ISX-1
Based on 1 Customer Validation
ISX-1 is an isoxazole. ISX-1 has anti-adipogenic and pro-osteogenic activities. ISX-1 can be used for the research of osteopenia and osteoporosis.
For research use only. We do not sell to patients.
- Purity: 98.05%
- CAS No.: 909207-35-8
- Formula: C14H14N4O2S
- Molecular Weight:302.35
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Cell Cycle/DNA Damage Compound Library
HY-L004
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Metabolism/Protease Compound Library
HY-L012
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Anti-Aging Compound Library
HY-L034
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Anti-Obesity Compound Library
HY-L087
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Transcription Factor-Targeted Library
HY-L090
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Lipid Metabolism Compound Library
HY-L091
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Nuclear Receptor Compound Library
HY-L126
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Osteogenesis Compound Library
HY-L131
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Metabolic Enzyme Compound Library
HY-L146
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Multi-Target Compound Library
HY-L176
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
IC50: 1.9 μM (lipid droplet formation); EC50: 1.2 μM (induce ALP)[1]
ISX-1 dose-dependently inhibits the accumulation of intracellular lipid droplets and stimulated ALP activity[1].
ISX-1 can form lipid droplet with an IC50 value of 1.9 μM and also can induce ALP with an EC50 value of 1.2 μM[1].
ISX-1 (0, 1.3, 6.5 and 33 μM; 3 days) inhibited the mRNA induction of PPARγ and FABP4 genes under the adipogenic differentiation of hBMSCs[1].
ISX-1 (0, 1.3, 6.5 and 33 μM) promotes TCF/LEF-mediated gene transcription[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human bone marrow mesenchymal stem cells (hBMSCs)
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Concentration:0, 1.3, 6.5 and 33 μM
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Incubation Time:3 days
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Result:Inhibited the mRNA induction of PPARγ and FABP4 genes under the adipogenic differentiation of hBMSCs and showed no effect on C/EBPα expression.
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Cell Line:HEK 293 cells
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Concentration:6.5 and 33 μM
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Incubation Time:6.5 and 33 μM
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Result:Increased both the amount of activated and total β-catenin.
Chemical Information
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CAS No. 909207-35-8
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Appearance Solid
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Molecular Weight 302.35
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Formula C14H14N4O2S
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Color White to light yellow
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SMILES
O=C(NCCCN1C=CN=C1)C2=NOC(C3=CC=CS3)=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 50 mg/mL (165.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3074 mL | 16.5371 mL | 33.0743 mL | 82.6856 mL |
| 5 mM | 0.6615 mL | 3.3074 mL | 6.6149 mL | 16.5371 mL | |
| 10 mM | 0.3307 mL | 1.6537 mL | 3.3074 mL | 8.2686 mL | |
| 15 mM | 0.2205 mL | 1.1025 mL | 2.2050 mL | 5.5124 mL | |
| 20 mM | 0.1654 mL | 0.8269 mL | 1.6537 mL | 4.1343 mL | |
| 25 mM | 0.1323 mL | 0.6615 mL | 1.3230 mL | 3.3074 mL | |
| 30 mM | 0.1102 mL | 0.5512 mL | 1.1025 mL | 2.7562 mL | |
| 40 mM | 0.0827 mL | 0.4134 mL | 0.8269 mL | 2.0671 mL | |
| 50 mM | 0.0661 mL | 0.3307 mL | 0.6615 mL | 1.6537 mL | |
| 60 mM | 0.0551 mL | 0.2756 mL | 0.5512 mL | 1.3781 mL | |
| 80 mM | 0.0413 mL | 0.2067 mL | 0.4134 mL | 1.0336 mL | |
| 100 mM | 0.0331 mL | 0.1654 mL | 0.3307 mL | 0.8269 mL |
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