2. HODHBt

HODHBt (HOOBt) inhibits STAT5-SUMO interaction by blocking SUMOylation of phosphorylated STAT5. HODHBt enhances the magnitude of IL-15 signaling and significantly increases the natural killer (NK) cell cytotoxicity phenotype and function and the generation of cytokine-induced memory-like (CIML) natural killer (NK) cells. HODHBt can be used for research of HIV-infection and cancer.

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HODHBt Chemical Structure

HODHBt Chemical Structure

CAS No. : 28230-32-2

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10 mM * 1 mL in DMSO
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HODHBt Related Antibodies

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Description

HODHBt (HOOBt) inhibits STAT5-SUMO interaction by blocking SUMOylation of phosphorylated STAT5. HODHBt enhances the magnitude of IL-15 signaling and significantly increases the natural killer (NK) cell cytotoxicity phenotype and function and the generation of cytokine-induced memory-like (CIML) natural killer (NK) cells. HODHBt can be used for research of HIV-infection and cancer[1].

In Vitro

HODHBt (100 μM; 24 h, and 48 h respectively) increases IL-15-mediated STAT phosphorylation in NK cells and enhances the cytotoxic profile of NK cells[1].
HODHBt (100 μM; 24 h) enhances IL-15-mediated (100 ng/mL) cytotoxicity of NK cells against HIV-infected cells (K562 cells)[1].
HODHBt (100 μM; 24 h) enhances IL-15-mediated (100 ng/mL, 6 h) cytotoxicity of NK cells against cancer cell lines (A2780 and U877; OCILy1 and OCILy10)[1].
HODHBt (100 μM; 7 d) results in enhanced memory response upon recall in generation of human CIML NK cells in vitro[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HODHBt Related Antibodies
Molecular Weight

163.13

Formula

C7H5N3O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1N(O)N=NC2=CC=CC=C12
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 500 mg/mL (3065.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.1301 mL 30.6504 mL 61.3008 mL
5 mM 1.2260 mL 6.1301 mL 12.2602 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation

Purity: 99.93%

COA (250 KB) HNMR (132 KB) RP-HPLC (192 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.1301 mL 30.6504 mL 61.3008 mL 153.2520 mL
5 mM 1.2260 mL 6.1301 mL 12.2602 mL 30.6504 mL
10 mM 0.6130 mL 3.0650 mL 6.1301 mL 15.3252 mL
15 mM 0.4087 mL 2.0434 mL 4.0867 mL 10.2168 mL
20 mM 0.3065 mL 1.5325 mL 3.0650 mL 7.6626 mL
25 mM 0.2452 mL 1.2260 mL 2.4520 mL 6.1301 mL
30 mM 0.2043 mL 1.0217 mL 2.0434 mL 5.1084 mL
40 mM 0.1533 mL 0.7663 mL 1.5325 mL 3.8313 mL
50 mM 0.1226 mL 0.6130 mL 1.2260 mL 3.0650 mL
60 mM 0.1022 mL 0.5108 mL 1.0217 mL 2.5542 mL
80 mM 0.0766 mL 0.3831 mL 0.7663 mL 1.9157 mL
100 mM 0.0613 mL 0.3065 mL 0.6130 mL 1.5325 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HODHBt28230-32-2HOOBtInhibitorinhibitorinhibit

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