Cat. No.: HY-110127

NU9056

7 Cited Publications

고객리뷰

Based on 7 publication(s) in Google Scholar

NU9056 is a potent and selective Tip60 (KAT5) histone acetyltransferase inhibitor with an of 2 µM. NU9056 shows >16-fold selectivity for Tip60 over PCAF, p300 and GCN5. NU9056 induces apoptosis of prostate cancer cells.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity: 96.62%
CAS No.: 1450644-28-6
화학식: C₆H₄N₂S₄
분자량:232.37

보관:

Pure form -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

NU9056 Chemical Structure

CAS No.: 1450644-28-6

사이즈

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- In Vitro
- In Vivo
Chemical Information
Publications (7)
용액&용해도
- In Vitro
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

NU9056 is a potent and selective Tip60 (KAT5) histone acetyltransferase inhibitor with an ^[1]^[2] of 2 µM. NU9056 shows >16-fold selectivity for Tip60 over PCAF, p300 and GCN5. NU9056 induces apoptosis of prostate cancer cells^[1].

All Histone Acetyltransferase Isoforms :

In Vitro

NU9056 (17-36 μM; 24-96 hours) results in both caspase 3 and caspase 9 activation in a time- and concentration-dependent manner^[1].
? NU9056 (2.5-40 μM; 2 hours) treatment results in decreased levels of acetylated histone H4K16, H3K14 and H4K8, targets for Tip60-mediated acetylation^[1].
? NU9056 treatment also decreases androgen receptor, prostate specific antigen, p53 and p21 protein levels^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

LNCaP cells
Concentration:

17 µM, 24 µM, 36 µM
Incubation Time:

24 hours, 48 hours, 72 hours, 96 hours
Result:

Induced apoptosis via activation of caspase 3 and caspase 9 in a concentration- and time-dependent manner.

Western Blot Analysis^[1]

Cell Line:

LNCaP cells
Concentration:

2.5 µM, 5 µM, 10 µM, 20 µM, 40 µM
Incubation Time:

2 hours
Result:

Resulted in decreased levels of acetylated histone H4K16, H3K14 and H4K8, targets for Tip60-mediated acetylation.

In Vivo

The mice are injected with Nu9056 (2 μg/g) and the hippocampus is collected 1 h later. Tip60 inhibition reduces H2A.Z binding at the -1 nucleosome of Arc, and the +1 nucleosome of Arc and Syp. Additionally, Nu9056 increases acetylation at the -1 nucleosome of Fos, Tacstd2, and Gria4, and the +1 nucleosome of Gria4^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 1450644-28-6
Appearance Liquid (Density: 1.60±0.1 g/cm³)
분자량 232.37
화학식 C₆H₄N₂S₄
Color Yellow to brown
SMILES

C1(SSC2=CC=NS2)=CC=NS1
선적

Room temperature in continental US; may vary elsewhere.

보관

Pure form	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (7)

All RT-PCRBio/Physico-chemical AssayWB

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Journal Impact Factor

Nature

Acetyl-methyllysine marks chromatin at active transcription start sites. [Abstract]

2023 Oct;622(7981):173-179. PMID: 37731000

NU9056 purchased from MedChemExpress. Usage Cited in: Nature. 2023 Oct;622(7981):173-179. [Abstract]

Representative western blots of two biological replicates of HEK293T histones treated with A-485 and NU9056 (10 μM for 24 hours).
Cell Host Microbe

Uric acid promotes dietary lipid absorption through microbiome and metabolomic remodeling via a liver-gut endocrine axis. [Abstract]

2026 Jun 3:S1931-3128(26)00181-2. PMID: 42235504
Adv Sci (Weinh)

Lactylated Apolipoprotein C-II Induces Immunotherapy Resistance by Promoting Extracellular Lipolysis. [Abstract]

2024 Oct;11(38):e2406333. PMID: 38981044

NU9056 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Oct;11(38):e2406333. [Abstract]

H1299 cells were treated with lactate, A‐485, KAT8‐IN‐1, NU9056 (2 µM), Butyrolactone 3 (MB‐3), or Gln‐AMS for 14 h. Quantitative analysis of extracellular FFAs was conducted post‐treatment.
Cancer Lett

Acetylation-dependent deubiquitinase USP26 stabilizes BAG3 to promote breast cancer progression. [Abstract]

2024 Aug 10:597:217005. PMID: 38880224

NU9056 purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2024 Aug 10:597:217005. [Abstract]

Treatment with the Tip60 inhibitor NU9056 (20 μM for 18 h) reduced USP26 acetylation. Input and anti-HA immunoprecipitates from HEK293T cells transfected with or without Flag-Tip60 were analyzed by WB.
Sens Actuators B Chem

2024 Aug 1, 412, 135827.

NU9056 purchased from MedChemExpress. Usage Cited in: Sens Actuators B Chem. 2024 Aug 1, 412, 135827.

The effects of adding NU9056 (0-500 μM) to the Tip60 reaction system were analyzed for fluorescence readout and for UV–vis absorbance readout.

NU9056 purchased from MedChemExpress. Usage Cited in: Sens Actuators B Chem. 2024 Aug 1, 412, 135827.

Kinetic plots displaying the corresponding relative activity based on fluorescence readout and UV–vis absorbance readout are presented, plotted against the logarithm of NU9056 (0-50 μM) concentrations.
Mol Cell Proteomics

P300 interacted with N-Myc and regulated its protein stability via altering its posttranslational modifications in neuroblastoma. [Abstract]

2023 Mar;22(3):100504. PMID: 36708875
Fish Shellfish Immunol

Grass carp (Ctenopharyngodon idella) Kat5 mediates P53 acetylation to induce apoptosis and promotes GCRV replication. [Abstract]

2026 Jan:168:110946. PMID: 41109541

NU9056 purchased from MedChemExpress. Usage Cited in: Fish Shellfish Immunol. 2026 Jan:168:110946. [Abstract]

The healthy grass carp were separately intraperitoneally injected with 21 μl of NU9056 at a concentration of 20 mM or the same amount of DMSO. After 4 days, the tissues of intestines, liver, spleen, and kidney were separately extracted. The mRNA levels of bax, bcl2, and caspase3 were assessed by qRT-PCR.

NU9056 purchased from MedChemExpress. Usage Cited in: Fish Shellfish Immunol. 2026 Jan:168:110946. [Abstract]

The healthy grass carp were separately intraperitoneally injected with 21 μl of NU9056 at a concentration of 20 mM or the same amount of DMSO. After 4 days, the tissues of intestines, liver, spleen, and kidney were separately extracted. Caspase3 protein levels were analyzed by Western-blot.

용액&용해도

In Vitro:

DMSO : ≥ 125 mg/mL (537.94 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.3035 mL

21.5174 mL

43.0348 mL

5 mM

0.8607 mL

4.3035 mL

8.6070 mL

10 mM

0.4303 mL

2.1517 mL

4.3035 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Kelly Coffey, et al. Characterisation of a Tip60 specific inhibitor, NU9056, in prostate cancer. PLoS One. 2012;7(10):e45539. [Content Brief]

[2]. Klotilda Narkaj, et al. Blocking H2A.Z Incorporation via Tip60 Inhibition Promotes Systems Consolidation of Fear Memory in Mice. eNeuro. 2018 Nov 8;5(5):ENEURO.0378-18.2018. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.3035 mL	21.5174 mL	43.0348 mL	107.5870 mL
	5 mM	0.8607 mL	4.3035 mL	8.6070 mL	21.5174 mL
	10 mM	0.4303 mL	2.1517 mL	4.3035 mL	10.7587 mL
	15 mM	0.2869 mL	1.4345 mL	2.8690 mL	7.1725 mL
	20 mM	0.2152 mL	1.0759 mL	2.1517 mL	5.3794 mL
	25 mM	0.1721 mL	0.8607 mL	1.7214 mL	4.3035 mL
	30 mM	0.1434 mL	0.7172 mL	1.4345 mL	3.5862 mL
	40 mM	0.1076 mL	0.5379 mL	1.0759 mL	2.6897 mL
	50 mM	0.0861 mL	0.4303 mL	0.8607 mL	2.1517 mL
	60 mM	0.0717 mL	0.3586 mL	0.7172 mL	1.7931 mL
	80 mM	0.0538 mL	0.2690 mL	0.5379 mL	1.3448 mL
	100 mM	0.0430 mL	0.2152 mL	0.4303 mL	1.0759 mL

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NU9056

Apoptosis Compound Library

Epigenetics Compound Library

Histone Modification Research Compound Library

Anti-Cancer Compound Library

Anti-Cardiovascular Disease Compound Library

Anti-Prostate Cancer Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Bioactive Compound Library Max

Epigenetics Compound Library Mini

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) NU9056

All Histone Acetyltransferase Isoforms

Biological Activity

Chemical Information

Publications (7)

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

NU9056

Apoptosis Compound Library

Epigenetics Compound Library

Histone Modification Research Compound Library

Anti-Cancer Compound Library

Anti-Cardiovascular Disease Compound Library

Anti-Prostate Cancer Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Bioactive Compound Library Max

Epigenetics Compound Library Mini

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) NU9056

All Histone Acetyltransferase Isoforms

Biological Activity

Chemical Information

Publications (7)

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

NU9056 Related Classifications

HY-110127 Related Classifications