TPE-MI
Based on 18 publication(s) in Google Scholar
TPE-MI (Tetraphenylethene maleimide) is a thiol probe for measuring unfolded protein load and proteostasis in cells (the excitation wavelength is 350 nm and the emission wavelength is 470 nm). TPE-MI can report imbalances in proteostasis in induced pluripotent stem cell models of Huntington disease, as well as cells transfected with mutant Huntington exon 1 before the formation of visible aggregates. TPE-MI also detects protein damage following dihydroartemisinin research of the malaria parasites Plasmodium falciparum .
For research use only. We do not sell to patients.
- Purity: 98.93%
- CAS No.: 1245606-71-6
- Formula: C31H23NO2
- Molecular Weight:441.52
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Storage:
4°C, protect from light
* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) TPE-MI
More- Cell Metab. 2026 Jun 24:S1550-4131(26)00226-3. [Abstract]
- Cancer Commun (Lond). 2025 Nov 10. [Abstract]
- Mol Cell. 2026 Mar 3:S1097-2765(26)00102-4. [Abstract]
- Nat Commun. 2026 Apr 22;17(1):5596. [Abstract]
- Nat Commun. 2025 Jul 24;16(1):6821. [Abstract]
- J Adv Res. 2025 May 25:S2090-1232(25)00370-4. [Abstract]
- Sci Adv. 2025 Dec 5;11(49):eaea7451. [Abstract]
- Sci China Life Sci. 2025 Feb;68(2):416-430. [Abstract]
- Cell Commun Signal. 2025 Sep 3;23(1):393. [Abstract]
- Free Radic Biol Med. 2026 Mar 26:S0891-5849(26)00250-9. [Abstract]
- Cell Biosci. 2024 Jun 10;14(1):78. [Abstract]
- NPJ Aging. 2026 Mar 5. [Abstract]
- Matrix Biol. 2026 Jun:146:102011. [Abstract]
- Exp Neurol. 2025 Apr 26:115273. [Abstract]
- Res Sq. 2026 Jun 18.
- bioRxiv. 2025 Sep 9.
- SSRN. 2025 Jan 27.
- bioRxiv. 2024 Mar 16.
Customer Validation & Images
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Flow Cytometry
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Flow Cytometry
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Bio/Physico-chemical Assay
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Flow Cytometry
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Flow Cytometry
All Parasite Isoforms
More
Biological Activity
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Plasmodium |
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Preparation of Dye Stock Solution[1]
1.1 Dissolve TPE-MI in DMSO to prepare a stock solution.
Note: It is recommended to aliquot the TPE-MI stock solution and store it at -20℃ or -80℃ protected from light.
2. Preparation of Dye Working Solution
2.1 Dilute the TPE-MI stock solution to a working concentration of 50 μM with PBS.
Note: Please adjust the concentration of the TPE-MI working solution according to the actual situation, and prepare it fresh for each use.
3. Specific Staining Steps
3.1 Cell Culture Preparation: Seed the cells in a suitable culture container.
3.2 Washing: Wash cells with PBS.
3.3 Staining: Add 50 μM TPE-MI working solution to the cells and incubate at 37 °C for 30 min.
4. Detection
Flow Cytometry Detection: Remove the TPE-MI solution, digest the cells, resuspend in PBS, centrifuge, discard the supernatant, and resuspend the cells in PBS for analysis.
SDS-PAGE Detection: Lyse stained cells with cell lysis buffer, determine protein concentration, and perform SDS-PAGE electrophoresis followed by fluorescence and Coomassie Brilliant Blue staining analysis.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 1245606-71-6
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Appearance Solid
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Molecular Weight 441.52
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Formula C31H23NO2
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Color Light yellow to yellow
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SMILES
O=C(C=C1)N(C2=CC=C(/C(C3=CC=CC=C3)=C(C4=CC=C(C)C=C4)/C5=CC=CC=C5)C=C2)C1=O
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Synonyms
Tetraphenylethene maleimide
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)
Publications (18)
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Journal Impact Factor
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Most Recent
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Cell Metab
Gut microbiota-derived lysine phenylacetylation impairs mitochondrial function and is alleviated by SIRT3. [Abstract]2026 Jun 24:S1550-4131(26)00226-3. PMID: 42341761 -
Cancer Commun (Lond)
Unfolded protein response kinase PERK supports survival and metastasis of circulating tumor cell clusters via SAM synthesis and H3K4me3-dependent PDGFB signaling. [Abstract]2025 Nov 10. PMID: 41212905
TPE-MI purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 Nov 10. [Abstract]
MFI of TPE‐MI in single CTCs and CTC clusters, as determined by flow cytometry (n = 5).
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Mol Cell
Global profiling of nascent chain interactors reveals TRIM25 as a co-translational E3 ubiquitin ligase. [Abstract]2026 Mar 3:S1097-2765(26)00102-4. PMID: 41780531 -
Nat Commun
Transcription factor 19 modulates fatty acid elongation and unfolded protein response to attenuate palmitic acid-induced hepatic dysfunction. [Abstract]2026 Apr 22;17(1):5596. PMID: 42020413 -
Nat Commun
Impairing the interaction between Erg11 and cytochrome P450 reductase Ncp1 enhances azoles' antifungal activities. [Abstract]2025 Jul 24;16(1):6821. PMID: 40707518
TPE-MI purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 24;16(1):6821. [Abstract]
TPE-MI (50 μM, 30 min). Misfolded proteins in ERG11/erg11Δ mutants were analyzed after treatment with 16 μg/mL ellipticine and 4 μg/mL FLC.
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J Adv Res
Fibrinogen exacerbates α-synuclein aggregation and mitochondrial dysfunction via alpha5beta3 integrin in Parkinson's disease. [Abstract]2025 May 25:S2090-1232(25)00370-4. PMID: 40425084
TPE-MI purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 May 25:S2090-1232(25)00370-4. [Abstract]
Mitochondrial unfolded protein loading was measured by TPE-MI (50 µM) fluorescence for 2 h, and the fluorescence intensity at 2 h was compared among the groups (three independent replicates).
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Sci Adv
Rbfox2 selectively governs hematopoietic stem cell self-renewal by regulating proteostasis. [Abstract]2025 Dec 5;11(49):eaea7451. PMID: 41337583
TPE-MI purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Dec 5;11(49):eaea7451. [Abstract]
Misfolded/unfolded protein contents in control and Rbfox2-deficient HSCs assessed with TPE-MI (150 μM, at 37°C for 45 min) dye. Representative histograms of TPE-MI intensity are shown in. Quantification is shown in.
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Sci China Life Sci
Tauroursodeoxycholic acid targets HSP90 to promote protein homeostasis and extends healthy lifespan. [Abstract]2025 Feb;68(2):416-430. PMID: 39327392 -
Cell Commun Signal
Targeting mitochondrial proteases CLPP and LONP1 via disruption of mitochondrial redox homeostasis induces proteotoxic stress and suppresses tumor progression. [Abstract]2025 Sep 3;23(1):393. PMID: 40903791
TPE-MI purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Sep 3;23(1):393. [Abstract]
Effect of MC on cellular protein homeostasis in MDA-MB-231 cells. Each bar indicates the mean ± SEM of fluorescence intensity of TPE-MI-stained (50 μM) cells from three replicates and 2 independent experiments were performed.
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Free Radic Biol Med
Lonp1 inhibition disrupts mitochondrial function in the hippocampus and drives an aging-like synaptic and cognitive phenotype in adult SAMP8 mice. [Abstract]2026 Mar 26:S0891-5849(26)00250-9. PMID: 41903616 -
Cell Biosci
2024 Jun 10;14(1):78. PMID: 38858714 -
NPJ Aging
Early mitophagy activation by Urolithin A prevents, but late activation does not reverse, age-related cognitive impairment. [Abstract]2026 Mar 5. PMID: 41786716 -
Matrix Biol
C-terminally clustered UGDH hypomorphic variants reveal subtle mechanisms of cellular and developmental disruption. [Abstract]2026 Jun:146:102011. PMID: 42061477 -
Exp Neurol
SDF2L1 downregulation mediates high glucose-caused Schwann cell dysfunction by inhibiting nuclear import of TFEB and CREB via KPNA3. [Abstract]2025 Apr 26:115273. PMID: 40294738 -
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Solvent & Solubility
DMSO : 8.57 mg/mL (19.41 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (281 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Chen MZ, et al. A thiol probe for measuring unfolded protein load and proteostasis in cells. Nat Commun. 2017;8(1):474. Published 2017 Sep 7. [Content Brief]
[2]. Hu Q, et al. In Situ Monitored Vortex Fluidic-Mediated Protein Refolding/Unfolding Using an Aggregation-Induced Emission Bioprobe. Molecules. 2021;26(14):4273. Published 2021 Jul 14. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.2649 mL | 11.3245 mL | 22.6490 mL | 56.6226 mL |
| 5 mM | 0.4530 mL | 2.2649 mL | 4.5298 mL | 11.3245 mL | |
| 10 mM | 0.2265 mL | 1.1325 mL | 2.2649 mL | 5.6623 mL | |
| 15 mM | 0.1510 mL | 0.7550 mL | 1.5099 mL | 3.7748 mL |
TPE-MI Related Classifications
HY-143218 Related Classifications
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