathway. [Abstract]2024 Dec;28(23):e70228. PMID: 39622780 -
J Chromatogr A
Screening and identification of acetylcholinesterase inhibitors from Terminalia chebula fruits by immobilized enzyme on cellulose filter paper coupled with ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry and molecular docking. [Abstract]2022 Jan 25:1663:462784. PMID: 34974370
Solvente y solubilidad
DMSO : 100 mg/mL (104.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.62 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.62 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocolo
Human ovarian cancer cell lines OVCAR3 and SKOV3 are used. Cells are exposed to different concentrations (5, 10, 20, 40, and 80 μM) of Geraniin for 48 h and examined for viability, apoptosis, and gene expression. The concentration range is selected based on previous studies[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureza y Documentación
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Ficha de datos (282 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Okabe S, et al. New TNF-alpha releasing inhibitors, geraniin and corilagin, in leaves of Acer nikoense, Megusurino-ki. Biol Pharm Bull. 2001 Oct;24(10):1145-8. [Content Brief]
[2]. Wang X, et al. Geraniin suppresses ovarian cancer growth through inhibition of NF-κB activation and downregulation of Mcl-1 expression. J Biochem Mol Toxicol. 2017 Sep;31(9). [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.0497 mL | 5.2486 mL | 10.4971 mL | 26.2429 mL |
| 5 mM | 0.2099 mL | 1.0497 mL | 2.0994 mL | 5.2486 mL | |
| 10 mM | 0.1050 mL | 0.5249 mL | 1.0497 mL | 2.6243 mL | |
| 15 mM | 0.0700 mL | 0.3499 mL | 0.6998 mL | 1.7495 mL | |
| 20 mM | 0.0525 mL | 0.2624 mL | 0.5249 mL | 1.3121 mL | |
| 25 mM | 0.0420 mL | 0.2099 mL | 0.4199 mL | 1.0497 mL | |
| 30 mM | 0.0350 mL | 0.1750 mL | 0.3499 mL | 0.8748 mL | |
| 40 mM | 0.0262 mL | 0.1312 mL | 0.2624 mL | 0.6561 mL | |
| 50 mM | 0.0210 mL | 0.1050 mL | 0.2099 mL | 0.5249 mL | |
| 60 mM | 0.0175 mL | 0.0875 mL | 0.1750 mL | 0.4374 mL | |
| 80 mM | 0.0131 mL | 0.0656 mL | 0.1312 mL | 0.3280 mL | |
| 100 mM | 0.0105 mL | 0.0525 mL | 0.1050 mL | 0.2624 mL |
Geraniin Related Classifications
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