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製品番号: HY-D3169

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IETDC

IETDC is a caspase 8-responsive fluorescent probe. IETDC is cleaved by activated caspase 8 to release D-cysteine, which then reacts with 6-hydroxy-2-cyanobenzothiazole (HCBT) to form firefly luciferin in situ. IETDC must be used in conjunction with PCL-2 (HY-D3168) to detect the co-existence of hydrogen peroxide and caspase 8. IETDC is applicable to studies related to acute inflammation.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号: 1476735-95-1
分子式: C₃₂H₄₇N₅O₁₃S
分子量:741.81

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

カスタムペプチド

IETDC 構造式

CAS 番号: 1476735-95-1

価格（税別）

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性
- 製品説明
- IC₅₀ & Target
- 体外実験
- 体内実験
化学情報
純度とドキュメンテーション
参考文献
Calculators
Help & FAQs
関連分類
Bioz

生物活性

製品説明

IC₅₀ & Target

^[1]

Caspase-8

Caspase アイソフォーム固有の製品をすべて表示 :

体外実験

IETDC (5 μM; 60 min) is selectively cleaved by recombinant caspase 8 but not caspase 3 or caspase 9 to release D-cysteine, which forms luciferin with HCBT to produce a ca. 27-fold increase in bioluminescent signal, and this response is fully inhibited by a pan-caspase inhibitor^[1].

IETDC (10 μM; 60 min) acts as part of an AND-type molecular logic gate, producing an ca. 18-fold increase in bioluminescent signal only when combined with PCL-2, hydrogen peroxide, and active caspase 8, with signal blocked by inhibition of either hydrogen peroxide-mediated PCL-2 cleavage or caspase 8-mediated IETDC cleavage^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

Mice

IETDC (0.05 μmol; i.p.; single dose), co-administered with HCBT, produces an 18-fold bioluminescent signal increase in female FVB-luc+ mice with lipopolysaccharide-induced acute inflammation, which is 34% attenuated by pre-treatment with a pan-caspase inhibitor^[1].

Mice

IETDC (0.05 μmol; i.p.; single dose), co-administered with PCL-2, produces a 2.7-fold bioluminescent signal increase in female FVB-luc+ mice with LPS (HY-D1056)-induced acute inflammation, which is 30% attenuated by co-treatment with ascorbic acid and a pan-caspase inhibitor^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

FVB-luc+ (female, 2-5 months old)^[1]
Dosage:

0.05 μmol (co-administered with 0.05 μmol HCBT)
Administration:

i.p.; single dose
Result:

Produced an 18-fold increase in bioluminescent signal in lipopolysaccharide-treated mice compared to saline-treated controls.
Caused a 34% attenuation of the elevated bioluminescent signal when pre-treated with z-VD(OMe)-OPh.

Animal Model:

FVB-luc+ (female, 2-5 months old)^[1]
Dosage:

0.05 μmol (co-administered with 0.05 μmol PCL-2)
Administration:

i.p.; single dose
Result:

Produced a 2.7-fold increase in bioluminescent signal in lipopolysaccharide-treated mice compared to saline-treated controls.
Caused a 30% attenuation of the elevated bioluminescent signal when co-treated with ascorbic acid and z-VD(OMe)-OPh.

Related Protocols :

化学情報

CAS 番号 1476735-95-1
分子量 741.81
分子式 C₃₂H₄₇N₅O₁₃S
配列

Cbz-Ile-{Glu(Me)}-Thr-{Asp(Me)}-{d-Cys}
シーケンスの短縮

Cbz-I-{Glu(Me)}-T-{Asp(Me)}-{d-Cys}
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

取扱説明書 (2659 KB)

参考文献

[1]. Van de Bittner GC, et al. Strategy for dual-analyte luciferin imaging: in vivo bioluminescence detection of hydrogen peroxide and caspase activity in a murine model of acute inflammation. J Am Chem Soc. 2013;135(5):1783-1795. [Content Brief]

Calculators

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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