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Nutlin-3a

(別名: Rebemadlin)
74 Cited Publications
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Based on 74 publication(s) in Google Scholar

Nutlin-3a (Rebemadlin), an active enantiomer of Nutlin-3, is a potent murine double minute (MDM2) inhibitor (IC50=90 nM). Nutlin-3a inhibits MDM2-p53 interactions and stabilizes the p53 protein, and induces cell autophagy and apoptosis. Nutlin-3a has the potential for the study of TP53 wild-type ovarian carcinomas.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
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  • 純度: 99.13%
  • CAS 番号: 675576-98-4
  • 分子式: C30H30Cl2N4O4
  • 分子量:581.49
  • 保管条件:
    Powder   -20°C, 3 years ; In solvent   -80°C, 1 year , -20°C, 6 months
  • Nutlin-3a Chemical Structure
    Nutlin-3a 構造式
  • Experimental Validation Results for Nutlin-3a
  • Experimental Validation Results for Nutlin-3a
  • Experimental Validation Results for Nutlin-3a
  • Experimental Validation Results for Nutlin-3a
  • Experimental Validation Results for Nutlin-3a
  • Experimental Validation Results for Nutlin-3a
  • Experimental Validation Results for Nutlin-3a
CAS 番号: 675576-98-4
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WBCell Proliferation/Viability AssayRT-PCRIPIF
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  • Cell Proliferation/Viability Assay
  • RT-PCR
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  • Nutlin-3a Chemical Structure
    Nutlin-3a Chemical Structure
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  • 1/16

    Nutlin-3a purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 May 13;42(5):850-868.e9. [Abstract]

    Customer Validation
     Immunoblotting of Eμ-Myc lymphoma (AF47A/560) and DHL (214DHL) cell lines pre-treated with QVD-O-Ph then S63845, venetoclax, or Nutlin-3a for indicated time points. HSP70 was used as a loading control.

  • 1/16

    Nutlin-3a purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 May 13;42(5):850-868.e9. [Abstract]

    Customer Validation
     Cell viability assays of NTC and TP53 KO human B cell lymphoma cell lines. Cells were treated with the p53-activating drug Nutlin-3a for 24 h. Cell viability was measured by Annexin V/PI staining followed by flow cytometry. Error bars represent S.D. for three independent experiments. Student’s t-test; **=p<0.01, *=p<0.05.

  • 1/16

    Nutlin-3a purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 May 13;42(5):850-868.e9. [Abstract]

    Customer Validation
     RTqPCR for the p53 target genes PUMA and P21 in parental DOHH2 and two independently derived Rho0 cell lines. Cells were pre-treated with the caspase inhibitor QVD-O-Ph (HY-12305), and then treated with 100 nM S63845 for 24 h or, as a comparator for potent p53 activation, treated with 10 µM of the MDM2 inhibitor Nutlin-3a (HY-10029) for 24 h. Ct values for each gene are normalized to the housekeeping gene HMBS, and fold change is shown relative to the DMSO-treated parental cell sample. Error bars represent S.E.M. for two independent experiments.

  • 1/16

    Nutlin-3a purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 May 13;42(5):850-868.e9. [Abstract]

    Customer Validation
     Immunoblotting for p53 and PUMA in parental DOHH2 lymphoma cells and two independently derived Rho0 cell lines. Cells were pre-treated with QVD-O-Ph (HY-12305), and then treated with 100 nM S63845 or, as a control for potent p53 stabilization, treated with 10 µM Nutlin-3a (HY-10029) for 24 h. Blotting for HSP70 was used as a loading control. Representative blot shown from three independent experiments. Quantifications of p53 protein levels from three independent immunoblotting experiments are shown, relative to HSP70. Error bars represent S.D.

  • 1/16

    Nutlin-3a purchased from MedChemExpress. Usage Cited in: R Soc Open Sci. 2018 Aug 29;5(8):172376. [Abstract]

    Customer Validation
     HEKs are transfected with CRISPR/CAS9-gRNA for miR-143 or miR-145 for 48 h and then treated with nutlin-3a and Puromycin for another 24 h. The protein expression of NRAS and target genes in the PI3 K/ATK pathway is analysed by western blotting.

  • 1/16

    Nutlin-3a purchased from MedChemExpress. Usage Cited in: J Mater Chem B. 2017 Aug 7;5(29):5816-5834. [Abstract]

    Customer Validation
     Immunofluorescence staining of p53, caspase 3, and MDM2 proteins in HepG2 cells treated with microparticles loaded with nutlin-3a and β-CD-g-CS/pDNA 5 days after commencement of the treatment.

  • 1/16

    Nutlin-3a purchased from MedChemExpress. Usage Cited in: Eur J Med Chem. 2016 May 23:114:328-36. [Abstract]

    Customer Validation
     The effects of compounds A1, A7, A9 or Nutlin-3a in HCT116 cells by immunoprecipitation analysis

  • Nutlin-3a