L-Cysteine
Based on 13 publication(s) in Google Scholar
L-Cysteine (Cysteine) is an orally active conditionally essential amino acid with hypoglycemic effects, which acts as a precursor for biologically active molecules such as hydrogen sulphide (H2S), glutathione and taurine. L-Cysteine promotes the proliferation and differentiation of neural stem cells via the CBS/H2S pathway. L-Cysteine suppresses ghrelin and reduces appetite in rodents and humans. L-Cysteine can be used as an anorectic agent.
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- Pureza : 99.95%
- No. CAS: 52-90-4
- Fòrmula: C3H7NO2S
- Peso molecular:121.16
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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L-GlutamineEndogenous Metabolite -
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L-LeucinemTOR activator
Publications Citing Use of MedChemExpress (MCE) L-Cysteine
More- Nature. 2025 Jul;643(8070):192-200. [Abstract]
- Cell Metab. 2025 Jan 7;37(1):291-304.e9. [Abstract]
- J Nanobiotechnology. 2026 Feb 28;24(1):315. [Abstract]
- Adv Sci (Weinh). 2025 Nov 21:e10500. [Abstract]
- Int J Biol Sci. 2023 Aug 21;19(14):4457-4475. [Abstract]
- Life Metab. 2025 Oct 8.
- Probiotics Antimicrob Proteins. 2025 Nov 26. [Abstract]
- J Chromatogr A. 2024 Apr 12:1720:464776. [Abstract]
- J Integr Neurosci. 2026 Jul 29;25(7):50683.
- Amino Acids. 2024 Sep 18;56(1):56. [Abstract]
- Sci Total Environ. 2024 Dec 20:957:177678. [Abstract]
- Research Square Preprint. 2024 Jan 24.
- SSRN. 2023 Nov 17.
Ver todos los productos específicos de isoformas Endogenous Metabolite
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Actividad biológica
Descripciòn
IC50 & Target
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Microbial Metabolite |
Human Endogenous Metabolite |
ERK1 |
ERK2 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | EC50 |
161 μM
Compound: 5862
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Substrate uptake by the Excitatory Amino Acid Transporter 3 (EAAT3, SLC1A1) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC1A1 cells
Substrate uptake by the Excitatory Amino Acid Transporter 3 (EAAT3, SLC1A1) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC1A1 cells
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10.5281/zenodo.7361326 |
| HEK293 | EC50 |
161 μM
Compound: 5862
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Substrate uptake by the Excitatory Amino Acid Transporter 3 (EAAT3, SLC1A1) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC1A1 cells (PubChem AID: 1794812)
Substrate uptake by the Excitatory Amino Acid Transporter 3 (EAAT3, SLC1A1) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC1A1 cells (PubChem AID: 1794812)
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10.5281/zenodo.7361326 |
| HEK293 | EC50 |
376 μM
Compound: 5862
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Substrate uptake by the Excitatory Amino Acid Transporter 1 (EAAT1, SLC1A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC1A3 cells
Substrate uptake by the Excitatory Amino Acid Transporter 1 (EAAT1, SLC1A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC1A3 cells
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10.5281/zenodo.6782621 |
| HEK293 | EC50 |
376 μM
Compound: 5862
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Substrate uptake by the Excitatory Amino Acid Transporter 1 (EAAT1, SLC1A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC1A3 cells (PubChem AID: 1745864)
Substrate uptake by the Excitatory Amino Acid Transporter 1 (EAAT1, SLC1A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC1A3 cells (PubChem AID: 1745864)
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10.5281/zenodo.6782621 |
| HeLa | IC50 |
>50 μM
Compound: 3a
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Cytotoxic activity against HeLa cells
Cytotoxic activity against HeLa cells
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[PMID: 17524640] |
| SNB-19 | IC50 |
116 μM
Compound: L-cystine
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Inhibition of amino acid transport system xc- in human SNB19 cells assessed as [3H]L-glutamate uptake by liquid scintillation counting
Inhibition of amino acid transport system xc- in human SNB19 cells assessed as [3H]L-glutamate uptake by liquid scintillation counting
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[PMID: 19932968] |
In Vitro
L-Cysteine (0.1-50 μM, 24 and 48 h) increases cell viability of neural stem cells (NSCs) and promotes proliferation and neuronal differentiation of NSCs[2].
L-cysteine (1 μM, 0-90 min) activates ERK1/2 via CBS/H2S pathway in NSCs[2].
L-Cysteine (0.1-20 mM, 30 min) results in a decrease in glucose uptake in soleus muscle[3].
L-Cysteine (0.5 mM, 30 min) increases GLUT3 levels in SH-SY5Y cells and GLUT4 levels in SH-SY5Y cells, soleus muscle and C2C12 muscle cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:NSCs
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Concentration:0.1-50 μM
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Incubation Time:24 and 48 h
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Result:Obviously improved the cell viability of NSCs in a dose-dependent manner at lower doses (0.5, 1, 5 μM) and increased the ratio of BrdU-positive cells against total NSCs at 24 h.
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Cell Line:NSCs
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Concentration:1 μM
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Incubation Time:0-90 min
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Result:Induced overexpression of phosphorylated ERK (p-ERK1/2) in NSCs.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Wistar rats following an overnight 16-h fast[1]
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Dosage:1-4 mmol/kg
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Administration:p.o. or i.p., a single dose for 1 h
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Result:Dose-dependently decreased food intake in rats and mice and significantly increased cFLI in the area postrema in rats.
Chemical Information
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No. CAS 52-90-4
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Appearance Solid
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Peso molecular 121.16
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Fòrmula C3H7NO2S
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Color White to off-white
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SMILES
N[C@@H](CS)C(O)=O
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Synonyms
Cysteine
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (13)
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Journal Impact Factor
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Most Recent
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Nature
2025 Jul;643(8070):192-200. PMID: 39695227 -
Cell Metab
Lighting up arginine metabolism reveals its functional diversity in physiology and pathology. [Abstract]2025 Jan 7;37(1):291-304.e9. PMID: 39413790 -
J Nanobiotechnology
Targeted inhibition of RGS19 alleviates renal fibrosis by restoring autophagy and modulating immune cell infiltration. [Abstract]2026 Feb 28;24(1):315. PMID: 41761314 -
Adv Sci (Weinh)
Targeting the SOX9/TIMP1 Axis with iRGD-Conjugated Nanoplatform Enhances Dendritic Cell Function and Photodynamic Immunotherapy in Gastric Cancer. [Abstract]2025 Nov 21:e10500. PMID: 41270217 -
Int J Biol Sci
MKL-1 suppresses ferroptosis by activating system Xc- and increasing glutathione synthesis. [Abstract]2023 Aug 21;19(14):4457-4475. PMID: 37781038 -
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Probiotics Antimicrob Proteins
Potential Benefits of Metformin Via Roseburia Intestinalis in High-fat Diet-fed Mice With Colorectal Adenomas. [Abstract]2025 Nov 26. PMID: 41296177 -
J Chromatogr A
Preparation of thiol-free amino acid plasma matrix for quantification of thiol amino acids and their oxidized forms in human plasma by UPLC-MS/MS. [Abstract]2024 Apr 12:1720:464776. PMID: 38432109 -
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Amino Acids
Identification of amino acids metabolomic profiling in human plasma distinguishes lupus nephritis from systemic lupus erythematosus. [Abstract]2024 Sep 18;56(1):56. PMID: 39292313 -
Sci Total Environ
Network pharmacology and transcriptomics reveal androgen receptor as a potential protein target for 6PPD-quinone. [Abstract]2024 Dec 20:957:177678. PMID: 39581451 -
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Solvente y solubilidad
In Vitro:
H2O : 5 mg/mL (41.27 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (825.35 mM); Clear solution; Need ultrasonic
Protocolo
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureza y Documentación
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Ficha de datos (277 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. McGavigan AK, et al. L-cysteine suppresses ghrelin and reduces appetite in rodents and humans. Int J Obes (Lond). 2015 Mar;39(3):447-55. [Content Brief]
[2]. Wang Z, et al. L-Cysteine promotes the proliferation and differentiation of neural stem cells via the CBS/H₂S pathway. Neuroscience. 2013 May 1;237:106-17. [Content Brief]
[3]. Gazit V, et al. L-cysteine increases glucose uptake in mouse soleus muscle and SH-SY5Y cells. Metab Brain Dis. 2003 Sep;18(3):221-31. [Content Brief]
[4]. Takemoto Y. The central effect of L-cysteine on cardiovascular system of the conscious rat. Jpn J Physiol. 1995;45(5):771-83. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 8.2535 mL | 41.2677 mL | 82.5355 mL | 206.3387 mL |
| 5 mM | 1.6507 mL | 8.2535 mL | 16.5071 mL | 41.2677 mL | |
| 10 mM | 0.8254 mL | 4.1268 mL | 8.2535 mL | 20.6339 mL | |
| 15 mM | 0.5502 mL | 2.7512 mL | 5.5024 mL | 13.7559 mL | |
| 20 mM | 0.4127 mL | 2.0634 mL | 4.1268 mL | 10.3169 mL | |
| 25 mM | 0.3301 mL | 1.6507 mL | 3.3014 mL | 8.2535 mL | |
| 30 mM | 0.2751 mL | 1.3756 mL | 2.7512 mL | 6.8780 mL | |
| 40 mM | 0.2063 mL | 1.0317 mL | 2.0634 mL | 5.1585 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
L-Cysteine Related Classifications
HY-Y0337 Related Classifications
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