製品番号: HY-B1371

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(別名: Spiperone; Spiroperidol)

3 Cited Publications

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Based on 3 publication(s) in Google Scholar

Spiperone (Spiroperidol) is a potent dopamine D2, serotonin 5-HT_1A, and serotonin 5-HT_2A antagonist. Spiperone is also a labelled ligand for neuroleptic receptors. Spiperone enhances intracellular calcium level and inhibits the Wnt signaling pathway. Spiperone has the potential for the research of neurology diseases.

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純度: 99.00%
CAS 番号: 749-02-0
分子式: C₂₃H₂₆FN₃O₂
分子量:395.47

保管条件:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Spiperone 構造式

CAS 番号: 749-02-0

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Amount: USD 0.00

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性
- 製品説明
- IC₅₀ & Target
- Cellular Effect
- 体外実験
化学情報
Publications (3)
溶剤 & 溶解度
- 体外
- 体内
純度とドキュメンテーション
参考文献
Help & FAQs
関連分類
Bioz

生物活性

製品説明

IC₅₀ & Target

5-HT_1A Receptor

5-HT_2A Receptor

Dopamine Receptor アイソフォーム固有の製品をすべて表示 :

5-HT Receptor アイソフォーム固有の製品をすべて表示 :

Wnt アイソフォーム固有の製品をすべて表示 :

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	0.25 nM Compound: Spiperone	Displacement of [3H]Spiperone from human recombinant dopamine D2S receptor expressed in CHO cells after 2 hrs Displacement of [3H]Spiperone from human recombinant dopamine D2S receptor expressed in CHO cells after 2 hrs	[PMID: 23403082]
CHO	IC₅₀	0.36 nM Compound: Spiperone	Displacement of [3H]Spiperone from human recombinant dopamine D3 receptor expressed in CHO cells after 2 hrs Displacement of [3H]Spiperone from human recombinant dopamine D3 receptor expressed in CHO cells after 2 hrs	[PMID: 23403082]
CHO-K1	IC₅₀	0.5 nM Compound: Spiperone	Displacement of [3H]Spiperone from human recombinant dopamine D4 receptor expressed in CHOK1 cells after 2 hrs Displacement of [3H]Spiperone from human recombinant dopamine D4 receptor expressed in CHOK1 cells after 2 hrs	[PMID: 23403082]
CHO-K1	IC₅₀	97 nM Compound: Spiperone	Antagonist activity at dopamine D3 receptor (unknown origin) expressed in CHOK1 cells coexpressing Galpha15 assessed as inhibition of agonist-induced response incubated for 60 mins in incubator followed by 15 mins at room temperature by FLIPR assay Antagonist activity at dopamine D3 receptor (unknown origin) expressed in CHOK1 cells coexpressing Galpha15 assessed as inhibition of agonist-induced response incubated for 60 mins in incubator followed by 15 mins at room temperature by FLIPR assay	[PMID: 23675993]
H4	EC₅₀	>50 μM Compound: 12f	Induction of autophagy in human H4 cells expressing LC3 using DAPI staining after 4 hrs by fluorescence microscopic analysis Induction of autophagy in human H4 cells expressing LC3 using DAPI staining after 4 hrs by fluorescence microscopic analysis	[PMID: 21126871]
HEK293	IC₅₀	1.27 nM Compound: Spiperone	Displacement of [3H]Spiprone from human dopamine D2 receptor expressed in HEK293 cells Displacement of [3H]Spiprone from human dopamine D2 receptor expressed in HEK293 cells	[PMID: 22748706]

体外実験

Spiperone (5 μM, 24 h) specifically inhibits Wnt signaling by targeting the Wnt/LRP complex in HEK293 cells^[4].
Spiperone (1-10 μM, 275-470 s) induces a rise in intracellular calcium levels in HEK293 cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

化学情報

CAS 番号 749-02-0
性状 Solid
分子量 395.47
分子式 C₂₃H₂₆FN₃O₂
Color Off-white to light yellow
SMILES

O=C1NCN(C2=CC=CC=C2)C13CCN(CCCC(C4=CC=C(F)C=C4)=O)CC3
別名

Spiperone; Spiroperidol
輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (3)

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Insect Biochem Mol Biol

Molecular and pharmacological characterization of the dopamine receptors in the oriental fruit fly, Bactrocera dorsalis. [Abstract]

2025 May:180:104312. PMID: 40245998
J Ocul Pharmacol Ther

Survey of Dopamine Receptor D2 Antagonists as Retinal Antifibrotics. [Abstract]

2024 Oct;40(8):536-542. PMID: 39206555
bioRxiv

2026 Apr 1.

溶剤 & 溶解度

体外:

DMSO : 33.33 mg/mL (84.28 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5286 mL

12.6432 mL

25.2864 mL

5 mM

0.5057 mL

2.5286 mL

5.0573 mL

10 mM

0.2529 mL

1.2643 mL

2.5286 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 4.55 mg/mL (11.51 mM); Clear solution

This protocol yields a clear solution of ≥ 4.55 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (45.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

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COA (2659 KB)
元素分析報告書 (2659 KB)
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OR (2659 KB)
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取扱説明書 (2659 KB)

参考文献

[1]. Metwally KA, et al. Spiperone: influence of spiro ring substituents on 5-HT2A serotonin receptor binding. J Med Chem. 1998;41(25):5084-5093. [Content Brief]

[2]. Palacios JM, et al. [3H]Spiperone binding sites in brain: autoradiographic localization of multiple receptors. Brain Res. 1981 Jun 1;213(2):277-89. [Content Brief]

[3]. Leysen JE, et al. Spiperone: a ligand of choice for neuroleptic receptors. 1. Kinetics and characteristics of in vitro binding. Biochem Pharmacol. 1978 Feb 1;27(3):307-16. [Content Brief]

[4]. Lu D, et al. Spiperone enhances intracellular calcium level and inhibits the Wnt signaling pathway. BMC Pharmacol. 2009 Nov 30;9:13. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.5286 mL	12.6432 mL	25.2864 mL	63.2159 mL
	5 mM	0.5057 mL	2.5286 mL	5.0573 mL	12.6432 mL
	10 mM	0.2529 mL	1.2643 mL	2.5286 mL	6.3216 mL
	15 mM	0.1686 mL	0.8429 mL	1.6858 mL	4.2144 mL
	20 mM	0.1264 mL	0.6322 mL	1.2643 mL	3.1608 mL
	25 mM	0.1011 mL	0.5057 mL	1.0115 mL	2.5286 mL
	30 mM	0.0843 mL	0.4214 mL	0.8429 mL	2.1072 mL
	40 mM	0.0632 mL	0.3161 mL	0.6322 mL	1.5804 mL
	50 mM	0.0506 mL	0.2529 mL	0.5057 mL	1.2643 mL
	60 mM	0.0421 mL	0.2107 mL	0.4214 mL	1.0536 mL
	80 mM	0.0316 mL	0.1580 mL	0.3161 mL	0.7902 mL

Keywords

Spiperone

3 Cited Publications

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MedChemExpress（MCE）の使用を引用している文献 Spiperone

Dopamine Receptor アイソフォーム固有の製品をすべて表示

5-HT Receptor アイソフォーム固有の製品をすべて表示

Wnt アイソフォーム固有の製品をすべて表示

生物活性

化学情報

Publications (3)

溶剤 & 溶解度

純度とドキュメンテーション

参考文献

Help & FAQs

Complete Stock Solution Preparation Table

Spiperone 関連分類

HY-B1371 関連分類