IGN523
Based on 1 Customer Validation
IGN523 is an anti-CD98 antibody (hCD98, KD = 0.55 nM). IGN523 induces antibody-dependent cell-mediated cytotoxicity (ADCC) activity, lysosomal membrane permeabilization, and inhibition of essential amino acid transport, ultimately leading to caspase-3 and caspase-7-mediated apoptosis of tumor cells. IGN523 inhibits tumor growth in multiple tumor xenograft models. IGN523 is useful in the research of non-small cell lung cancer (NSCLC), acute myeloid leukemia (AML), and other cancers..
For research use only. We do not sell to patients.
- Purity: 97.47%
- Molecular Weight:145.63 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
Isotype
Human IgG1 kappa
Recommend Isotype Controls
Species Reactivity
Human
IC50 & Target
[1]|
Caspase 3 |
Caspase-7 |
In Vitro
IGN523 elicits strong ADCC activity in Ramos, KG-1 and OCI-AML-3 cells, with EC50s of 18.6 ng/mL, 73.8 ng/mL and 83.3 ng/mL, respectively, does not reduce ADCC activity in IGN523 cells[1].
IGN523 (2 µg/mL, 0.5-48 h) elicits Caspase-dependent apoptosis, increases lysosomal membrane permeability and decreases amino acid transport in Ramos, KG-1 and OCI-AML-3 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Ramos, KG-1 and OCI-AML-3 cells
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Concentration:2 µg/mL
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Incubation Time:48 h
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Result:Decreased cell viability in the presence of AbXL (10 µg/mL) (Ramos: 70% reduction; OCI-AML-3: 76% reduction; KG-1: 80% reduction) , but not when used alone.
Increased caspase-3 and caspase-7 activity (Ramos: 3.3-fold; OCI-AML-3: 7.5-fold and KG-1: 6.6-fold).
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Cell Line:Ramos cells
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Concentration:2 µg/mL
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Incubation Time:4℃ for 30 min
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Result:Showed the CD98-IGN523 complex localized to lysosome-associated membrane protein LAMP-1 structures, increased the rate of internalization, capped CD98 at the cell surface.
In Vivo
IGN523 (15 mg/kg, i.p., once a week, twice/three times/four times) inhibits tumor growth in AML and primary NSCLC xenograft mice models[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Ramos and Dau xenografts mice model[1]
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Dosage:15 mg/kg
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Administration:i.p., once a week, twice/three times
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Result:Inhibited tumor growth, with TGIs of 98%, 76 %, respectively.
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Animal Model:AML (HL-60, OCI-AML-3 (NOD/SCID) and KG-1 (1 x 107) and NSCLC (IGN-LNG-12 and IGN-LNG-54) (1 x 106) xenografts NOD/SCID or CB.17-SCID mice model[1]
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Dosage:15 mg/kg
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Administration:i.p., once a week, twice/three times/four times
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Result:Reduced tumor growth compared with isotype control in all 5 xenograft models, with TGIs of HL-60 (94%), OCI-AML-3 (74%), KG-1 (53%), IGN-LNG-12 (44%) and IGN-LNG-54 (56%), without inducing body weight loss. Completely eliminated CD98 expression and CD98+-tumor cells.
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Gene ID
Accession
AAH01061
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Application
ELISA, FACS, Functional assay
Verified Bioactivity
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Immobilized Human CD98 Protein, hFC Tag can bind IGN523. The EC50 for this effect is 80.8 ng/mL.
Chemical Information
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Appearance Liquid
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Molecular Weight 145.63 kDa
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Color Colorless to light yellow
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SMILES
N/A
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Synonyms
AML-01
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (262 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
IGN523 Related Classifications
HY-P991669 Related Classifications
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