Cat. No.: HY-N0472

Geraniin

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

Geraniin is a TNF-α releasing inhibitor with numerous activities including anticancer, anti-inflammatory, and anti-hyperglycemic activities, with an IC₅₀ of 43 μM.

For research use only. We do not sell to patients.

Purity: 99.82%
CAS No.: 60976-49-0
Formula: C₄₁H₂₈O₂₇
Molecular Weight:952.64

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Geraniin Chemical Structure

CAS No.: 60976-49-0

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Geraniin is a TNF-α releasing inhibitor with numerous activities including anticancer, anti-inflammatory, and anti-hyperglycemic activities, with an IC₅₀ of 43 μM.

IC₅₀ & Target

IC50: 43 μM (TNF-α)^[1].

All TNF Receptor Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	ED₅₀	>10 μg/mL Compound: 2	Cytotoxicity against human A549 cells by tetrazolium salt-based colorimetric assay Cytotoxicity against human A549 cells by tetrazolium salt-based colorimetric assay	[PMID: 1431932]
HCT-8	ED₅₀	>10 μg/mL Compound: 2	Cytotoxicity against human HCT8 cells by tetrazolium salt-based colorimetric assay Cytotoxicity against human HCT8 cells by tetrazolium salt-based colorimetric assay	[PMID: 1431932]
Huh-7	CC₅₀	63.4 μM Compound: 22	Cytotoxicity against human Huh7.5 cells after 96 hrs by MTT assay Cytotoxicity against human Huh7.5 cells after 96 hrs by MTT assay	[PMID: 22196120]
Huh-7	EC₅₀	33.19 μM Compound: 22	Antiviral activity against Hepatitis C virus infected in human Huh7.5 cells assessed as inhibition of viral replication after 96 hrs by quantitative RT-PCR analysis Antiviral activity against Hepatitis C virus infected in human Huh7.5 cells assessed as inhibition of viral replication after 96 hrs by quantitative RT-PCR analysis	[PMID: 22196120]
KB	ED₅₀	>10 μg/mL Compound: 2	Cytotoxicity against human KB cells by tetrazolium salt-based colorimetric assay Cytotoxicity against human KB cells by tetrazolium salt-based colorimetric assay	[PMID: 1431932]
RD	IC₅₀	10 μg/mL Compound: Geraniin	Antiviral activity against C4 type Human enterovirus 71 FY0805 infected in human RD cells assessed as inhibition of viral replication by plaque reduction assay Antiviral activity against C4 type Human enterovirus 71 FY0805 infected in human RD cells assessed as inhibition of viral replication by plaque reduction assay	[PMID: 22342145]
TE-671	ED₅₀	>10 μg/mL Compound: 2	Cytotoxicity against human TE671 cells by tetrazolium salt-based colorimetric assay Cytotoxicity against human TE671 cells by tetrazolium salt-based colorimetric assay	[PMID: 1431932]

In Vitro

The IC₅₀ value of TNF-α release inhibition is 43 μM for Geraniin^[1]. Geraniin has long been used as a medicinal herb and possesses numerous activities including anticancer, anti-inflammatory, and anti-hyperglycemic activities. Geraniin significantly decreases the viability of OVCAR3 and SKOV3 cells in a concentration-dependent fashion. The IC₅₀ value for Geraniin treatment is 34.5±2.8 μM in OVCAR3 cells and 23.6±1.9 μM in SKOV3 cells. However, Geraniin up to the maximal concentration used (80 μM) has no significant impact on the viability of normal human ovarian surface epithelial cells. Treatment with 10 and 40 μM of Geraniin for 48 h causes a significant increase in apoptosis (16.8±1.2% and 22.6±1.4%, respectively), compared with control OVCAR3 cells (3.9±1.1%). Similar results are observed in SKOV3 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Treatment with Geraniin prior to application of okadaic acid delays development of tumors compared with control group, reduces the percentage of tumor bearing mice from 80.0% to 40.0%, and reduces the average numbers of tumor per mouse from 3.8 to 1.1 in week 20. It is also showed that oral administration of Geraniin to rats (50 mg/kg/d or 100 mg/kg/d) inhibit the elevation of serum total cholesterol, lipid peroxide, free fatty acid, triglyceride, glutamic oxaloacetic transaminase and glutamic pyruvic transaminase induced by treatment with peroxidized oil^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 60976-49-0
Appearance Solid
Molecular Weight 952.64
Formula C₄₁H₂₈O₂₇
Color Off-white to yellow
SMILES

O=C(OC[C@H](O[C@@H](OC(C1=CC(O)=C(O)C(O)=C1)=O)[C@H]2OC3=O)[C@H](OC(C(C4C5=C3C=C(O)C(O)=C5OC6(O)C4(O)O)=CC6=O)=O)[C@H]2O7)C8=CC(O)=C(O)C(O)=C8C9=C(O)C(O)=C(O)C=C9C7=O
Structure Classification
- Phenylpropanoids
- Other Phenylpropanoids
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (3)

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Journal Impact Factor

J Transl Med

Delineation of colorectal cancer ligand-receptor interactions and their roles in the tumor microenvironment and prognosis. [Abstract]

2021 Dec 7;19(1):497. PMID: 34876143
J Cell Mol Med

Geraniin Alleviates Mouse Laser-Induced Choroidal Neovascularisation by Inhibiting Choroidal Endothelial Cell ACE2/Ang-(1-7)/MasR/IL-10 Pathway. [Abstract]

2024 Dec;28(23):e70228. PMID: 39622780
J Chromatogr A

Screening and identification of acetylcholinesterase inhibitors from Terminalia chebula fruits by immobilized enzyme on cellulose filter paper coupled with ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry and molecular docking. [Abstract]

2022 Jan 25:1663:462784. PMID: 34974370

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (104.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.0497 mL

5.2486 mL

10.4971 mL

5 mM

0.2099 mL

1.0497 mL

2.0994 mL

10 mM

0.1050 mL

0.5249 mL

1.0497 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (2.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (2.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (2.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Cell Assay^[2]

Human ovarian cancer cell lines OVCAR3 and SKOV3 are used. Cells are exposed to different concentrations (5, 10, 20, 40, and 80 μM) of Geraniin for 48 h and examined for viability, apoptosis, and gene expression. The concentration range is selected based on previous studies^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Okabe S, et al. New TNF-alpha releasing inhibitors, geraniin and corilagin, in leaves of Acer nikoense, Megusurino-ki. Biol Pharm Bull. 2001 Oct;24(10):1145-8. [Content Brief]

[2]. Wang X, et al. Geraniin suppresses ovarian cancer growth through inhibition of NF-κB activation and downregulation of Mcl-1 expression. J Biochem Mol Toxicol. 2017 Sep;31(9). [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.0497 mL	5.2486 mL	10.4971 mL	26.2429 mL
	5 mM	0.2099 mL	1.0497 mL	2.0994 mL	5.2486 mL
	10 mM	0.1050 mL	0.5249 mL	1.0497 mL	2.6243 mL
	15 mM	0.0700 mL	0.3499 mL	0.6998 mL	1.7495 mL
	20 mM	0.0525 mL	0.2624 mL	0.5249 mL	1.3121 mL
	25 mM	0.0420 mL	0.2099 mL	0.4199 mL	1.0497 mL
	30 mM	0.0350 mL	0.1750 mL	0.3499 mL	0.8748 mL
	40 mM	0.0262 mL	0.1312 mL	0.2624 mL	0.6561 mL
	50 mM	0.0210 mL	0.1050 mL	0.2099 mL	0.5249 mL
	60 mM	0.0175 mL	0.0875 mL	0.1750 mL	0.4374 mL
	80 mM	0.0131 mL	0.0656 mL	0.1312 mL	0.3280 mL
	100 mM	0.0105 mL	0.0525 mL	0.1050 mL	0.2624 mL

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Geraniin

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Publications Citing Use of MedChemExpress (MCE) Geraniin

All TNF Receptor Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Geraniin Related Classifications

HY-N0472 Related Classifications