PMSF
Based on 161 publication(s) in Google Scholar
PMSF (Phenylmethylsulfonyl fluoride) is a nonspecific and irreversible serine proteases inhibitor. PMSF inhibits some thiol proteases, non-protease enzymes, and acetylcholinesterase. PMSF can be used to prepare protein extracts from tissue and cell lysates.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.32%
- No. CAS: 329-98-6
- Fòrmula: C7H7FO2S
- Peso molecular:174.19
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Publications Citing Use of MedChemExpress (MCE) PMSF
More- Signal Transduct Target Ther. 2025 Jun 30;10(1):205. [Abstract]
- Cancer Cell. 2025 Jul 17:S1535-6108(25)00273-9. [Abstract]
- Cell. 2026 Jun 11;189(12):3553-3570.e30. [Abstract]
- Cell. 2026 Feb 5;189(3):853-871.e31. [Abstract]
- Mol Cancer. 2026 May 13.
- Circulation. 2022 Mar;145(9):675-687. [Abstract]
- Mol Plant. 2025 Nov 17:S1674-2052(25)00394-6. [Abstract]
- Nat Metab. 2026 Jan;8(1):74-95. [Abstract]
- Nat Biomed Eng. 2025 Jun;9(6):952-966. [Abstract]
- Bioact Mater. 2025 Oct 29:56:468-482. [Abstract]
- Cancer Res. 2025 Feb 17;85(4):692-703. [Abstract]
- J Extracell Vesicles. 2025 Sep;14(9):e70162. [Abstract]
- J Extracell Vesicles. 2025 Jan;14(1):e70041. [Abstract]
- Bone Res. 2025 Mar 3;13(1):30. [Abstract]
- Autophagy. 2025 Jul 11:1-19. [Abstract]
- Autophagy. 2024 Nov;20(11):2556-2570. [Abstract]
- Autophagy. 2024 Oct;20(10):2221-2237. [Abstract]
- Autophagy. 2023 Jun;19(6):1745-1763. [Abstract]
- Autophagy. 2021 Oct;17(10):3030-3047. [Abstract]
- Nat Commun. 2026 Jun 15. [Abstract]
- Nat Commun. 2024 Dec 4;15(1):10543. [Abstract]
- J Adv Res. 2026 Mar 23:S2090-1232(26)00271-7. [Abstract]
- J Adv Res. 2024 Feb:56:125-136. [Abstract]
- Redox Biol. 2026 Jul:94:104146. [Abstract]
- Adv Sci (Weinh). 2026 May 26:e15158. [Abstract]
- Adv Sci (Weinh). 2026 Mar 12:e20455. [Abstract]
- MedComm (2020). 2025 Oct 30;6(11):e70438. [Abstract]
- Adv Sci (Weinh). 2025 Jul;12(25):e2501046. [Abstract]
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- Braz J Biol. 2023 Jan 6:82:e266573. [Abstract]
- Research Square Print. December 5th, 2022.
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- Korean Journal of Medicinal Crop Science. 2021; 29(2):135.
- Heliyon. 2020 Aug;6(8):e04793. [Abstract]
Ver todos los productos específicos de isoformas Cathepsin
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Actividad biológica
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Neutrophil | IC50 |
130.93 μM
Compound: PMSF
|
Antiinflammatory activity in neutrophil assessed as inhibition of fMLP induced elastase release
Antiinflammatory activity in neutrophil assessed as inhibition of fMLP induced elastase release
|
[PMID: 17197180] |
| Neutrophil | IC50 |
35.68 μM
Compound: phenylmethylsulfonyl fluoride
|
Antiinflammatory activity in human neutrophils assessed as inhibition of fMet-Leu-Phe/Cytochalasin B-induced elastase release at 10 ug/mL
Antiinflammatory activity in human neutrophils assessed as inhibition of fMet-Leu-Phe/Cytochalasin B-induced elastase release at 10 ug/mL
|
[PMID: 18163582] |
| Neutrophil | IC50 |
204.08 μM
Compound: phenylmethylsulfonyl fluoride
|
Antiinflammatory activity in human neutrophils assessed as inhibition of formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B-induced elastase release
Antiinflammatory activity in human neutrophils assessed as inhibition of formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B-induced elastase release
|
[PMID: 18211005] |
| Neutrophil | IC50 |
202.3 μM
Compound: phenylmethylsulfonyl fluoride
|
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/Cytochalasin B-induced elastase release
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/Cytochalasin B-induced elastase release
|
[PMID: 18817445] |
| Neutrophil | IC50 |
130.9 μM
Compound: PMSF
|
Antiinflammatory activity in fMLP/cytochalasin B-stimulated human neutrophils assessed as inhibition of elastase release after 5 mins
Antiinflammatory activity in fMLP/cytochalasin B-stimulated human neutrophils assessed as inhibition of elastase release after 5 mins
|
[PMID: 19110343] |
| Neutrophil | IC50 |
35.24 μg/mL
Compound: Phenylmethylsulfonyl fluoride
|
Antiinflammatory activity against human neutrophils assessed as inhibition of fMLP/CB-induced elastase release
Antiinflammatory activity against human neutrophils assessed as inhibition of fMLP/CB-induced elastase release
|
[PMID: 19128011] |
| Neutrophil | IC50 |
203.5 μM
Compound: phenylmethylsulfonyl fluoride
|
Antiinflammatory activity in human neutrophils assessed as inhibition of fMet-Leu-Phe/Cytochalasin B-induced elastase release treated 5 mins before fMet-Leu-Phe/Cytochalasin B challenge
Antiinflammatory activity in human neutrophils assessed as inhibition of fMet-Leu-Phe/Cytochalasin B-induced elastase release treated 5 mins before fMet-Leu-Phe/Cytochalasin B challenge
|
[PMID: 19203247] |
| Neutrophil | IC50 |
95 μM
Compound: PMSF
|
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/Cytochalasin B-induced elastase release
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/Cytochalasin B-induced elastase release
|
[PMID: 19583252] |
| Neutrophil | IC50 |
130.9 μM
Compound: PMSF
|
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/cytochalasin B-induced elastase release
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/cytochalasin B-induced elastase release
|
[PMID: 20036537] |
| Neutrophil | IC50 |
19.89 μg/mL
Compound: PMSF
|
Antiinflammatory activity in human neutrophils assessed as inhibition of FMLP/CB-induced elastase release
Antiinflammatory activity in human neutrophils assessed as inhibition of FMLP/CB-induced elastase release
|
[PMID: 21106454] |
| Neutrophil | IC50 |
95 μM
Compound: PMSF
|
Inhibition of fMLP/CB-activated human neutrophil degranulation assessed as inhibition of elastase release using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as a substrate after 5 mins
Inhibition of fMLP/CB-activated human neutrophil degranulation assessed as inhibition of elastase release using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as a substrate after 5 mins
|
[PMID: 21417387] |
| Neutrophil | IC50 |
95 μM
Compound: PMSF
|
Inhibition of FMLP/CB-stimulated elastase release in human neutrophils treated for 5 mins before FMLP/CB challenge using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as a substrate
Inhibition of FMLP/CB-stimulated elastase release in human neutrophils treated for 5 mins before FMLP/CB challenge using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as a substrate
|
[PMID: 21441032] |
| Neutrophil | IC50 |
22.8 μg/mL
Compound: PMSF
|
Antiinflammatory activity in human neutrophils assessed as inhibition of FMLP/CB-induced elastase release incubated for 5 mins prior to FMLP/CB-challenge by spectrophotometry
Antiinflammatory activity in human neutrophils assessed as inhibition of FMLP/CB-induced elastase release incubated for 5 mins prior to FMLP/CB-challenge by spectrophotometry
|
[PMID: 22413887] |
| Neutrophil | IC50 |
131 μg/mL
Compound: PMSF
|
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release using MeO-Suc-Ala-Ala-Pro-Val-MC as substrate by spectrophotometry
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release using MeO-Suc-Ala-Ala-Pro-Val-MC as substrate by spectrophotometry
|
[PMID: 22595179] |
| Neutrophil | IC50 |
95 μM
Compound: Phenylmethylsulfonyl fluoride
|
Inhibition of fMLF/CB-induced human Neutrophil activation assessed as reduction in superoxide anion
Inhibition of fMLF/CB-induced human Neutrophil activation assessed as reduction in superoxide anion
|
[PMID: 37683361] |
PMSF (2 mM) inhibits carbachol-stimulated inositol phosphate accumulation in the presence of Li+ by only 15%-19%. PMSF inhibition of phosphoinositide turnover is due to one or more steps following phosphoinositide breakdown[1]. PMSF inhibits the acylation of the inositol residue of GPI intermediates in bloodstream-form T. brucei. PMSF inhibits the formation of glycolipid C but does not inhibit fatty acid remodeling in vitro. PMSF inhibits GPI acylation and ethanolamine phosphate addition in procyclic trypanosomes but not in Hela cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
No. CAS 329-98-6
-
Appearance Solid
-
Peso molecular 174.19
-
Fòrmula C7H7FO2S
-
Color White to off-white
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SMILES
O=S(CC1=CC=CC=C1)(F)=O
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Synonyms
Phenylmethylsulfonyl fluoride; Benzylsulfonyl fluoride
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Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (161)
-
Journal Impact Factor
-
Most Recent
-
Signal Transduct Target Ther
Serine/threonine/tyrosine kinase 1 drives pancreatic carcinogenesis via GSK3β sequestration-mediated Wnt/β-catenin pathway hyperactivation. [Abstract]2025 Jun 30;10(1):205. PMID: 40588478 -
Cancer Cell
2025 Jul 17:S1535-6108(25)00273-9. PMID: 40712567 -
Cell
2026 Jun 11;189(12):3553-3570.e30. PMID: 42049018 -
Cell
Peripheral cancer attenuates amyloid pathology in Alzheimer's disease via cystatin-c activation of TREM2. [Abstract]2026 Feb 5;189(3):853-871.e31. PMID: 41576952 -
-
Circulation
Ablation of Plasma Prekallikrein Decreases Low-Density Lipoprotein Cholesterol by Stabilizing Low-Density Lipoprotein Receptor and Protects Against Atherosclerosis. [Abstract]2022 Mar;145(9):675-687. PMID: 35189703 -
Mol Plant
Pathogen-induced H2O2 triggers phosphorylation of the RNA-binding protein RRM2R to suppress OsMAPKKK18 intron retention for orchestrating rice immunity and growth. [Abstract]2025 Nov 17:S1674-2052(25)00394-6. PMID: 41254943 -
Nat Metab
2026 Jan;8(1):74-95. PMID: 41507665 -
Nat Biomed Eng
Concurrent intratumoural Treg cell depletion and CD8+ T cell expansion via a cleavable anti-4-1BB-interleukin-15 fusion protein. [Abstract]2025 Jun;9(6):952-966. PMID: 39623095 -
Bioact Mater
Study on the mechanism of hUCMSCs-derived 3D exosome combined with HAMA microneedles in the treatment of deep second-degree burns. [Abstract]2025 Oct 29:56:468-482. PMID: 41234297 -
Cancer Res
Adenosine Uptake through the Nucleoside Transporter ENT1 Suppresses Antitumor Immunity and T-cell Pyrimidine Synthesis. [Abstract]2025 Feb 17;85(4):692-703. PMID: 39652568 -
J Extracell Vesicles
Targeted Blockage of Pathological Extracellular Vesicles and Particles From Fibroblast-Like Synoviocytes for Osteoarthritis Relief: Proteomic Analysis and Cellular Effect. [Abstract]2025 Sep;14(9):e70162. PMID: 40919882 -
J Extracell Vesicles
Extracellular Vesicles From Bone Marrow-Derived Macrophages Enriched in ARG1 Enhance Microglial Phagocytosis and Haematoma Clearance Following Intracerebral Haemorrhage. [Abstract]2025 Jan;14(1):e70041. PMID: 39868438 -
Bone Res
Enhanced SIRT3 expression restores mitochondrial quality control mechanism to reverse osteogenic impairment in type 2 diabetes mellitus. [Abstract]2025 Mar 3;13(1):30. PMID: 40025004 -
Autophagy
2025 Jul 11:1-19. PMID: 40568837 -
Autophagy
Signal-sustained imaging of mitophagy with an Enzyme-Activatable Metabolic Lipid-Labeling Probe. [Abstract]2024 Nov;20(11):2556-2570. PMID: 38873937 -
Autophagy
PLK2-mediated phosphorylation of SQSTM1 S349 promotes aggregation of polyubiquitinated proteins upon proteasomal dysfunction. [Abstract]2024 Oct;20(10):2221-2237. PMID: 39316746 -
Autophagy
The Valsa mali effector Vm1G-1794 protects the aggregated MdEF-Tu from autophagic degradation to promote infection in apple. [Abstract]2023 Jun;19(6):1745-1763. PMID: 36449354 -
Autophagy
RNF186 regulates EFNB1 (ephrin B1)-EPHB2-induced autophagy in the colonic epithelial cells for the maintenance of intestinal homeostasis. [Abstract]2021 Oct;17(10):3030-3047. PMID: 33280498 -
Nat Commun
2026 Jun 15. PMID: 42297841 -
Nat Commun
Epstein-Barr virus infection upregulates extracellular OLFM4 to activate YAP signaling during gastric cancer progression. [Abstract]2024 Dec 4;15(1):10543. PMID: 39627192 -
J Adv Res
MyD88 inhibitory Peptide-2 (MIP2) improves neurological outcomes and reduces neuroinflammation after intracerebral Hemorrhage-mediated secondary brain injury. [Abstract]2026 Mar 23:S2090-1232(26)00271-7. PMID: 41881250 -
J Adv Res
Matrix metalloproteinase-9 inhibition prevents aquaporin-4 depolarization-mediated glymphatic dysfunction in Parkinson's disease. [Abstract]2024 Feb:56:125-136. PMID: 36940850 -
Redox Biol
SERPINE1 drives ferroptosis in acute respiratory distress syndrome by disrupting mitochondrial NAD+ homeostasis and suppressing Sirt3 activity. [Abstract]2026 Jul:94:104146. PMID: 42190562 -
Adv Sci (Weinh)
Genome-Wide CRISPR Screen Identifies a microRNA Orchestrating Pleiotropic Resistance to Targeted Therapy and T Cell Immunity in Melanoma. [Abstract]2026 May 26:e15158. PMID: 42189126 -
Adv Sci (Weinh)
IGFBP5 Restores Endometrial Receptivity and Rescues Implantation Failure in Polycystic Ovary Syndrome. [Abstract]2026 Mar 12:e20455. PMID: 41816941 -
MedComm (2020)
Implant Renal Injury-Responsive Cells to Supplement Erythropoietin and Protect Kidney Injury. [Abstract]2025 Oct 30;6(11):e70438. PMID: 41179707 -
Adv Sci (Weinh)
Mechanical Load-Induced Upregulation of Talin2 through Non-Canonical Deubiquitination of OTUB1 Drives Facet Joint Osteoarthritis Pathogenesis. [Abstract]2025 Jul;12(25):e2501046. PMID: 40279639 -
Adv Sci (Weinh)
Targeting Antigen-Presenting Cells to Enhance the Tumor-Spleen Immunity Cycle through Liposome-Neoantigen Vaccine. [Abstract]2025 May;12(19):e2500021. PMID: 40125791 -
Sci Adv
Multidimensional mapping of stimulation-responsive regulatory elements and candidate causal variants in T cell activation. [Abstract]2026 Jan 2;12(1):eady2539. PMID: 41477821 -
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Cell Death Dis
Identification of KW-2449 as a dual inhibitor of ferroptosis and necroptosis reveals that autophagy is a targetable pathway for necroptosis inhibitors to prevent ferroptosis. [Abstract]2024 Oct 21;15(10):764. PMID: 39433736 -
Cell Death Dis
Sensing of endogenous retroviruses-derived RNA by ZBP1 triggers PANoptosis in DNA damage and contributes to toxic side effects of chemotherapy. [Abstract]2024 Oct 27;15(10):779. PMID: 39465258 -
Cell Death Dis
4EBP1 senses extracellular glucose deprivation and initiates cell death signaling in lung cancer. [Abstract]2022 Dec 27;13(12):1075. PMID: 36575176 -
Small
Photo-Enhanced CRISPR/Cas9 System Enables Robust PD-L1 Gene Disruption in Cancer Cells and Cancer Stem-Like Cells for Efficient Cancer Immunotherapy. [Abstract]2020 Dec 2:e2004879. PMID: 33289336 -
Cell Commun Signal
Retinoic acid enhances γδ T cell cytotoxicity in nasopharyngeal carcinoma by reversing immune exhaustion. [Abstract]2025 Mar 29;23(1):156. PMID: 40158172 -
Cell Commun Signal
L-Fucose promotes enteric nervous system regeneration in type 1 diabetic mice by inhibiting SMAD2 signaling pathway in enteric neural precursor cells. [Abstract]2023 Oct 5;21(1):273. PMID: 37798789 -
Phytomedicine
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Cell Death Discov
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Heliyon
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Solvente y solubilidad
DMSO : 250 mg/mL (1435.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Isopropanol : 60 mg/mL (344.45 mM; Need ultrasonic)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2 mg/mL (11.48 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2 mg/mL (11.48 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: Corn Oil
Solubility: 20 mg/mL (114.82 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocolo
Male ICR mice weighing 18 to 25 g are used in the assay. PMSF is dissolved in sesame oil and administered i.p. at a volume of 0.1 mL/10 g b.wt. PMSF is always administered 10 min before i.v. anandamide or vehicle injections. Mice are acclimated to the evaluation room overnight without interruption of food or water. After i.v. anandamide or vehicle administration each animal is evaluated as follows: tail-flick latency (antinociception) response at 5 min and spontaneous (locomotor) activity at 5 to 15 min; or core (rectal) temperature at 5 min and ring-immobility (catalepsy) at 5 to 10 min.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureza y Documentación
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Ficha de datos (280 KB)
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SDS (642 KB)
- English - EN (642 KB)
- Français - FR (642 KB)
- Deutsch - DE (642 KB)
- Norwegian - NO (642 KB)
- Español - ES (642 KB)
- Swedish - SV (642 KB)
- Italian - IT (642 KB)
- Korean - KR (642 KB)
- Portuguese - PT (642 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Sekar, M.C. and B.D. Roufogalis, Differential effects of phenylmethanesulfonyl fluoride (PMSF) on carbachol and potassium stimulated phosphoinositide turnover and contraction in longitudinal smooth muscle of guinea pig ileum. Cell Calcium, 1984. 5(3): p. [Content Brief]
[2]. Guther, M.L., W.J. Masterson, and M.A. Ferguson, The effects of phenylmethylsulfonyl fluoride on inositol-acylation and fatty acid remodeling in African trypanosomes. J Biol Chem, 1994. 269(28): p. 18694-701. [Content Brief]
[3]. Compton, D.R. and B.R. Martin, The effect of the enzyme inhibitor phenylmethylsulfonyl fluoride on the pharmacological effect of anandamide in the mouse model of cannabimimetic activity. J Pharmacol Exp Ther, 1997. 283(3): p. 1138-43. [Content Brief]
[4]. Asadi Akbarabadi E, et a. PMSF Attenuates Morphine Antinociceptive Tolerance and Dependence in Mice: Its Association with the Oxidative Stress Suppression. Iran J Pharm Res. 2021 Summer;20(3):300-309. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Isopropanol / DMSO | 1 mM | 5.7409 mL | 28.7043 mL | 57.4086 mL | 143.5214 mL |
| 5 mM | 1.1482 mL | 5.7409 mL | 11.4817 mL | 28.7043 mL | |
| 10 mM | 0.5741 mL | 2.8704 mL | 5.7409 mL | 14.3521 mL | |
| 15 mM | 0.3827 mL | 1.9136 mL | 3.8272 mL | 9.5681 mL | |
| 20 mM | 0.2870 mL | 1.4352 mL | 2.8704 mL | 7.1761 mL | |
| 25 mM | 0.2296 mL | 1.1482 mL | 2.2963 mL | 5.7409 mL | |
| 30 mM | 0.1914 mL | 0.9568 mL | 1.9136 mL | 4.7840 mL | |
| 40 mM | 0.1435 mL | 0.7176 mL | 1.4352 mL | 3.5880 mL | |
| 50 mM | 0.1148 mL | 0.5741 mL | 1.1482 mL | 2.8704 mL | |
| 60 mM | 0.0957 mL | 0.4784 mL | 0.9568 mL | 2.3920 mL | |
| 80 mM | 0.0718 mL | 0.3588 mL | 0.7176 mL | 1.7940 mL | |
| 100 mM | 0.0574 mL | 0.2870 mL | 0.5741 mL | 1.4352 mL |
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