Ro 90-7501
Based on 3 publication(s) in Google Scholar
Ro 90-7501 is an amyloid β42 (Aβ42) fibril assembly inhibitor that reduces Aβ42-induced cytotoxicity (EC50 of 2 μM). Ro 90-7501 inhibits ATM phosphorylation and DNA repair. RO 90-7501 selectively enhances toll-like receptor 3 (TLR3) and RIG-I-like receptor (RLR) ligand-induced IFN-β gene expression and antiviral response. Ro 90-7501 also inhibits protein phosphatase 5 (PP5) in a TPR-dependent manner. Ro 90-7501 has significant radiosensitizing effects on cervical cancer cells.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 98.50%
- CAS 番号: 293762-45-5
- 分子式: C20H16N6
- 分子量:340.38
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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Ro 90-7501 (Standard)Reference Standard
MedChemExpress（MCE）の使用を引用している文献 Ro 90-7501
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Customer Validation & Images
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Cell Proliferation/Viability Assay
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IF
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Cell Migration/Invasion Assay
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Apoptosis Analysis
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WB
生物活性
製品説明
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Amyloid β42 |
ATM |
Protein phosphatase 5 |
Apoptosis |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Huh-7 | CC50 |
>100 μM
Compound: GNF-Pf-5510
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NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)
NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)
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[PMID: 18579783] |
体外実験
Ro 90-7501 significantly enhances radiosensitivity compared with control HeLa and ME-180 cells. Ro 90-7501 significantly increases apoptosis and impaired cell cycle after irradiation. Ro 90-7501 suppresses the phosphorylation of ATM and its downstream proteins, such as H2AX, Chk1, and Chk2, after irradiation[1].
RO 90-7501, itself affects neither IFN-β nor NFκB promoter activity, but significantly enhances poly I:C-induced IFN-β promoter activation and inhibits the activation of NFκB in a dose-dependent manner. Treatment of cells with RO 90-7501 significantly enhances the antiviral activity of poly I:C[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
体内実験
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female BALB/c nude mice (8-week-old) with HeLa cells under irradiation[1]
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Dosage:5 μg/g
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Administration:Intraperitoneal injection; daily; for 21 days
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Result:Tumor growth was significantly delayed in the combination group. Tumor volume was also significantly decreased in the irradiation group.
- Kinase activity and phosphorylation assays
- RT-PCR
- Western Blot
- RNA extraction experimental
- Research Protocol for Infectious Diseases
- Cell Cytotoxicity Assay
- Mammalian live/dead viability and cytotoxicity staining
- Protocol for Kinase activity and phosphorylation assays
- Amyloid: Congo Red Amyloid Staining
- Real Time qPCR (Q-PCR)
- Cell Viability Determination by MTT Colorimetric Assay
化学情報
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CAS 番号 293762-45-5
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性状 Solid
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分子量 340.38
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分子式 C20H16N6
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Color Brown to breen
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SMILES
NC1=CC=C2C(N=C(C3=CC=C4C(N=C(C5=CC=C(N)C=C5)N4)=C3)N2)=C1
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (3)
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Journal Impact Factor
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Most Recent
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Int Immunopharmacol
Ro 90-7501 suppresses colon cancer progression by inducing immunogenic cell death and promoting RIG-1-mediated autophagy. [Abstract]2024 Nov 15;143(Pt 3):113631. PMID: 39549547
Ro 90-7501 purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Nov 15;143(Pt 3):113631. [Abstract]
The effect of Ro 90-7501 (0, 10, 50, 100, 150, 200 μM) on the viability of HCT8 and SW480 cells.
Ro 90-7501 purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Nov 15;143(Pt 3):113631. [Abstract]
The impact of Ro 90-7501 (0, 10, 100, 200 μM) on the proliferation of HCT8 and SW480 cells.
Ro 90-7501 purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Nov 15;143(Pt 3):113631. [Abstract]
The influence of Ro 90-7501 (0, 10, 100, 200 μM) on the migration of HCT8 and SW480 cells.
Ro 90-7501 purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Nov 15;143(Pt 3):113631. [Abstract]
Ro 90-7501(0, 10, 100, 200 μM) promotes apoptosis in HCT8 and SW480 cells.
Ro 90-7501 purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Nov 15;143(Pt 3):113631. [Abstract]
The effect of Ro 90-7501 (0, 10, 100, 200 μM) on the expression of apoptosis-associated proteins.
Ro 90-7501 purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Nov 15;143(Pt 3):113631. [Abstract]
IHC staining to detect the expression of Ki-67 in implanted tumors in mice treated with Ro 90-7501 (10 mg/kg, i.p.).
Ro 90-7501 purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Nov 15;143(Pt 3):113631. [Abstract]
The effect of Ro 90-7501 (10 mg/kg, i.p.) on the volume and weight of implanted tumors in mice.
Ro 90-7501 purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 Nov 15;143(Pt 3):113631. [Abstract]
H&E staining to observe morphological changes in the heart, liver, spleen, lung, and kidney of mice treated with Ro 90-7501 (10 mg/kg, i.p.).
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Int Immunopharmacol
Inhibitors of APE1 redox and ATM synergistically sensitize osteosarcoma cells to ionizing radiation by inducing ferroptosis. [Abstract]2024 Sep 30:139:112672. PMID: 39032469 -
Int J Biochem Cell Biol
Modified 5-aminolevulinic acid photodynamic therapy (M-PDT) inhibits cutaneous squamous cell carcinoma cell proliferation via targeting PP2A/PP5-mediated MAPK signaling pathway. [Abstract]2021 Aug:137:106036. PMID: 34217813
溶剤 & 溶解度
体外:
DMSO : 41.67 mg/mL (122.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.08 mg/mL (6.11 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.11 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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データシート (274 KB)
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- Español - ES (394 KB)
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- Korean - KR (394 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Bohrmann B, et al. Self-assembly of beta-amyloid 42 is retarded by small molecular ligands at the stage of structural intermediates. J Struct Biol. 2000 Jun;130(2-3):232-46. [Content Brief]
[2]. Tamari K, et al. Ro 90-7501 Is a Novel Radiosensitizer for Cervical Cancer Cells that Inhibits ATM Phosphorylation. Anticancer Res. 2019 Sep;39(9):4805-4810. [Content Brief]
[3]. Guo F, et al. RO 90-7501 enhances TLR3 and RLR agonist induced antiviral response. PLoS One. 2012;7(10):e42583. [Content Brief]
[4]. Hong TJ, et al. Ro 90-7501 inhibits PP5 through a novel, TPR-dependent mechanism. Biochem Biophys Res Commun. 2017 Jan 8;482(2):215-220. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9379 mL | 14.6895 mL | 29.3789 mL | 73.4473 mL |
| 5 mM | 0.5876 mL | 2.9379 mL | 5.8758 mL | 14.6895 mL | |
| 10 mM | 0.2938 mL | 1.4689 mL | 2.9379 mL | 7.3447 mL | |
| 15 mM | 0.1959 mL | 0.9793 mL | 1.9586 mL | 4.8965 mL | |
| 20 mM | 0.1469 mL | 0.7345 mL | 1.4689 mL | 3.6724 mL | |
| 25 mM | 0.1175 mL | 0.5876 mL | 1.1752 mL | 2.9379 mL | |
| 30 mM | 0.0979 mL | 0.4896 mL | 0.9793 mL | 2.4482 mL | |
| 40 mM | 0.0734 mL | 0.3672 mL | 0.7345 mL | 1.8362 mL | |
| 50 mM | 0.0588 mL | 0.2938 mL | 0.5876 mL | 1.4689 mL | |
| 60 mM | 0.0490 mL | 0.2448 mL | 0.4896 mL | 1.2241 mL | |
| 80 mM | 0.0367 mL | 0.1836 mL | 0.3672 mL | 0.9181 mL | |
| 100 mM | 0.0294 mL | 0.1469 mL | 0.2938 mL | 0.7345 mL |
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