2. Immunology/Inflammation Apoptosis
  3. SphK Apoptosis
  4. K145

K145 is a selective, substrate-competitive and orally active SphK2 inhibitor with an IC50 of 4.3 µM and a Ki of 6.4 µM. K145 is inactive against SphK1 and other protein kinases. K145 induces cell apoptosis and has potently antitumor activity.

For research use only. We do not sell to patients.

CAS No. : 1309444-75-4

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Other In-stock Forms of K145:

Other Forms of K145:

K145 Related Antibodies

Top Publications Citing Use of Products

    K145 purchased from MedChemExpress. Usage Cited in: Am J Cancer Res. 2019 Mar 1;9(3):546-561.  [Abstract]

    Western immunoblotting analysis of NOXA protein levels in RBE and HCCC9810 cells treated with different concentrations of K145 for 24 h. Data shown represents 2 independent experiments.

    K145 purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2017 Nov 4;493(1):286-290.  [Abstract]

    Inhibition of pAkt by API-2 effectively prevents K145 induced increasing phosphorylation of FoxO1 in response to insulin. API-2 also significantly reverses K145 suppressed PEPCK and G6Pase mRNA and protein expression.

    View All SphK Isoform Specific Products:

    View All Isoforms
    SphK1 SphK2

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    K145 is a selective, substrate-competitive and orally active SphK2 inhibitor with an IC50 of 4.3 µM and a Ki of 6.4 µM. K145 is inactive against SphK1 and other protein kinases. K145 induces cell apoptosis and has potently antitumor activity[1].

    IC50 & Target

    IC50: 4.3 µM (SphK2)[1]
    Ki: 6.4 µM (SphK2)[1]
    In Vitro

    K145 (0-10 µM; 24-72 hours; U937 cells) treatment significantly inhibits the growth of U937 cells in a concentration-dependent manner[1].
    K145 (10 µM; 24 hours; U937 cells) treatment significantly induces apoptosis in U937 cells[1].
    K145 (4-8 µM; 3 hours; U937 cells) treatment decreases the phosphorylation of ERK and Akt[1].
    Treatment with K145 (10 µM) causes a decrease of total cellular S1P without significant effects on ceramide levels[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    K145 Related Antibodies

    Cell Viability Assay[1]

    Cell Line: U937 cells
    Concentration: 0 µM, 4 µM, 6 µM, 8 µM, 10 µM
    Incubation Time: 24 hours, 48 hours, 72 hours
    Result: Significantly inhibited the growth of U937 cells in a concentration-dependent manner.

    Apoptosis Analysis[1]

    Cell Line: U937 cells
    Concentration: 10 µM
    Incubation Time: 24 hours
    Result: Significantly induced apoptosis in U937 cells.

    Western Blot Analysis[1]

    Cell Line: U937 cells
    Concentration: 4 µM, 8 µM
    Incubation Time: 3 hours
    Result: Phosphorylated ERK and Akt were decreased.
    In Vivo

    K145 (50 mg/kg; oral gavage; daily; for 15 days; BALB/c-nu mice) treatment significantly inhibits the growth of U937 tumors in nude mice[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: BALB/c-nu mice injected with U937 cells[1]
    Dosage: 50 mg/kg
    Administration: Oral gavage; daily; for 15 days
    Result: Oral gavage; daily; for 15 daysInhibited the growth of U937 tumors at 50 mg/kg dose and no apparent toxicity was observed.
    Molecular Weight

    348.46

    Formula

    C18H24N2O3S
    CAS No.
    SMILES

    O=C(N(CCN)C/1=O)SC1=C/CCC2=CC=C(OCCCC)C=C2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation
    References
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    K145 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    K1451309444-75-4K 145K-145SphKApoptosisSphingosine kinaseSphK2S1PERKAktapoptoticsubstrate-competitivesphingosineantitumororalanti-proliferativeInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    K145
    Cat. No.:
    HY-15779
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.