3-Hydroxybenzaldehyde
Based on 1 Customer Validation
3-Hydroxybenzaldehyde (3-HBA) is a precursor compound for phenolic compounds like Protocatechuic aldehyde (PCA) (HY-N0295). 3-Hydroxybenzaldehyde, produced by 3-hydroxybenzyl-alcohol dehydrogenase, is a substrate of aldehyde dehydrogenase (ALDH) in rats and humans. 3-Hydroxybenzaldehyde has vasculoprotective effects in vitro and in vivo. 3-Hydroxybenzaldehyde is proming for research of atherosclerosis.
For research use only. We do not sell to patients.
- Purity: 99.89%
- CAS No.: 100-83-4
- Formula: C7H6O2
- Molecular Weight:122.12
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Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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ALDH2 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MDA-MB-231 | IC50 |
8.2 μM
Compound: 21
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Growth inhibition of human MDA-MB-231 cells after 72 hrs by alamar blue assay
Growth inhibition of human MDA-MB-231 cells after 72 hrs by alamar blue assay
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[PMID: 28001067] |
3-Hydroxybenzaldehyde (0-100 μM, 24 h) inhibits vascular smooth muscle cells (VSMC) proliferation, cell cycle and cell migration[1].
3-Hydroxybenzaldehyde (100 μM, 25 h) inhibits production levels of p-NF-κB and p-p38 increased by TNF-α in human umbilical vein endothelial cells (HUVECs)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Rat vascular smooth muscle cells (VSMCs)
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Concentration:0, 25, 50, 100 μM
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Incubation Time:48 h or 144 h
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Result:Showed low toxicity in VSMC for both 48 and 144 h.
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Cell Line:Rat vascular smooth muscle cells (VSMCs)
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Concentration:100 μM
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Incubation Time:24 h
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Result:Arrested the S phase and G0/G1 phase increased by PDGF and lowered the expression levels of cyclin D1 (CD1) and retinoblastoma (Rb1) mRNA in VSMCs.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:6-week-old male SD rats[1]
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Dosage:100 mg/kg
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Administration:i.p., daily for 1 week
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Result:Exhibited anti-thrombic effects in vivo.
Chemical Information
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CAS No. 100-83-4
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Appearance Solid
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Molecular Weight 122.12
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Formula C7H6O2
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Color Off-white to light yellow
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SMILES
O=CC1=CC=CC(O)=C1
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Synonyms
M-Hydroxybenzaldehyde
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
DMSO : 100 mg/mL (818.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Kong BS, et al. Vasculoprotective Effects of 3-Hydroxybenzaldehyde against VSMCs Proliferation and ECs Inflammation.PLoS One. 2016 Mar 22;11(3):e0149394. [Content Brief]
[2]. Paixão JA, et al. 3-Hydroxybenzaldehyde. Acta Crystallogr C. 2000 Nov;56 Pt 11:1348-50. [Content Brief]
[3]. Chen K Y, et al. Protective effect of benzaldehyde combined with albendazole against brain injury induced by Angiostrongylus cantonensis infection in mice[J]. International Journal of Antimicrobial Agents, 2023, 62(5): 106963. [Content Brief]
[4]. Muthu S, et al. Quantum chemical studies, vibrational analysis, molecular structure, first order hyper polarizability, NBO and HOMO-LUMO analysis of 3-hydroxybenzaldehyde and its cation[J]. Spectrochim Acta A Mol Biomol Spectrosc. 2013 Nov;115:789-99. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 8.1887 mL | 40.9433 mL | 81.8867 mL | 204.7167 mL |
| 5 mM | 1.6377 mL | 8.1887 mL | 16.3773 mL | 40.9433 mL | |
| 10 mM | 0.8189 mL | 4.0943 mL | 8.1887 mL | 20.4717 mL | |
| 15 mM | 0.5459 mL | 2.7296 mL | 5.4591 mL | 13.6478 mL | |
| 20 mM | 0.4094 mL | 2.0472 mL | 4.0943 mL | 10.2358 mL | |
| 25 mM | 0.3275 mL | 1.6377 mL | 3.2755 mL | 8.1887 mL | |
| 30 mM | 0.2730 mL | 1.3648 mL | 2.7296 mL | 6.8239 mL | |
| 40 mM | 0.2047 mL | 1.0236 mL | 2.0472 mL | 5.1179 mL | |
| 50 mM | 0.1638 mL | 0.8189 mL | 1.6377 mL | 4.0943 mL | |
| 60 mM | 0.1365 mL | 0.6824 mL | 1.3648 mL | 3.4119 mL | |
| 80 mM | 0.1024 mL | 0.5118 mL | 1.0236 mL | 2.5590 mL | |
| 100 mM | 0.0819 mL | 0.4094 mL | 0.8189 mL | 2.0472 mL |
3-Hydroxybenzaldehyde Related Classifications
HY-76006 Related Classifications
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