Azomycin
Based on 1 Customer Validation
Azomycin (2-Nitroimidazole) is an antibiotic which can be active against aerobic Gram-positive and Gram-negative bacteria.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.17%
- CAS 番号: 527-73-1
- 分子式: C3H3N3O2
- 分子量:113.08
-
保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
-
溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
-
HY-N0195R
Azomycin (Standard)Bacterial Inhibitor -
HY-B0490
Hygromycin BAntibiotic -
HY-B0271
PyrazinamideAntitubercular Antibiotic -
HY-10844
PretomanidAntibiotic -
HY-76299
GalanthamineCholinesterase (ChE) Inhibitor -
HY-B0271R
Pyrazinamide (Standard)Antitubercular Antibiotic -
HY-10844R
Pretomanid (Standard)Antibiotic -
HY-B0535A
Ethambutol dihydrochlorideBacteriostatic Antimycobacterial Agent -
HY-B0490R
Hygromycin B (Standard)Antibiotic -
HY-41461
5-Amino-3H-imidazole-4-CarboxamideEndogenous Metabolite -
HY-A0009
Galanthamine hydrobromideAChE Inhibitor -
HY-W053787
1-Methylhistamine dihydrochloride
Antibiotic アイソフォーム固有の製品をすべて表示
More
生物活性
製品説明
IC50 & Target
Bacterial[1]
体外実験
Azomycin is an antibiotic which can be active against aerobic Gram-positive and Gram-negative bacteria. It is found that Azomycin is also active against a variety of anaerobic bacteria[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
化学情報
-
CAS 番号 527-73-1
-
性状 Solid
-
分子量 113.08
-
分子式 C3H3N3O2
-
Color White to yellow
-
SMILES
O=[N+]([O-])C1=NC=CN1
-
別名
2-Nitroimidazole
-
Structure Classification
-
Initial Source
Nocardia mesenterica
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
溶剤 & 溶解度
体外:
DMSO : 50 mg/mL (442.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.67 mg/mL (23.61 mM); Clear solution
This protocol yields a clear solution of ≥ 2.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (26.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
プロトコル
-
Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.
純度とドキュメンテーション
-
データシート (274 KB)
-
SDS (481 KB)
- English - EN (481 KB)
- Français - FR (481 KB)
- Deutsch - DE (481 KB)
- Norwegian - NO (481 KB)
- Español - ES (481 KB)
- Swedish - SV (481 KB)
- Italian - IT (481 KB)
- Korean - KR (481 KB)
- Portuguese - PT (481 KB)
-
取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 8.8433 mL | 44.2165 mL | 88.4330 mL | 221.0824 mL |
| 5 mM | 1.7687 mL | 8.8433 mL | 17.6866 mL | 44.2165 mL | |
| 10 mM | 0.8843 mL | 4.4216 mL | 8.8433 mL | 22.1082 mL | |
| 15 mM | 0.5896 mL | 2.9478 mL | 5.8955 mL | 14.7388 mL | |
| 20 mM | 0.4422 mL | 2.2108 mL | 4.4216 mL | 11.0541 mL | |
| 25 mM | 0.3537 mL | 1.7687 mL | 3.5373 mL | 8.8433 mL | |
| 30 mM | 0.2948 mL | 1.4739 mL | 2.9478 mL | 7.3694 mL | |
| 40 mM | 0.2211 mL | 1.1054 mL | 2.2108 mL | 5.5271 mL | |
| 50 mM | 0.1769 mL | 0.8843 mL | 1.7687 mL | 4.4216 mL | |
| 60 mM | 0.1474 mL | 0.7369 mL | 1.4739 mL | 3.6847 mL | |
| 80 mM | 0.1105 mL | 0.5527 mL | 1.1054 mL | 2.7635 mL | |
| 100 mM | 0.0884 mL | 0.4422 mL | 0.8843 mL | 2.2108 mL |
Azomycin 関連分類
HY-N0195 関連分類
Powered by Bioz