2. Cell Cycle/DNA Damage Epigenetics Apoptosis
  3. HDAC Apoptosis
  4. HDAC1/3-IN-1

HDAC1/3-IN-1 is a selective HDAC1/3 inhibitor, with IC50 values of 256 nM and 340.3 nM against HDAC1 and HDAC3, respectively. HDAC1/3-IN-1 increases the SubG1 cell population and promotes apoptosis of glioma cells and glioblastoma stem cells. HDAC1/3-IN-1 can be used in studies related to glioblastoma.

For research use only. We do not sell to patients.

HDAC1/3-IN-1

HDAC1/3-IN-1 Chemical Structure

CAS No. : 244289-53-0

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Based on 1 publication(s) in Google Scholar

HDAC1/3-IN-1 Related Antibodies

Top Publications Citing Use of Products

View All HDAC Isoform Specific Products:

View All Isoforms
HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

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Description

HDAC1/3-IN-1 is a selective HDAC1/3 inhibitor, with IC50 values of 256 nM and 340.3 nM against HDAC1 and HDAC3, respectively. HDAC1/3-IN-1 increases the SubG1 cell population and promotes apoptosis of glioma cells and glioblastoma stem cells. HDAC1/3-IN-1 can be used in studies related to glioblastoma[1].

IC50 & Target[1]

HDAC1

256 nM (IC50)

HDAC3

340.3 nM (IC50)

In Vitro

HDAC1/3-IN-1 (Compound 6a) (0.6-50 μM; 24-72 h) inhibits the proliferation of HOG, T98G, U87MG and U251MG glioma cell lines in a time-dependent manner, with the lowest GI50 of 0.6 μM measured in U251MG cells after 72 h of incubation[1].
HDAC1/3-IN-1 (10.8-50 μM; 24 h) inhibits histone H3 deacetylation in HOG, T98G and U251MG glioma cell lines when applied at the relevant concentrations for 24 h[1].
HDAC1/3-IN-1 (10.8-50 μM; 24 h) induces SubG1 phase cell accumulation in aggressive glioma cell lines T98G and U251MG, suggesting the presence of DNA fragmentation and apoptosis, while also modulating the cell cycle phase distribution of the tested glioma cell lines[1].
HDAC1/3-IN-1 (10.8-50 μM; 24 h) induces early apoptosis in glioma cell lines HOG, T98G, U87MG and U251MG[1].
HDAC1/3-IN-1 (72 h) inhibits the viability of mesenchymal (GG16) and proneural (GSC23) glioblastoma stem cell lines, with IC50 values of 2.079 μM and 1.851 μM respectively after 72 h of incubation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HDAC1/3-IN-1 Related Antibodies

Western Blot Analysis[1]

Cell Line: Glioma cell lines HOG, T98G, U87MG, U251MG
Concentration: 50 μM (HOG, T98G, U87MG); 10.8 μM (U251MG)
Incubation Time: 24 h
Result: Promoted accumulation of acetyl-histone H3 in HOG, T98G, and U251MG cell lines, with comparable effects to positive controls in these cells.

Cell Cycle Analysis[1]

Cell Line: Glioma cell lines HOG, T98G, U87MG, U251MG
Concentration: 50 μM (T98G); 10.8 μM (U251MG)
Incubation Time: 24 h
Result: Increased the percentage of cells in the SubG1 phase in T98G and U251MG cell lines.
Modulated cell cycle phase distribution, increasing the G0/G1 phase in HOG cells and modulating the S phase in U87MG cells.

Apoptosis Analysis[1]

Cell Line: Glioma cell lines HOG, T98G, U87MG, U251MG
Concentration: 50 μM (HOG, T98G, U87MG); 10.8 μM (U251MG)
Incubation Time: 24 h
Result: Induced early apoptosis in HOG, T98G, U87MG, and U251MG cell lines.
Molecular Weight

367.42

Formula

C19H17N3O3S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC=CC=C1N)C2=CC=C(NS(=O)(C3=CC=CC=C3)=O)C=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (136.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7217 mL 13.6084 mL 27.2168 mL
5 mM 0.5443 mL 2.7217 mL 5.4434 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7217 mL 13.6084 mL 27.2168 mL 68.0420 mL
5 mM 0.5443 mL 2.7217 mL 5.4434 mL 13.6084 mL
10 mM 0.2722 mL 1.3608 mL 2.7217 mL 6.8042 mL
15 mM 0.1814 mL 0.9072 mL 1.8145 mL 4.5361 mL
20 mM 0.1361 mL 0.6804 mL 1.3608 mL 3.4021 mL
25 mM 0.1089 mL 0.5443 mL 1.0887 mL 2.7217 mL
30 mM 0.0907 mL 0.4536 mL 0.9072 mL 2.2681 mL
40 mM 0.0680 mL 0.3402 mL 0.6804 mL 1.7011 mL
50 mM 0.0544 mL 0.2722 mL 0.5443 mL 1.3608 mL
60 mM 0.0454 mL 0.2268 mL 0.4536 mL 1.1340 mL
80 mM 0.0340 mL 0.1701 mL 0.3402 mL 0.8505 mL
100 mM 0.0272 mL 0.1361 mL 0.2722 mL 0.6804 mL
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HDAC1/3-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HDAC1/3-IN-1244289-53-0HDACApoptosisHistone deacetylasesclass IIb HDAC isoformsglioblastoma stem cellsglioblastomaT98G cellsglioma cellsgliomaclass I HDAC isoformsHDAC3HOG cellsHDAC1Inhibitorinhibitorinhibit

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