MCP110
Based on 2 publication(s) in Google Scholar
MCP110 is an inhibitor of Ras/Raf-1 interaction. MCP110 blocks the interaction of Ras with Raf. MCP110 disrupts this interaction might can be used for the research of human tumors.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.82%
- No. CAS: 521310-51-0
- Fòrmula: C33H36N2O3
- Peso molecular:508.65
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Almacenamiento:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) MCP110
More
Actividad biológica
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Ras |
RAF |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | GI50 |
16.2 μM
Compound: 5
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Antiproliferative activity was evaluated using HCT116 cells in WST-1 assay
Antiproliferative activity was evaluated using HCT116 cells in WST-1 assay
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[PMID: 15225706] |
| HCT-116 | GI50 |
20.6 μM
Compound: 5
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Ability of compound to inhibit anchorage independent colony formation (soft agar growth assay) in HCT116 cells
Ability of compound to inhibit anchorage independent colony formation (soft agar growth assay) in HCT116 cells
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[PMID: 15225706] |
| HeLa | IC50 |
9 μM
Compound: 5
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Inhibition of EGF-stimulated Elk1-luciferase reporter assay in HeLa cells
Inhibition of EGF-stimulated Elk1-luciferase reporter assay in HeLa cells
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[PMID: 15225706] |
MCP110 (10 and 20 uM) inhibits H-Ras (V12)-induced AP-1 activation in HEK293 cells. Ras stimulation of Raf-1 activity is significantly decreased by MCP110 (20 μM) [1].
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MCP110 (1, 2, 5, 10, and 20 μM) shows a dose-dependent inhibition of elevated Raf-1 activity in fibrosarcoma HT1080 cells[1].
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MCP110 (20 μM) decreases cyclin D level stimulated by the treatment with EGF (100 ng/mL), platelet-derived growth factor (20 ng/mL) , serum, or phorbol 12-myristate 13-acetate (100 nM) in A549 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 521310-51-0
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Appearance Oil
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Peso molecular 508.65
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Fòrmula C33H36N2O3
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Color Colorless to light yellow
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SMILES
O=C(N(CC1=CC=C(OCC2=CC=CC=C2)C(OC)=C1)CCC3=NC=CC=C3)CCCCC4=CC=CC=C4
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
Osteoblast-Derived ECM1 Promotes Anti-Androgen Resistance in Bone Metastatic Prostate Cancer. [Abstract]2025 Jan;12(2):e2407662. PMID: 39563492 -
Gene
ALDH6A1 weakens the progression of colon cancer via modulating the RAS/RAF/MEK/ERK pathway in cancer cell lines. [Abstract]2022 Oct 30:842:146757. PMID: 35907565
Solvente y solubilidad
DMSO : 250 mg/mL (491.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.09 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9660 mL | 9.8299 mL | 19.6599 mL | 49.1497 mL |
| 5 mM | 0.3932 mL | 1.9660 mL | 3.9320 mL | 9.8299 mL | |
| 10 mM | 0.1966 mL | 0.9830 mL | 1.9660 mL | 4.9150 mL | |
| 15 mM | 0.1311 mL | 0.6553 mL | 1.3107 mL | 3.2766 mL | |
| 20 mM | 0.0983 mL | 0.4915 mL | 0.9830 mL | 2.4575 mL | |
| 25 mM | 0.0786 mL | 0.3932 mL | 0.7864 mL | 1.9660 mL | |
| 30 mM | 0.0655 mL | 0.3277 mL | 0.6553 mL | 1.6383 mL | |
| 40 mM | 0.0491 mL | 0.2457 mL | 0.4915 mL | 1.2287 mL | |
| 50 mM | 0.0393 mL | 0.1966 mL | 0.3932 mL | 0.9830 mL | |
| 60 mM | 0.0328 mL | 0.1638 mL | 0.3277 mL | 0.8192 mL | |
| 80 mM | 0.0246 mL | 0.1229 mL | 0.2457 mL | 0.6144 mL | |
| 100 mM | 0.0197 mL | 0.0983 mL | 0.1966 mL | 0.4915 mL |
MCP110 Related Classifications
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