ISX-3
Based on 1 Customer Validation
ISX-3 is a potent anti-adipogenic and pro-osteogenic agent. ISX-3 increases the expression of PPARγ. ISX-3 has the potential for the research of osteopenia and osteoporosis.
For research use only. We do not sell to patients.
- Purity: 99.68%
- CAS No.: 912789-08-3
- Formula: C16H15ClN4O2
- Molecular Weight:330.77
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Cell Cycle/DNA Damage Compound Library
HY-L004
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Metabolism/Protease Compound Library
HY-L012
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Anti-Aging Compound Library
HY-L034
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Transcription Factor-Targeted Library
HY-L090
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Nuclear Receptor Compound Library
HY-L126
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Osteogenesis Compound Library
HY-L131
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Mitochondrial Protection Compound Library
HY-L144
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Metabolic Enzyme Compound Library
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
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Bioactive Compound Library
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Anti-Fibrosis Compound Library
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
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High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
ISX-3 (0-100,000 nM; 10 days) shows a stimulatory effect on the accumulation of lipid droplets and an inhibitory effect on ALP (alkaline phosphatase) induction[1].
ISX-3 (50 μM; 2 days) increases the expression of PPARγ in the presence of MDI in hBMSCs[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:hBMSCs
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Concentration:50 µM
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Incubation Time:2 days
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Result:Increased the PPARγ expression level in the presence of MDI (0.5 mM isobutyl methylxanthine, 1 μM dexamethasone and 1 μM insulin).
Chemical Information
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CAS No. 912789-08-3
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Appearance Solid
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Molecular Weight 330.77
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Formula C16H15ClN4O2
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Color Off-white to light yellow
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SMILES
O=C(C1=NOC(C2=CC(Cl)=CC=C2)=C1)NCCCN3C=NC=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (302.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.56 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (7.56 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0232 mL | 15.1162 mL | 30.2325 mL | 75.5812 mL |
| 5 mM | 0.6046 mL | 3.0232 mL | 6.0465 mL | 15.1162 mL | |
| 10 mM | 0.3023 mL | 1.5116 mL | 3.0232 mL | 7.5581 mL | |
| 15 mM | 0.2015 mL | 1.0077 mL | 2.0155 mL | 5.0387 mL | |
| 20 mM | 0.1512 mL | 0.7558 mL | 1.5116 mL | 3.7791 mL | |
| 25 mM | 0.1209 mL | 0.6046 mL | 1.2093 mL | 3.0232 mL | |
| 30 mM | 0.1008 mL | 0.5039 mL | 1.0077 mL | 2.5194 mL | |
| 40 mM | 0.0756 mL | 0.3779 mL | 0.7558 mL | 1.8895 mL | |
| 50 mM | 0.0605 mL | 0.3023 mL | 0.6046 mL | 1.5116 mL | |
| 60 mM | 0.0504 mL | 0.2519 mL | 0.5039 mL | 1.2597 mL | |
| 80 mM | 0.0378 mL | 0.1890 mL | 0.3779 mL | 0.9448 mL | |
| 100 mM | 0.0302 mL | 0.1512 mL | 0.3023 mL | 0.7558 mL |
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