PMSF
Based on 161 publication(s) in Google Scholar
PMSF (Phenylmethylsulfonyl fluoride) is a nonspecific and irreversible serine proteases inhibitor. PMSF inhibits some thiol proteases, non-protease enzymes, and acetylcholinesterase. PMSF can be used to prepare protein extracts from tissue and cell lysates.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity: 99.32%
- CAS No.: 329-98-6
- 화학식: C7H7FO2S
- 분자량:174.19
-
보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
용액&용해도
- Protocol
- 순도&문서
- References
-
Help & FAQs
-
Metabolism/Protease Compound Library
HY-L012
-
Covalent Screening Library
HY-L036
-
Angiogenesis-Related Compound Library
HY-L088
-
Human Metabolite Library
HY-L123
-
Coagulation and Anti-coagulation Compound Library
HY-L136
-
Protease Inhibitor Library
HY-L147
-
Highly Selective Inhibitors Library
HY-L158
-
Serine/Threonine Kinase Inhibitor Library
HY-L164
-
Multi-Target Compound Library
HY-L176
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Covalent Screening Library Plus
HY-L036P
-
Bioactive Compound Library
HY-L001
-
Protein Kinase Compound Library
HY-L196
-
High-Throughput Bioactive Compound Library
HY-L205
-
Mass Spectrometry Human Metabolite Library
HY-L215
Publications Citing Use of MedChemExpress (MCE) PMSF
More- Signal Transduct Target Ther. 2025 Jun 30;10(1):205. [Abstract]
- Cancer Cell. 2025 Jul 17:S1535-6108(25)00273-9. [Abstract]
- Cell. 2026 Jun 11;189(12):3553-3570.e30. [Abstract]
- Cell. 2026 Feb 5;189(3):853-871.e31. [Abstract]
- Mol Cancer. 2026 May 13.
- Circulation. 2022 Mar;145(9):675-687. [Abstract]
- Mol Plant. 2025 Nov 17:S1674-2052(25)00394-6. [Abstract]
- Nat Metab. 2026 Jan;8(1):74-95. [Abstract]
- Nat Biomed Eng. 2025 Jun;9(6):952-966. [Abstract]
- Bioact Mater. 2025 Oct 29:56:468-482. [Abstract]
- Cancer Res. 2025 Feb 17;85(4):692-703. [Abstract]
- J Extracell Vesicles. 2025 Sep;14(9):e70162. [Abstract]
- J Extracell Vesicles. 2025 Jan;14(1):e70041. [Abstract]
- Bone Res. 2025 Mar 3;13(1):30. [Abstract]
- Autophagy. 2025 Jul 11:1-19. [Abstract]
- Autophagy. 2024 Oct;20(10):2221-2237. [Abstract]
- Autophagy. 2024 Nov;20(11):2556-2570. [Abstract]
- Autophagy. 2023 Jun;19(6):1745-1763. [Abstract]
- Autophagy. 2021 Oct;17(10):3030-3047. [Abstract]
- Nat Commun. 2026 Jun 15. [Abstract]
- Nat Commun. 2024 Dec 4;15(1):10543. [Abstract]
- J Adv Res. 2026 Mar 23:S2090-1232(26)00271-7. [Abstract]
- J Adv Res. 2024 Feb:56:125-136. [Abstract]
- Redox Biol. 2026 Jul:94:104146. [Abstract]
- Adv Sci (Weinh). 2026 May 26:e15158. [Abstract]
- Adv Sci (Weinh). 2026 Mar 12:e20455. [Abstract]
- MedComm (2020). 2025 Oct 30;6(11):e70438. [Abstract]
- Adv Sci (Weinh). 2025 Apr 25:e2501046. [Abstract]
- Adv Sci (Weinh). 2025 Mar 24:e2500021. [Abstract]
- Sci Adv. 2026 Jan 2;12(1):eady2539. [Abstract]
- Chem Eng J. 2024 Sep 20.
- Cell Death Dis. 2024 Oct 27;15(10):779. [Abstract]
- Cell Death Dis. 2024 Oct 21;15(10):764. [Abstract]
- Cell Death Dis. 2022 Dec 27;13(12):1075. [Abstract]
- Small. 2020 Dec 2;e2004879. [Abstract]
- Cell Commun Signal. 2025 Mar 29;23(1):156. [Abstract]
- Cell Commun Signal. 2023 Oct 5;21(1):273. [Abstract]
- Phytomedicine. 2022 Feb:96:153891. [Abstract]
- Cell Death Discov. 2026 Jan 9;12(1):11. [Abstract]
- J Transl Med. 2025 Nov 7;23(1):1243. [Abstract]
- Dev Cell. 2024 Jul 16:S1534-5807(24)00398-8. [Abstract]
- Dev Cell. 2024 May 17:S1534-5807(24)00296-X. [Abstract]
- Int J Biol Macromol. 2026 Jan;335(Pt 2):149182. [Abstract]
- Int J Biol Macromol. 2025 Dec;333(Pt 2):148692. [Abstract]
- Neural Regen Res. 2024 Dec 16. [Abstract]
- Int J Mol Med. 2020 Sep;46(3):1063-1073. [Abstract]
- Free Radic Biol Med. 2026 Jun 6:254:46-61. [Abstract]
- Free Radic Biol Med. 2025 Aug 21:240:426-438. [Abstract]
- Free Radic Biol Med. 2025 Oct:238:522-541. [Abstract]
- Free Radic Biol Med. 2025 Sep:237:88-100. [Abstract]
- Free Radic Biol Med. 2025 Apr 28:235:150-161. [Abstract]
- EMBO Mol Med. 2023 Oct 11;15(10):e16836. [Abstract]
- Br J Cancer. 2025 May 28. [Abstract]
- Cell Rep. 2025 Feb 5;44(2):115246. [Abstract]
- Aging Cell. 2024 Sep 17:e14336. [Abstract]
- Cell Prolif. 2021 Dec;54(12):e13155. [Abstract]
- Int J Oncol. 2026 May;68(5):58. [Abstract]
- Int J Oncol. 2025 Oct;67(4):85. [Abstract]
- J Med Chem. 2022 Mar 24;65(6):5057-5071. [Abstract]
- CNS Neurosci Ther. 2026 Apr;32(4):e70910. [Abstract]
- Ecotoxicol Environ Saf. 2025 Mar 15:293:118029. [Abstract]
- Biochem Pharmacol. 2026 Jun 25;251(Pt 2):118188. [Abstract]
- Biochem Pharmacol. 2026 Jun:248:117825. [Abstract]
- Cell Mol Life Sci. 2024 Sep 11;81(1):397. [Abstract]
- Biochem Pharmacol. 2024 Jul 4:116412. [Abstract]
- Food Funct. 2023 Nov 13;14(22):9999-10013. [Abstract]
- Drug Des Devel Ther. 2023 Aug 16:17:2383-2399. [Abstract]
- J Cell Biol. 2023 May 1;222(5):e202208159. [Abstract]
- Neurobiol Dis. 2026 Jun 15:224:107397. [Abstract]
- Cells. 2022 Nov 5;11(21):3505. [Abstract]
- ACS Biomater Sci Eng. 2021 Jul 12;7(7):3256-3268. [Abstract]
- EMBO Rep. 2021 May 5;22(5):e51660. [Abstract]
- J Inflamm (Lond). 2025 Jan 24;22(1):4. [Abstract]
- Mol Cancer Res. 2024 Jul 2;22(7):668-681. [Abstract]
- Mol Cancer Res. 2020 Feb;18(2):204-215. [Abstract]
- Respir Res. 2024 Aug 29;25(1):326. [Abstract]
- Pharmaceuticals (Basel). 2023 Nov 1;16(11):1545. [Abstract]
- Eur J Pharmacol. 2022 Jun 15;925:175002. [Abstract]
- Int Immunopharmacol. 2026 May 14:182:116851. [Abstract]
- Int Immunopharmacol. 2024 Jun 21:137:112498. [Abstract]
- Int J Mol Sci. 2024 Jul 7;25(13):7461. [Abstract]
- Int Immunopharmacol. 2019 Apr:69:143-149. [Abstract]
- Inflammation. 2024 Dec;47(6):2094-2107. [Abstract]
- Front Pharmacol. 2022 Mar 14;13:824579. [Abstract]
- Front Pharmacol. 2021 Nov 26;12:791644. [Abstract]
- Biogerontology. 2025 Dec 16;27(1):24. [Abstract]
- Front Cell Dev Biol. 2022 Apr 4;10:852244. [Abstract]
- Front Chem. 2021 Dec 3:9:783705. [Abstract]
- Toxicology. 2024 Aug 13:153924. [Abstract]
- Molecules. 2022 Oct 6;27(19):6637. [Abstract]
- Molecules. 2022 May 13;27(10):3121. [Abstract]
- Bioorg Chem. 2022 Mar:120:105605. [Abstract]
- Molecules. 2021 Sep 8;26(18):5462. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2025 Feb 26;1871(4):167748. [Abstract]
- Mol Med Rep. 2023 Feb;27(2):45. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2022 Sep 1;1868(9):166450. [Abstract]
- Sci Rep. 2025 Oct 24;15(1):37197. [Abstract]
- Cancer Sci. 2025 Sep 2. [Abstract]
- J Lipid Res. 2024 Aug 20:100626. [Abstract]
- Sci Rep. 2020 Aug 3;10(1):13063. [Abstract]
- J Extracell Biol. 2025 Jun 2;4(6):e70052. [Abstract]
- Cell Signal. 2025 Mar 29:111781. [Abstract]
- Cell Signal. 2025 Jan 21:127:111614. [Abstract]
- J Inflamm Res. 2026 Jan 20;19:1-13.
- iScience. 2026 Jun 1;29(6):116200. [Abstract]
- Metabolites. 2025 Dec 26;16(1):31. [Abstract]
- ACS Infect Dis. 2025 Dec 12;11(12):3420-3434. [Abstract]
- Biologics. 2024 May 23:18:129-142. [Abstract]
- Front Mol Biosci. 2021 May 21:8:682769. [Abstract]
- J Neurosci. 2025 Aug 6;45(32):e1995242025. [Abstract]
- Wound Repair Regen. 2022 May;30(3):376-396. [Abstract]
- J Pharmacol Exp Ther. 2019 Jul;370(1):62-71. [Abstract]
- Fish Shellfish Immunol. 2024 May:148:109515. [Abstract]
- Chem Res Toxicol. 2026 Jun 15;39(6):1088-1101. [Abstract]
- J Virol. 2026 Jun 23;100(6):e0018526. [Abstract]
- Front Biosci (Landmark Ed). 2024 Jul 26;29(7):274. [Abstract]
- BMC Cancer. 2022 Jul 10;22(1):751. [Abstract]
- J Chromatogr A. 2026 Feb 8:1768:466675. [Abstract]
- J Immunol. 2022 Nov 1;209(9):1796-1805. [Abstract]
- J Cell Physiol. 2019 Dec;234(12):22960-22971. [Abstract]
- Tumour Virus Res. 2026 Mar 26:21:200340. [Abstract]
- Viruses. 2025 Oct 13;17(10):1369. [Abstract]
- Exp Cell Res. 2024 Oct 1;442(2):114285. [Abstract]
- Exp Cell Res. 2024 Mar 15;436(2):113980. [Abstract]
- Exp Cell Res. 2023 Jul 15;428(2):113620. [Abstract]
- Bioorg Med Chem. 2023 Mar 15:82:117237. [Abstract]
- Exp Cell Res. 2022 Aug 15;417(2):113220. [Abstract]
- Front Oncol. 2024 Apr 3:14:1283164. [Abstract]
- J Leukoc Biol. 2022 Mar;111(3):641-653. [Abstract]
- Front Oncol. 2021 Mar 23:11:629916. [Abstract]
- Mol Biol Rep. 2024 Mar 14;51(1):421. [Abstract]
- Tissue Cell. 2025 Dec 3:99:103257. [Abstract]
- BMC Vet Res. 2022 Aug 11;18(1):307. [Abstract]
- PeerJ. 2023 Dec 12:11:e16463. [Abstract]
- J Assist Reprod Genet. 2025 Feb;42(2):665-678. [Abstract]
- Cancer Manag Res. 2026 Apr 1:18:594906. [Abstract]
- Biochem Biophys Res Commun. 2023 Aug 6:668:8-18. [Abstract]
- Biochem Biophys Res Commun. 2023 Feb 19:645:55-60. [Abstract]
- Cell Physiol Biochem. 2017;44(3):1051-1063. [Abstract]
- Exp Brain Res. 2025 Dec 16;244(1):13. [Abstract]
- Oncol Lett. 2020 Mar;19(3):2466-2474. [Abstract]
- J Obstet Gynaecol Res. 2025 Sep;51(9):e70069. [Abstract]
- Curr Eye Res. 2022 Mar;47(3):343-351. [Abstract]
- Cent Eur J Immunol. 2025;50(3):248-259. [Abstract]
- J Spinal Cord Med. 2026 Jun 16:1-12. [Abstract]
- Isr J Plant Sci. 2021 Mar 18.
- Comput Biol Med. 2025 Jul 5;196(Pt A):110721. [Abstract]
- SSRN. 2025 Jul 2.
- Heliyon. 2024 Sep 18;10(19):e38026. [Abstract]
- Pediatr Discov. 2024 Jul 9;2(4):e94. [Abstract]
- Heliyon. 2024 Aug 18;10(16):e36205. [Abstract]
- Heliyon. Heliyon. 2024 Jul 18;10(15):e34870. [Abstract]
- Research Square Preprint. 2023 Oct 17.
- SSRN. 2023 Jun 27.
- Braz J Biol. 2023 Jan 6:82:e266573. [Abstract]
- Research Square Print. December 5th, 2022.
- Research Square Preprint. 2021 Dec.
- Research Square Preprint. 2021 Nov.
- Research Square Preprint. 2021 Jul.
- Korean Journal of Medicinal Crop Science. 2021; 29(2):135.
- Heliyon. 2020 Aug;6(8):e04793. [Abstract]
All Cathepsin Isoforms
More
Biological Activity
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| Neutrophil | IC50 |
130.93 μM
Compound: PMSF
|
Antiinflammatory activity in neutrophil assessed as inhibition of fMLP induced elastase release
Antiinflammatory activity in neutrophil assessed as inhibition of fMLP induced elastase release
|
[PMID: 17197180] |
| Neutrophil | IC50 |
35.68 μM
Compound: phenylmethylsulfonyl fluoride
|
Antiinflammatory activity in human neutrophils assessed as inhibition of fMet-Leu-Phe/Cytochalasin B-induced elastase release at 10 ug/mL
Antiinflammatory activity in human neutrophils assessed as inhibition of fMet-Leu-Phe/Cytochalasin B-induced elastase release at 10 ug/mL
|
[PMID: 18163582] |
| Neutrophil | IC50 |
204.08 μM
Compound: phenylmethylsulfonyl fluoride
|
Antiinflammatory activity in human neutrophils assessed as inhibition of formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B-induced elastase release
Antiinflammatory activity in human neutrophils assessed as inhibition of formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B-induced elastase release
|
[PMID: 18211005] |
| Neutrophil | IC50 |
202.3 μM
Compound: phenylmethylsulfonyl fluoride
|
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/Cytochalasin B-induced elastase release
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/Cytochalasin B-induced elastase release
|
[PMID: 18817445] |
| Neutrophil | IC50 |
130.9 μM
Compound: PMSF
|
Antiinflammatory activity in fMLP/cytochalasin B-stimulated human neutrophils assessed as inhibition of elastase release after 5 mins
Antiinflammatory activity in fMLP/cytochalasin B-stimulated human neutrophils assessed as inhibition of elastase release after 5 mins
|
[PMID: 19110343] |
| Neutrophil | IC50 |
35.24 μg/mL
Compound: Phenylmethylsulfonyl fluoride
|
Antiinflammatory activity against human neutrophils assessed as inhibition of fMLP/CB-induced elastase release
Antiinflammatory activity against human neutrophils assessed as inhibition of fMLP/CB-induced elastase release
|
[PMID: 19128011] |
| Neutrophil | IC50 |
203.5 μM
Compound: phenylmethylsulfonyl fluoride
|
Antiinflammatory activity in human neutrophils assessed as inhibition of fMet-Leu-Phe/Cytochalasin B-induced elastase release treated 5 mins before fMet-Leu-Phe/Cytochalasin B challenge
Antiinflammatory activity in human neutrophils assessed as inhibition of fMet-Leu-Phe/Cytochalasin B-induced elastase release treated 5 mins before fMet-Leu-Phe/Cytochalasin B challenge
|
[PMID: 19203247] |
| Neutrophil | IC50 |
95 μM
Compound: PMSF
|
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/Cytochalasin B-induced elastase release
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/Cytochalasin B-induced elastase release
|
[PMID: 19583252] |
| Neutrophil | IC50 |
130.9 μM
Compound: PMSF
|
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/cytochalasin B-induced elastase release
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/cytochalasin B-induced elastase release
|
[PMID: 20036537] |
| Neutrophil | IC50 |
19.89 μg/mL
Compound: PMSF
|
Antiinflammatory activity in human neutrophils assessed as inhibition of FMLP/CB-induced elastase release
Antiinflammatory activity in human neutrophils assessed as inhibition of FMLP/CB-induced elastase release
|
[PMID: 21106454] |
| Neutrophil | IC50 |
95 μM
Compound: PMSF
|
Inhibition of fMLP/CB-activated human neutrophil degranulation assessed as inhibition of elastase release using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as a substrate after 5 mins
Inhibition of fMLP/CB-activated human neutrophil degranulation assessed as inhibition of elastase release using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as a substrate after 5 mins
|
[PMID: 21417387] |
| Neutrophil | IC50 |
95 μM
Compound: PMSF
|
Inhibition of FMLP/CB-stimulated elastase release in human neutrophils treated for 5 mins before FMLP/CB challenge using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as a substrate
Inhibition of FMLP/CB-stimulated elastase release in human neutrophils treated for 5 mins before FMLP/CB challenge using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as a substrate
|
[PMID: 21441032] |
| Neutrophil | IC50 |
22.8 μg/mL
Compound: PMSF
|
Antiinflammatory activity in human neutrophils assessed as inhibition of FMLP/CB-induced elastase release incubated for 5 mins prior to FMLP/CB-challenge by spectrophotometry
Antiinflammatory activity in human neutrophils assessed as inhibition of FMLP/CB-induced elastase release incubated for 5 mins prior to FMLP/CB-challenge by spectrophotometry
|
[PMID: 22413887] |
| Neutrophil | IC50 |
131 μg/mL
Compound: PMSF
|
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release using MeO-Suc-Ala-Ala-Pro-Val-MC as substrate by spectrophotometry
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release using MeO-Suc-Ala-Ala-Pro-Val-MC as substrate by spectrophotometry
|
[PMID: 22595179] |
| Neutrophil | IC50 |
95 μM
Compound: Phenylmethylsulfonyl fluoride
|
Inhibition of fMLF/CB-induced human Neutrophil activation assessed as reduction in superoxide anion
Inhibition of fMLF/CB-induced human Neutrophil activation assessed as reduction in superoxide anion
|
[PMID: 37683361] |
PMSF (2 mM) inhibits carbachol-stimulated inositol phosphate accumulation in the presence of Li+ by only 15%-19%. PMSF inhibition of phosphoinositide turnover is due to one or more steps following phosphoinositide breakdown[1]. PMSF inhibits the acylation of the inositol residue of GPI intermediates in bloodstream-form T. brucei. PMSF inhibits the formation of glycolipid C but does not inhibit fatty acid remodeling in vitro. PMSF inhibits GPI acylation and ethanolamine phosphate addition in procyclic trypanosomes but not in Hela cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 329-98-6
-
Appearance Solid
-
분자량 174.19
-
화학식 C7H7FO2S
-
Color White to off-white
-
SMILES
O=S(CC1=CC=CC=C1)(F)=O
-
Synonyms
Phenylmethylsulfonyl fluoride; Benzylsulfonyl fluoride
-
선적
Room temperature in continental US; may vary elsewhere.
-
보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (161)
-
Journal Impact Factor
-
Most Recent
-
Signal Transduct Target Ther
Serine/threonine/tyrosine kinase 1 drives pancreatic carcinogenesis via GSK3β sequestration-mediated Wnt/β-catenin pathway hyperactivation. [Abstract]2025 Jun 30;10(1):205. PMID: 40588478 -
Cancer Cell
2025 Jul 17:S1535-6108(25)00273-9. PMID: 40712567 -
Cell
2026 Jun 11;189(12):3553-3570.e30. PMID: 42049018 -
Cell
Peripheral cancer attenuates amyloid pathology in Alzheimer's disease via cystatin-c activation of TREM2. [Abstract]2026 Feb 5;189(3):853-871.e31. PMID: 41576952 -
-
Circulation
Ablation of Plasma Prekallikrein Decreases Low-Density Lipoprotein Cholesterol by Stabilizing Low-Density Lipoprotein Receptor and Protects Against Atherosclerosis. [Abstract]2022 Mar;145(9):675-687. PMID: 35189703 -
Mol Plant
Pathogen-induced H2O2 triggers phosphorylation of the RNA-binding protein RRM2R to suppress OsMAPKKK18 intron retention for orchestrating rice immunity and growth. [Abstract]2025 Nov 17:S1674-2052(25)00394-6. PMID: 41254943 -
Nat Metab
2026 Jan;8(1):74-95. PMID: 41507665 -
Nat Biomed Eng
Concurrent intratumoural Treg cell depletion and CD8+ T cell expansion via a cleavable anti-4-1BB-interleukin-15 fusion protein. [Abstract]2025 Jun;9(6):952-966. PMID: 39623095 -
Bioact Mater
Study on the mechanism of hUCMSCs-derived 3D exosome combined with HAMA microneedles in the treatment of deep second-degree burns. [Abstract]2025 Oct 29:56:468-482. PMID: 41234297 -
Cancer Res
Adenosine Uptake through the Nucleoside Transporter ENT1 Suppresses Antitumor Immunity and T-cell Pyrimidine Synthesis. [Abstract]2025 Feb 17;85(4):692-703. PMID: 39652568 -
J Extracell Vesicles
Targeted Blockage of Pathological Extracellular Vesicles and Particles From Fibroblast-Like Synoviocytes for Osteoarthritis Relief: Proteomic Analysis and Cellular Effect. [Abstract]2025 Sep;14(9):e70162. PMID: 40919882 -
J Extracell Vesicles
Extracellular Vesicles From Bone Marrow-Derived Macrophages Enriched in ARG1 Enhance Microglial Phagocytosis and Haematoma Clearance Following Intracerebral Haemorrhage. [Abstract]2025 Jan;14(1):e70041. PMID: 39868438 -
Bone Res
Enhanced SIRT3 expression restores mitochondrial quality control mechanism to reverse osteogenic impairment in type 2 diabetes mellitus. [Abstract]2025 Mar 3;13(1):30. PMID: 40025004 -
Autophagy
2025 Jul 11:1-19. PMID: 40568837 -
Autophagy
PLK2-mediated phosphorylation of SQSTM1 S349 promotes aggregation of polyubiquitinated proteins upon proteasomal dysfunction. [Abstract]2024 Oct;20(10):2221-2237. PMID: 39316746 -
Autophagy
Signal-sustained imaging of mitophagy with an Enzyme-Activatable Metabolic Lipid-Labeling Probe. [Abstract]2024 Nov;20(11):2556-2570. PMID: 38873937 -
Autophagy
The Valsa mali effector Vm1G-1794 protects the aggregated MdEF-Tu from autophagic degradation to promote infection in apple. [Abstract]2023 Jun;19(6):1745-1763. PMID: 36449354 -
Autophagy
RNF186 regulates EFNB1 (ephrin B1)-EPHB2-induced autophagy in the colonic epithelial cells for the maintenance of intestinal homeostasis. [Abstract]2021 Oct;17(10):3030-3047. PMID: 33280498 -
Nat Commun
2026 Jun 15. PMID: 42297841 -
Nat Commun
Epstein-Barr virus infection upregulates extracellular OLFM4 to activate YAP signaling during gastric cancer progression. [Abstract]2024 Dec 4;15(1):10543. PMID: 39627192 -
J Adv Res
MyD88 inhibitory Peptide-2 (MIP2) improves neurological outcomes and reduces neuroinflammation after intracerebral Hemorrhage-mediated secondary brain injury. [Abstract]2026 Mar 23:S2090-1232(26)00271-7. PMID: 41881250 -
J Adv Res
Matrix metalloproteinase-9 inhibition prevents aquaporin-4 depolarization-mediated glymphatic dysfunction in Parkinson's disease. [Abstract]2024 Feb:56:125-136. PMID: 36940850 -
Redox Biol
SERPINE1 drives ferroptosis in acute respiratory distress syndrome by disrupting mitochondrial NAD+ homeostasis and suppressing Sirt3 activity. [Abstract]2026 Jul:94:104146. PMID: 42190562 -
Adv Sci (Weinh)
Genome-Wide CRISPR Screen Identifies a microRNA Orchestrating Pleiotropic Resistance to Targeted Therapy and T Cell Immunity in Melanoma. [Abstract]2026 May 26:e15158. PMID: 42189126 -
Adv Sci (Weinh)
IGFBP5 Restores Endometrial Receptivity and Rescues Implantation Failure in Polycystic Ovary Syndrome. [Abstract]2026 Mar 12:e20455. PMID: 41816941 -
MedComm (2020)
Implant Renal Injury-Responsive Cells to Supplement Erythropoietin and Protect Kidney Injury. [Abstract]2025 Oct 30;6(11):e70438. PMID: 41179707 -
Adv Sci (Weinh)
Mechanical Load-Induced Upregulation of Talin2 through Non-Canonical Deubiquitination of OTUB1 Drives Facet Joint Osteoarthritis Pathogenesis. [Abstract]2025 Apr 25:e2501046. PMID: 40279639 -
Adv Sci (Weinh)
Targeting Antigen-Presenting Cells to Enhance the Tumor-Spleen Immunity Cycle through Liposome-Neoantigen Vaccine. [Abstract]2025 Mar 24:e2500021. PMID: 40125791 -
Sci Adv
Multidimensional mapping of stimulation-responsive regulatory elements and candidate causal variants in T cell activation. [Abstract]2026 Jan 2;12(1):eady2539. PMID: 41477821 -
-
Cell Death Dis
Sensing of endogenous retroviruses-derived RNA by ZBP1 triggers PANoptosis in DNA damage and contributes to toxic side effects of chemotherapy. [Abstract]2024 Oct 27;15(10):779. PMID: 39465258 -
Cell Death Dis
Identification of KW-2449 as a dual inhibitor of ferroptosis and necroptosis reveals that autophagy is a targetable pathway for necroptosis inhibitors to prevent ferroptosis. [Abstract]2024 Oct 21;15(10):764. PMID: 39433736 -
Cell Death Dis
4EBP1 senses extracellular glucose deprivation and initiates cell death signaling in lung cancer. [Abstract]2022 Dec 27;13(12):1075. PMID: 36575176 -
Small
Photo-Enhanced CRISPR/Cas9 System Enables Robust PD-L1 Gene Disruption in Cancer Cells and Cancer Stem-Like Cells for Efficient Cancer Immunotherapy. [Abstract]2020 Dec 2;e2004879. PMID: 33289336 -
Cell Commun Signal
Retinoic acid enhances γδ T cell cytotoxicity in nasopharyngeal carcinoma by reversing immune exhaustion. [Abstract]2025 Mar 29;23(1):156. PMID: 40158172 -
Cell Commun Signal
L-Fucose promotes enteric nervous system regeneration in type 1 diabetic mice by inhibiting SMAD2 signaling pathway in enteric neural precursor cells. [Abstract]2023 Oct 5;21(1):273. PMID: 37798789 -
Phytomedicine
JFNE-A isolated from Jing-Fang n-butanol extract attenuates lipopolysaccharide-induced acute lung injury by inhibiting oxidative stress and the NF-κB signaling pathway via promotion of autophagy. [Abstract]2022 Feb:96:153891. PMID: 35026506 -
Cell Death Discov
MSLN-mediated activation of EGFR-ERK1/2 signaling drives liver metastasis in breast cancer. [Abstract]2026 Jan 9;12(1):11. PMID: 41513618 -
J Transl Med
2025 Nov 7;23(1):1243. PMID: 41204251 -
Dev Cell
Very long-chain fatty acids control peroxisome dynamics via a feedback loop in intestinal stem cells during gut regeneration. [Abstract]2024 Jul 16:S1534-5807(24)00398-8. PMID: 39047737 -
Dev Cell
2024 May 17:S1534-5807(24)00296-X. PMID: 38776924 -
Int J Biol Macromol
Targeting glutaryl-CoA dehydrogenase-driven acetyl coenzyme A acetyltransferase 2 crotonylation dysregulates cholesterol metabolism in pancreatic cancer cells. [Abstract]2026 Jan;335(Pt 2):149182. PMID: 41285334 -
Int J Biol Macromol
DTL promotes pancreatic cancer progression through ubiquitin-mediated degradation of SMAD4. [Abstract]2025 Dec;333(Pt 2):148692. PMID: 41203163 -
Neural Regen Res
2024 Dec 16. PMID: 39688577 -
Int J Mol Med
Inhibition of miRNA‑135a‑5p ameliorates TGF‑β1‑induced human renal fibrosis by targeting SIRT1 in diabetic nephropathy. [Abstract]2020 Sep;46(3):1063-1073. PMID: 32705273 -
Free Radic Biol Med
Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease. [Abstract]2026 Jun 6:254:46-61. PMID: 42252064 -
Free Radic Biol Med
Dual Keap1-Dependent actions of pubescenoside A against ulcerative colitis: Promoting DDX5 ubiquitination degradation and Nrf2 nuclear translocation to suppress inflammation. [Abstract]2025 Aug 21:240:426-438. PMID: 40849001 -
Free Radic Biol Med
Cordycepin promotes autophagic degradation of α-synuclein via CacyBP/SIP activation for ameliorating olfactory dysfunction against Parkinson's disease. [Abstract]2025 Oct:238:522-541. PMID: 40639630 -
Free Radic Biol Med
M2 macrophage-derived exosome facilitates aerobic glycolysis and osteogenic differentiation of hPDLSCs by regulating TRIM26-induced PKM ubiquitination. [Abstract]2025 Sep:237:88-100. PMID: 40449810 -
Free Radic Biol Med
Role of ferroptosis mediated by abnormal membrane structure in DEHP-induced reproductive injury. [Abstract]2025 Apr 28:235:150-161. PMID: 40306442 -
EMBO Mol Med
Spleen-targeted neoantigen DNA vaccine for personalized immunotherapy of hepatocellular carcinoma. [Abstract]2023 Oct 11;15(10):e16836. PMID: 37552209 -
Br J Cancer
Remodelling hypoxic TNBC microenvironment restores antitumor efficacy of Vγ9Vδ2 T cell therapy. [Abstract]2025 May 28. PMID: 40437021 -
Cell Rep
Meteorin-like alleviates hepatic steatosis by regulating hepatic triglyceride secretion and fatty acid oxidation. [Abstract]2025 Feb 5;44(2):115246. PMID: 39918960 -
Aging Cell
Identification of AS1842856 as a novel small-molecule GSK3α/β inhibitor against Tauopathy by accelerating GSK3α/β exocytosis. [Abstract]2024 Sep 17:e14336. PMID: 39287420 -
Cell Prolif
Adiponectin receptor agonist AdipoRon blocks skin inflamm-ageing by regulating mitochondrial dynamics. [Abstract]2021 Dec;54(12):e13155. PMID: 34725875 -
Int J Oncol
2026 May;68(5):58. PMID: 41823537 -
Int J Oncol
Notch3 mediated TGF‑β1 activation enhances epithelial‑mesenchymal transition and cancer stemness in non‑small lung cancer. [Abstract]2025 Oct;67(4):85. PMID: 40849808 -
J Med Chem
Rational Design for Nitroreductase (NTR)-Responsive Proteolysis Targeting Chimeras (PROTACs) Selectively Targeting Tumor Tissues. [Abstract]2022 Mar 24;65(6):5057-5071. PMID: 35175763 -
CNS Neurosci Ther
AS1842856 Reduces β-Amyloid Burden via Inhibiting PLA2G4A-Mediated Lysosomal Dysfunction in APP/PS1 Mice. [Abstract]2026 Apr;32(4):e70910. PMID: 42047940 -
Ecotoxicol Environ Saf
A network toxicology and machine learning approach to investigate the mechanism of kidney injury from melamine and cyanuric acid co-exposure. [Abstract]2025 Mar 15:293:118029. PMID: 40088607 -
Biochem Pharmacol
5-Methoxyseselin inhibits neuronal ferroptosis and β-amyloid production in female APP/PS1 transgenic mice. [Abstract]2026 Jun 25;251(Pt 2):118188. PMID: 42349617 -
Biochem Pharmacol
USP5 stabilizes FoxM1 to enhance trophoblast proliferation, invasion and migration in recurrent spontaneous abortion. [Abstract]2026 Jun:248:117825. PMID: 41713621 -
Cell Mol Life Sci
Vitamin B12 ameliorates gut epithelial injury via modulating the HIF-1 pathway and gut microbiota. [Abstract]2024 Sep 11;81(1):397. PMID: 39261351 -
Biochem Pharmacol
BI-7273, a BRD9 inhibitor, reduces lipid accumulation by downregulating the AKT/mTOR/SREBP1 signaling pathway. [Abstract]2024 Jul 4:116412. PMID: 38971334 -
Food Funct
Oleanolic acid, a small-molecule natural product, inhibits ECM degeneration in osteoarthritis by regulating the Hippo/YAP and Wnt/β-catenin pathways. [Abstract]2023 Nov 13;14(22):9999-10013. PMID: 37856220 -
Drug Des Devel Ther
Paroxetine Attenuates Chondrocyte Pyroptosis and Inhibits Osteoclast Formation by Inhibiting NF-κB Pathway Activation to Delay Osteoarthritis Progression. [Abstract]2023 Aug 16:17:2383-2399. PMID: 37605762 -
J Cell Biol
Accumulated precursors of specific GPI-anchored proteins upregulate GPI biosynthesis with ARV1. [Abstract]2023 May 1;222(5):e202208159. PMID: 36828365 -
Neurobiol Dis
Neuronal ACVR1-mediated H3K18 lactylation drives NLRP3 pyroptosis to sustain neuropathic pain via metabolic-epigenetic coupling. [Abstract]2026 Jun 15:224:107397. PMID: 41991084 -
Cells
MK-2206 Alleviates Renal Fibrosis by Suppressing the Akt/mTOR Signaling Pathway In Vivo and In Vitro. [Abstract]2022 Nov 5;11(21):3505. PMID: 36359901 -
ACS Biomater Sci Eng
eIF2α-ATF4 Pathway Activated by a Change in the Calcium Environment Participates in BCP-Mediated Bone Regeneration. [Abstract]2021 Jul 12;7(7):3256-3268. PMID: 34191473 -
EMBO Rep
Plasmodium transcription repressor AP2-O3 regulates sex-specific identity of gene expression in female gametocytes. [Abstract]2021 May 5;22(5):e51660. PMID: 33665945 -
J Inflamm (Lond)
2025 Jan 24;22(1):4. PMID: 39856697 -
Mol Cancer Res
Vasorin exocytosed from glioma cells facilitates angiogenesis via VEGFR2/AKT signaling pathway. [Abstract]2024 Jul 2;22(7):668-681. PMID: 38488456 -
Mol Cancer Res
UBE2C Is Upregulated by Estrogen and Promotes Epithelial-Mesenchymal Transition via p53 in Endometrial Cancer. [Abstract]2020 Feb;18(2):204-215. PMID: 31662448 -
Respir Res
Ubiquitination of angiotensin-converting enzyme 2 contributes to the development of pulmonary arterial hypertension mediated by neural precursor cell-expressed developmentally down-regulated gene 4-Like. [Abstract]2024 Aug 29;25(1):326. PMID: 39210401 -
Pharmaceuticals (Basel)
The Analgesia Effect of Aucubin on CFA-Induced Inflammatory Pain by Inhibiting Glial Cells Activation-Mediated Inflammatory Response via Activating Mitophagy. [Abstract]2023 Nov 1;16(11):1545. PMID: 38004411 -
Eur J Pharmacol
Sampsonione F suppresses adipogenesis via activating p53 pathway during the mitotic clonal expansion progression of adipocyte differentiation. [Abstract]2022 Jun 15;925:175002. PMID: 35526567 -
Int Immunopharmacol
Se-methylselenocysteine alleviates sepsis-associated inflammation by upregulating GPX4 to inhibit macrophage ferroptosis. [Abstract]2026 May 14:182:116851. PMID: 42134291 -
Int Immunopharmacol
Targeting TNF-α: The therapeutic potential of certolizumab pegol in the early period of cerebral ischemia reperfusion injury in mice. [Abstract]2024 Jun 21:137:112498. PMID: 38908079 -
Int J Mol Sci
Quercetin Modulates Ferroptosis via the SIRT1/Nrf-2/HO-1 Pathway and Attenuates Cartilage Destruction in an Osteoarthritis Rat Model. [Abstract]2024 Jul 7;25(13):7461. PMID: 39000568 -
Int Immunopharmacol
2019 Apr:69:143-149. PMID: 30710793 -
Inflammation
Exosomes From Human Umbilical Cord Stem Cells Suppress Macrophage-to-myofibroblast Transition, Alleviating Renal Fibrosis. [Abstract]2024 Dec;47(6):2094-2107. PMID: 38662165 -
Front Pharmacol
Fengreqing Oral Liquid Exerts Anti-Inflammatory Effects by Promoting Apoptosis and Inhibiting PI3K/AKT and NF-κB Signaling Pathways. [Abstract]2022 Mar 14;13:824579. PMID: 35370749 -
Front Pharmacol
Circulating Exosomal miRNAs as Novel Biomarkers Perform Superior Diagnostic Efficiency Compared With Plasma miRNAs for Large-Artery Atherosclerosis Stroke. [Abstract]2021 Nov 26;12:791644. PMID: 34899352 -
Biogerontology
Effect of Hong Huang Tang on memory enhancement and mitigation of microgravity-induced oxidative stress in C. elegans. [Abstract]2025 Dec 16;27(1):24. PMID: 41400846 -
Front Cell Dev Biol
HSP27 Protects Skin From Ultraviolet B -Induced Photodamage by Regulating Autophagy and Reactive Oxygen Species Production. [Abstract]2022 Apr 4;10:852244. PMID: 35445017 -
Front Chem
2021 Dec 3:9:783705. PMID: 34926404 -
Toxicology
The role of β2-AR/PI3K/AKT pathway in the proliferation, migration and invasion of THLE-2 cells induced by nicotine. [Abstract]2024 Aug 13:153924. PMID: 39147091 -
Molecules
Design, Synthesis and Biological Evaluation of Novel and Potent Protein Arginine Methyltransferases 5 Inhibitors for Cancer Therapy. [Abstract]2022 Oct 6;27(19):6637. PMID: 36235174 -
Molecules
Notch3-Mediated mTOR Signaling Pathway Is Involved in High Glucose-Induced Autophagy in Bovine Kidney Epithelial Cells. [Abstract]2022 May 13;27(10):3121. PMID: 35630598 -
Bioorg Chem
Discovery of novel potent covalent inhibitor-based EGFR degrader with excellent in vivo efficacy. [Abstract]2022 Mar:120:105605. PMID: 35081479 -
Molecules
Functional Analysis of the GPI Transamidase Complex by Screening for Amino Acid Mutations in Each Subunit. [Abstract]2021 Sep 8;26(18):5462. PMID: 34576938 -
Biochim Biophys Acta Mol Basis Dis
Hepatic microRNA-320 restrains ferroptosis to mitigate acute-on-chronic alcohol-induced liver injury. [Abstract]2025 Feb 26;1871(4):167748. PMID: 40015214 -
Mol Med Rep
2023 Feb;27(2):45. PMID: 36633134 -
Biochim Biophys Acta Mol Basis Dis
BMAL1 modulates smooth muscle cells phenotypic switch towards fibroblast-like cells and stabilizes atherosclerotic plaques by upregulating YAP1. [Abstract]2022 Sep 1;1868(9):166450. PMID: 35598770 -
Sci Rep
Repulsive guidance molecule A in the resolution of inflammation from ischemic stroke-associated pneumonia in mice. [Abstract]2025 Oct 24;15(1):37197. PMID: 41136476 -
Cancer Sci
2025 Sep 2. PMID: 40897681 -
J Lipid Res
siRNA nanoparticle targeting Usp20 lowers lipid levels and ameliorates metabolic syndrome in mice. [Abstract]2024 Aug 20:100626. PMID: 39173829 -
Sci Rep
GLUT1-mediated glycolysis supports GnRH-induced secretion of luteinizing hormone from female gonadotropes. [Abstract]2020 Aug 3;10(1):13063. PMID: 32747664 -
J Extracell Biol
Enhanced Anti-Tumour Efficacy of iRGD-Modified Cell-Bound Membrane Vesicles (iRGD-CBMVs) as a Novel Drug Carrier. [Abstract]2025 Jun 2;4(6):e70052. PMID: 40458672 -
Cell Signal
Annexin A2 binds the 3'-UTR of H2AX mRNA and regulates histone-H2AX-derived hypoxia-inducible factor 1-alpha activation. [Abstract]2025 Mar 29:111781. PMID: 40164417 -
Cell Signal
KGF impedes TRIM21-enhanced stabilization of keratin 10 mediating differentiation in hypopharyngeal cancer. [Abstract]2025 Jan 21:127:111614. PMID: 39848455 -
-
iScience
Inhibition of soluble epoxide hydrolase ameliorates renal injury in IgA nephropathy by restoring epoxyeicosatrienoic acids. [Abstract]2026 Jun 1;29(6):116200. PMID: 42256278 -
Metabolites
Smilax china L. Extract Alleviates Metabolic-Associated Fatty Liver Disease by Regulating Gut Microbiota and Bile Acid Metabolism. [Abstract]2025 Dec 26;16(1):31. PMID: 41590639 -
ACS Infect Dis
2025 Dec 12;11(12):3420-3434. PMID: 41277028 -
Biologics
LncRNA GAS5 Modulates the Progression of Glioma Through Repressing miR-135b-5p and Upregulating APC. [Abstract]2024 May 23:18:129-142. PMID: 38817552 -
Front Mol Biosci
Novel Insights into the Potential Diagnostic Value of Circulating Exosomal IncRNA-Related Networks in Large Artery Atherosclerotic Stroke. [Abstract]2021 May 21:8:682769. PMID: 34095232 -
J Neurosci
CaMKIIβ-mediated Phosphorylation Enhances Protein Stability of Spastin to Promote Neurite Outgrowth. [Abstract]2025 Aug 6;45(32):e1995242025. PMID: 40645772 -
Wound Repair Regen
Foamy macrophages potentially inhibit tuberculous wound healing by inhibiting the TLRs/NF-κB signalling pathway. [Abstract]2022 May;30(3):376-396. PMID: 35384137 -
J Pharmacol Exp Ther
Inhibitory Effect of Jing-Fang Powder n-Butanol Extract and Its Isolated Fraction D on Lipopolysaccharide-Induced Inflammation in RAW264.7 Cells. [Abstract]2019 Jul;370(1):62-71. PMID: 31068381 -
Fish Shellfish Immunol
Inducible IL-2 production and IL-2+ cell expansion are landmark events for T-cell activation of teleost. [Abstract]2024 May:148:109515. PMID: 38499218 -
Chem Res Toxicol
The Role of HGF/c-Met/PI3K/AKT Pathway Regulated by α7-nAChR/β2-AR in Nicotine-Promoted Malignant Progression of Nonsmall Cell Lung Cancer A549 Cells. [Abstract]2026 Jun 15;39(6):1088-1101. PMID: 42160188 -
J Virol
Nipah virus matrix protein promotes NF-κB activation by targeting multiple signaling modulators. [Abstract]2026 Jun 23;100(6):e0018526. PMID: 42165681 -
Front Biosci (Landmark Ed)
LARP7 Contributes to Glucose-Induced Cardiac Dysfunction, Apoptosis and Fibrosis by Inhibiting the Degradation of STING. [Abstract]2024 Jul 26;29(7):274. PMID: 39082350 -
BMC Cancer
2022 Jul 10;22(1):751. PMID: 35810312 -
J Chromatogr A
2026 Feb 8:1768:466675. PMID: 41512648 -
J Immunol
RNF186/EPHB2 Axis Is Essential in Regulating TNF Signaling for Colorectal Tumorigenesis in Colorectal Epithelial Cells. [Abstract]2022 Nov 1;209(9):1796-1805. PMID: 36130827 -
J Cell Physiol
Neferine suppresses osteoclast differentiation through suppressing NF-κB signal pathway but not MAPKs and promote osteogenesis. [Abstract]2019 Dec;234(12):22960-22971. PMID: 31127627 -
Tumour Virus Res
PRMT5-mediated symmetric dimethylation of SHBs at Arg169 stabilizes SHBs and promotes angiogenesis and tumor growth. [Abstract]2026 Mar 26:21:200340. PMID: 41903748 -
Viruses
Cellular eEF1G Inhibits Porcine Deltacoronavirus Replication by Binding Nsp12 and Disrupting Its Interaction with Viral Genomic RNA. [Abstract]2025 Oct 13;17(10):1369. PMID: 41157639 -
Exp Cell Res
Deficiency in the Rab25 gene leads to a decline in male fertility and testicular injury: Impact on the regulation of germ cell proliferation and apoptosis. [Abstract]2024 Oct 1;442(2):114285. PMID: 39424096 -
Exp Cell Res
2024 Mar 15;436(2):113980. PMID: 38401686 -
Exp Cell Res
NAT10-mediated AXL mRNA N4-acetylcytidine modification promotes pancreatic carcinoma progression. [Abstract]2023 Jul 15;428(2):113620. PMID: 37156457 -
Bioorg Med Chem
Design, synthesis and biological evaluation of the tumor hypoxia-activated PROTACs bearing caged CRBN E3 ligase ligands. [Abstract]2023 Mar 15:82:117237. PMID: 36906965 -
Exp Cell Res
CSMD1 suppresses cancer progression by inhibiting proliferation, epithelial-mesenchymal transition, chemotherapy-resistance and inducing immunosuppression in esophageal squamous cell carcinoma. [Abstract]2022 Aug 15;417(2):113220. PMID: 35623420 -
Front Oncol
Integrated analysis of scRNA-seq and bulk RNA-seq reveals that GPRC5A is an important prognostic gene in pancreatic cancer and is associated with B-cell Infiltration in pancreatic cancer. [Abstract]2024 Apr 3:14:1283164. PMID: 38634049 -
J Leukoc Biol
LncRNA NEAT1_1 suppresses tumor-like biologic behaviors of fibroblast-like synoviocytes by targeting the miR-221-3p/uPAR axis in rheumatoid arthritis. [Abstract]2022 Mar;111(3):641-653. PMID: 34254354 -
Front Oncol
PTIP Inhibits Cell Invasion in Esophageal Squamous Cell Carcinoma via Modulation of EphA2 Expression. [Abstract]2021 Mar 23:11:629916. PMID: 33833989 -
Mol Biol Rep
ILK inhibition reduces osteophyte formation through suppression of osteogenesis in BMSCs via Akt/GSK-3β/β-catenin pathway. [Abstract]2024 Mar 14;51(1):421. PMID: 38483756 -
Tissue Cell
POU6F1 inhibits cervical cancer progression by activating MAOB to block the ERK1/2 pathway. [Abstract]2025 Dec 3:99:103257. PMID: 41371117 -
BMC Vet Res
The combination of high glucose and LPS induces autophagy in bovine kidney epithelial cells via the Notch3/mTOR signaling pathway. [Abstract]2022 Aug 11;18(1):307. PMID: 35953831 -
PeerJ
Exploring the anti-aging effects of fisetin in telomerase-deficient progeria mouse model. [Abstract]2023 Dec 12:11:e16463. PMID: 38107570 -
J Assist Reprod Genet
FXYD1 was identified as a hub gene in recurrent miscarriage and involved in decidualization via regulating Na/K-ATPase activity. [Abstract]2025 Feb;42(2):665-678. PMID: 39730944 -
Cancer Manag Res
Apolipoprotein E (APOE) Promotes Cell Proliferation and Invasion in Glioma via the PI3K/AKT Signaling Pathway. [Abstract]2026 Apr 1:18:594906. PMID: 41947970 -
Biochem Biophys Res Commun
2023 Aug 6:668:8-18. PMID: 37230046 -
Biochem Biophys Res Commun
Anti-inflammatory and analgesic effect of Forsythiaside B on complete Freund's adjuvant-induced inflammatory pain in mice. [Abstract]2023 Feb 19:645:55-60. PMID: 36680937 -
Cell Physiol Biochem
Reduction of NANOG Mediates the Inhibitory Effect of Aspirin on Tumor Growth and Stemness in Colorectal Cancer. [Abstract]2017;44(3):1051-1063. PMID: 29179207 -
Exp Brain Res
USP19 restores mitochondrial function in neurons by deubiquitinating FUS to alleviate trigeminal neuralgia. [Abstract]2025 Dec 16;244(1):13. PMID: 41400721 -
Oncol Lett
CDCA2 acts as an oncogene and induces proliferation of clear cell renal cell carcinoma cells. [Abstract]2020 Mar;19(3):2466-2474. PMID: 32194746 -
J Obstet Gynaecol Res
Estradiol regulates MBOAT1-mediated ferroptosis and participates in the progression of endometriosis. [Abstract]2025 Sep;51(9):e70069. PMID: 40983106 -
Curr Eye Res
2022 Mar;47(3):343-351. PMID: 34766863 -
Cent Eur J Immunol
Inhibition of XPOT promotes breast cancer cell pyroptosis to suppress cancer progression. [Abstract]2025;50(3):248-259. PMID: 41438360 -
J Spinal Cord Med
Magnolol alleviates oxidative stress injury by modulating microglia activation and polarization in an experimental rat model of spinal cord injury. [Abstract]2026 Jun 16:1-12. PMID: 42301700 -
-
Comput Biol Med
Identification of inflammation-related biomarkers and therapeutic targets for neurogenic bladder fibrosis via multi-omics analysis. [Abstract]2025 Jul 5;196(Pt A):110721. PMID: 40618693 -
-
Heliyon
Resistin alleviates lipopolysaccharide-induced inflammation in bovine alveolar macrophages by activating the AMPK/mTOR signaling pathway and autophagy. [Abstract]2024 Sep 18;10(19):e38026. PMID: 39386884 -
Pediatr Discov
SOX9 regulates epithelial-mesenchymal transformation by mediating the Wnt/ β-catenin signaling pathway in hypospadias. [Abstract]2024 Jul 9;2(4):e94. PMID: 40626133 -
Heliyon
Comprehensive landscape of gastric cancer-targeted therapy and identification of CSNK2A1 as a potential target. [Abstract]2024 Aug 18;10(16):e36205. PMID: 39253198 -
Heliyon
Solanesol alleviates CFA-induced chronic inflammatory pain via inhibition of proinflammatory cytokines in spinal glial cells. [Abstract]Heliyon. 2024 Jul 18;10(15):e34870. PMID: 39157324 -
-
-
Braz J Biol
Neuroprotective potential of lignan-rich fraction of Piper cubeba L. by improving antioxidant capacity in the rat's brain. [Abstract]2023 Jan 6:82:e266573. PMID: 36629543 -
-
-
-
-
-
Heliyon
2020 Aug;6(8):e04793. PMID: 32869005
용액&용해도
DMSO : 250 mg/mL (1435.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Isopropanol : 60 mg/mL (344.45 mM; Need ultrasonic)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2 mg/mL (11.48 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2 mg/mL (11.48 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: Corn Oil
Solubility: 20 mg/mL (114.82 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
Male ICR mice weighing 18 to 25 g are used in the assay. PMSF is dissolved in sesame oil and administered i.p. at a volume of 0.1 mL/10 g b.wt. PMSF is always administered 10 min before i.v. anandamide or vehicle injections. Mice are acclimated to the evaluation room overnight without interruption of food or water. After i.v. anandamide or vehicle administration each animal is evaluated as follows: tail-flick latency (antinociception) response at 5 min and spontaneous (locomotor) activity at 5 to 15 min; or core (rectal) temperature at 5 min and ring-immobility (catalepsy) at 5 to 10 min.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
순도&문서
-
Data Sheet (280 KB)
-
SDS (641 KB)
- English - EN (641 KB)
- Français - FR (641 KB)
- Deutsch - DE (641 KB)
- Norwegian - NO (641 KB)
- Español - ES (641 KB)
- Swedish - SV (641 KB)
- Italian - IT (641 KB)
- Korean - KR (641 KB)
- Portuguese - PT (641 KB)
-
Handling Instructions (2659 KB)
References
[1]. Sekar, M.C. and B.D. Roufogalis, Differential effects of phenylmethanesulfonyl fluoride (PMSF) on carbachol and potassium stimulated phosphoinositide turnover and contraction in longitudinal smooth muscle of guinea pig ileum. Cell Calcium, 1984. 5(3): p. [Content Brief]
[2]. Guther, M.L., W.J. Masterson, and M.A. Ferguson, The effects of phenylmethylsulfonyl fluoride on inositol-acylation and fatty acid remodeling in African trypanosomes. J Biol Chem, 1994. 269(28): p. 18694-701. [Content Brief]
[3]. Compton, D.R. and B.R. Martin, The effect of the enzyme inhibitor phenylmethylsulfonyl fluoride on the pharmacological effect of anandamide in the mouse model of cannabimimetic activity. J Pharmacol Exp Ther, 1997. 283(3): p. 1138-43. [Content Brief]
[4]. Asadi Akbarabadi E, et a. PMSF Attenuates Morphine Antinociceptive Tolerance and Dependence in Mice: Its Association with the Oxidative Stress Suppression. Iran J Pharm Res. 2021 Summer;20(3):300-309. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Isopropanol / DMSO | 1 mM | 5.7409 mL | 28.7043 mL | 57.4086 mL | 143.5214 mL |
| 5 mM | 1.1482 mL | 5.7409 mL | 11.4817 mL | 28.7043 mL | |
| 10 mM | 0.5741 mL | 2.8704 mL | 5.7409 mL | 14.3521 mL | |
| 15 mM | 0.3827 mL | 1.9136 mL | 3.8272 mL | 9.5681 mL | |
| 20 mM | 0.2870 mL | 1.4352 mL | 2.8704 mL | 7.1761 mL | |
| 25 mM | 0.2296 mL | 1.1482 mL | 2.2963 mL | 5.7409 mL | |
| 30 mM | 0.1914 mL | 0.9568 mL | 1.9136 mL | 4.7840 mL | |
| 40 mM | 0.1435 mL | 0.7176 mL | 1.4352 mL | 3.5880 mL | |
| 50 mM | 0.1148 mL | 0.5741 mL | 1.1482 mL | 2.8704 mL | |
| 60 mM | 0.0957 mL | 0.4784 mL | 0.9568 mL | 2.3920 mL | |
| 80 mM | 0.0718 mL | 0.3588 mL | 0.7176 mL | 1.7940 mL | |
| 100 mM | 0.0574 mL | 0.2870 mL | 0.5741 mL | 1.4352 mL |
PMSF Related Classifications
HY-B0496 Related Classifications
Powered by Bioz